Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

In the present study, a protocol for Agrobacterium tumefaciens-mediated transformation has been optimized for Woodfordia fruticosa (L.) Kurz. Precultured axenic leaf segments were co-cultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with β-glucuronidase (uidA) co...

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Bibliographic Details
Main Authors:	Mallesham Bulle, Deepa Rathakatla, Raghuvardhan Lakkam, Venugopal Rao Kokkirala, Mahender Aileni, Zhang Peng, Sadanandam Abbagani
Format:	Article
Language:	English
Published:	Elsevier 2015-12-01
Series:	Journal of Genetic Engineering and Biotechnology
Subjects:	A. tumefaciens Woodfordia fruticosa Leaf segments β-Glucuronidase hpt Southern blotting
Online Access:	http://www.sciencedirect.com/science/article/pii/S1687157X15000347

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