Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa
ABSTRACT: Background: : Diarrhoeagenic Escherichia coli pose a significant risk to human health. As such, determining the source(s) of these bacteria when isolated from patients with diarrhoea is an important step in disease prevention. Objectives: : To identify the presence of genes coding for vir...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221008626 |
| _version_ | 1818753088202211328 |
|---|---|
| author | Ntando W. Alfinete John Y. Bolukaoto Lee Heine Natasha Potgieter Tobias G. Barnard |
| author_facet | Ntando W. Alfinete John Y. Bolukaoto Lee Heine Natasha Potgieter Tobias G. Barnard |
| author_sort | Ntando W. Alfinete |
| collection | DOAJ |
| description | ABSTRACT: Background: : Diarrhoeagenic Escherichia coli pose a significant risk to human health. As such, determining the source(s) of these bacteria when isolated from patients with diarrhoea is an important step in disease prevention. Objectives: : To identify the presence of genes coding for virulence and phylogroups among E. coli isolated from children hospitalized due to diarrhoea in Limpopo Province, South Africa. Methods: : E. coli isolates were identified using the VITEK-2 automated system. An 11-gene multiplex polymerase chain reaction (PCR) was used to differentiate five pathogenic types of E. coli: enteroaggregative (EAEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC), enteropathogenic (EPEC) and enterotoxigenic (ETEC). The Clermont quadruplex PCR method was used to identify phylogroups of isolates. Results: : From the 133 isolates tested, 79 were confirmed as E. coli. Of these, 19.0% (15/79) were commensals and 81.0% (64/79) were positive for at least one pathotype, of which ETEC was predominant (16.5%, 13/79), followed by EAEC (10.1%, 8/79), EPEC (7.6%, 6/79) and EHEC (2.5%, 2/79). Hybrid pathotypes were also detected and EAEC/ETEC was predominant (25.3%, 20/79). Phylogroup B2 was predominant (30.4%, 24/79), followed by phylogroup B1 (22.8%, 18/79), and phylogroups C and E (both 12.7%, 10/79). Just over 6% (5/79) of isolates were non-typable. Conclusion: : There was a high distribution of diarrhoeagenic E. coli associated with different phylogroups among children living in Limpopo Province, South Africa. This emphasizes the importance of future monitoring of virulence and phylogroup distribution of E. coli isolates in this province in particular and in South Africa as a whole. |
| first_indexed | 2024-12-18T05:01:48Z |
| format | Article |
| id | doaj.art-1df1dc67cde54e8985992dcd27d44813 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-18T05:01:48Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-1df1dc67cde54e8985992dcd27d448132022-12-21T21:20:07ZengElsevierInternational Journal of Infectious Diseases1201-97122022-01-01114226232Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South AfricaNtando W. Alfinete0John Y. Bolukaoto1Lee Heine2Natasha Potgieter3Tobias G. Barnard4Water and Health Research Centre, University of Johannesburg, Doornfontein, South AfricaWater and Health Research Centre, University of Johannesburg, Doornfontein, South AfricaWater and Health Research Centre, University of Johannesburg, Doornfontein, South AfricaSchool of Mathematical and Natural Sciences, University of Venda, Limpopo Province, South AfricaWater and Health Research Centre, University of Johannesburg, Doornfontein, South Africa; Corresponding author. Address: Water and Health Research Centre, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa. Tel.: +27 115596342; fax: +27 115596329ABSTRACT: Background: : Diarrhoeagenic Escherichia coli pose a significant risk to human health. As such, determining the source(s) of these bacteria when isolated from patients with diarrhoea is an important step in disease prevention. Objectives: : To identify the presence of genes coding for virulence and phylogroups among E. coli isolated from children hospitalized due to diarrhoea in Limpopo Province, South Africa. Methods: : E. coli isolates were identified using the VITEK-2 automated system. An 11-gene multiplex polymerase chain reaction (PCR) was used to differentiate five pathogenic types of E. coli: enteroaggregative (EAEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC), enteropathogenic (EPEC) and enterotoxigenic (ETEC). The Clermont quadruplex PCR method was used to identify phylogroups of isolates. Results: : From the 133 isolates tested, 79 were confirmed as E. coli. Of these, 19.0% (15/79) were commensals and 81.0% (64/79) were positive for at least one pathotype, of which ETEC was predominant (16.5%, 13/79), followed by EAEC (10.1%, 8/79), EPEC (7.6%, 6/79) and EHEC (2.5%, 2/79). Hybrid pathotypes were also detected and EAEC/ETEC was predominant (25.3%, 20/79). Phylogroup B2 was predominant (30.4%, 24/79), followed by phylogroup B1 (22.8%, 18/79), and phylogroups C and E (both 12.7%, 10/79). Just over 6% (5/79) of isolates were non-typable. Conclusion: : There was a high distribution of diarrhoeagenic E. coli associated with different phylogroups among children living in Limpopo Province, South Africa. This emphasizes the importance of future monitoring of virulence and phylogroup distribution of E. coli isolates in this province in particular and in South Africa as a whole.http://www.sciencedirect.com/science/article/pii/S1201971221008626Escherichia coli11-plex PCRPhylogroupsQuadruplex PCRVirulence genes |
| spellingShingle | Ntando W. Alfinete John Y. Bolukaoto Lee Heine Natasha Potgieter Tobias G. Barnard Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa International Journal of Infectious Diseases Escherichia coli 11-plex PCR Phylogroups Quadruplex PCR Virulence genes |
| title | Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa |
| title_full | Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa |
| title_fullStr | Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa |
| title_full_unstemmed | Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa |
| title_short | Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa |
| title_sort | virulence and phylogenetic analysis of enteric pathogenic escherichia coli isolated from children with diarrhoea in south africa |
| topic | Escherichia coli 11-plex PCR Phylogroups Quadruplex PCR Virulence genes |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221008626 |
| work_keys_str_mv | AT ntandowalfinete virulenceandphylogeneticanalysisofentericpathogenicescherichiacoliisolatedfromchildrenwithdiarrhoeainsouthafrica AT johnybolukaoto virulenceandphylogeneticanalysisofentericpathogenicescherichiacoliisolatedfromchildrenwithdiarrhoeainsouthafrica AT leeheine virulenceandphylogeneticanalysisofentericpathogenicescherichiacoliisolatedfromchildrenwithdiarrhoeainsouthafrica AT natashapotgieter virulenceandphylogeneticanalysisofentericpathogenicescherichiacoliisolatedfromchildrenwithdiarrhoeainsouthafrica AT tobiasgbarnard virulenceandphylogeneticanalysisofentericpathogenicescherichiacoliisolatedfromchildrenwithdiarrhoeainsouthafrica |