Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications
Aptamers are short oligonucleotides designed to possess high binding affinity towards specific target compounds (ions, molecules, or cells). Due to their function and unique advantages, aptamers are considered viable alternatives to antibodies as biorecognition elements in bioassays and biosensors....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/18/7786 |
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| author | Anastasios Economou Christos Kokkinos Leda Bousiakou Tibor Hianik |
| author_facet | Anastasios Economou Christos Kokkinos Leda Bousiakou Tibor Hianik |
| author_sort | Anastasios Economou |
| collection | DOAJ |
| description | Aptamers are short oligonucleotides designed to possess high binding affinity towards specific target compounds (ions, molecules, or cells). Due to their function and unique advantages, aptamers are considered viable alternatives to antibodies as biorecognition elements in bioassays and biosensors. On the other hand, paper-based devices (PADs) have emerged as a promising and powerful technology for the fabrication of low-cost analytical tools, mainly intended for on-site and point-of-care applications. The present work aims to provide a comprehensive overview of paper-based aptasensors. The review describes the fabrication methods and working principles of paper-based devices, the properties of aptamers as bioreceptors, the different modes of detection used in conjunction with aptasensing PADs, and representative applications for the detection of ions, small molecules, proteins, and cells. The future challenges and prospects of these devices are also discussed. |
| first_indexed | 2024-03-10T22:02:02Z |
| format | Article |
| id | doaj.art-1df64ecf73344de0a83aff741ab12f4e |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T22:02:02Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1df64ecf73344de0a83aff741ab12f4e2023-11-19T12:54:12ZengMDPI AGSensors1424-82202023-09-012318778610.3390/s23187786Paper-Based Aptasensors: Working Principles, Detection Modes, and ApplicationsAnastasios Economou0Christos Kokkinos1Leda Bousiakou2Tibor Hianik3Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, GreeceIMD Laboratories Co., R&D Section, Lefkippos Technology Park, National Centre for Scientific Research (NCSR) Demokritos, Agia Paraskevi, P.O. Box 60037, 15130 Athens, GreeceDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 84248 Bratislava, SlovakiaAptamers are short oligonucleotides designed to possess high binding affinity towards specific target compounds (ions, molecules, or cells). Due to their function and unique advantages, aptamers are considered viable alternatives to antibodies as biorecognition elements in bioassays and biosensors. On the other hand, paper-based devices (PADs) have emerged as a promising and powerful technology for the fabrication of low-cost analytical tools, mainly intended for on-site and point-of-care applications. The present work aims to provide a comprehensive overview of paper-based aptasensors. The review describes the fabrication methods and working principles of paper-based devices, the properties of aptamers as bioreceptors, the different modes of detection used in conjunction with aptasensing PADs, and representative applications for the detection of ions, small molecules, proteins, and cells. The future challenges and prospects of these devices are also discussed.https://www.mdpi.com/1424-8220/23/18/7786aptasensorsaptamerspaper-based devicesbiosensors |
| spellingShingle | Anastasios Economou Christos Kokkinos Leda Bousiakou Tibor Hianik Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications Sensors aptasensors aptamers paper-based devices biosensors |
| title | Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications |
| title_full | Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications |
| title_fullStr | Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications |
| title_full_unstemmed | Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications |
| title_short | Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications |
| title_sort | paper based aptasensors working principles detection modes and applications |
| topic | aptasensors aptamers paper-based devices biosensors |
| url | https://www.mdpi.com/1424-8220/23/18/7786 |
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