Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer
Peanut sprout extracts reportedly exhibit numerous beneficial effects; however, there are few investigations on the biological effects of peanut sprout extracts cultivated with fermented sawdust medium (PSEFS). Here, we examined whether PSEFS demonstrates antitumor activity against bladder cancer, i...
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MDPI AG
2020-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/23/8758 |
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| author | Hongbeom Park Jun-Hui Song Byungdoo Hwang BoKyung Moon Seok-Joong Yun Wun-Jae Kim Sung-Kwon Moon |
| author_facet | Hongbeom Park Jun-Hui Song Byungdoo Hwang BoKyung Moon Seok-Joong Yun Wun-Jae Kim Sung-Kwon Moon |
| author_sort | Hongbeom Park |
| collection | DOAJ |
| description | Peanut sprout extracts reportedly exhibit numerous beneficial effects; however, there are few investigations on the biological effects of peanut sprout extracts cultivated with fermented sawdust medium (PSEFS). Here, we examined whether PSEFS demonstrates antitumor activity against bladder cancer, in vitro and in vivo. The results showed that PSEFS prohibited the proliferation of bladder cancer T24 cells, with this effect attributed to induction of cell cycle arrest at the G1 phase through reduced expression of cyclins and cyclin-dependent kinases caused by a promotion of p21<sup>WAF1</sup> expression. Additionally, PSEFS induced phosphorylation of p38 mitogen-activated protein kinase. Moreover, PSEFS treatment attenuated the invasive and migratory potential of T24 cells due to decreased matrix metalloproteinase-9 activity combined with downregulation of the transcriptional binding activity of SP1, activator protein -1, and nuclear factor-kappaB. Furthermore, PSEFS (20 mg/kg) attenuated the tumor-growth rate in xenograft mice bearing T24 cells, with an effect equivalent to that of cisplatin and in the absence of toxicity following weight-loss evaluation and hematobiochemical testing of PSEFS-treated mice. These results demonstrated the antitumor efficacy of PSEFS both in vitro and in vivo, thereby reporting it as a potential candidate for development of novel agents against bladder cancer. |
| first_indexed | 2024-03-10T14:15:18Z |
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| id | doaj.art-1df813ca060d434581de5127b166bdea |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T14:15:18Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-1df813ca060d434581de5127b166bdea2023-11-20T23:47:22ZengMDPI AGApplied Sciences2076-34172020-12-011023875810.3390/app10238758Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder CancerHongbeom Park0Jun-Hui Song1Byungdoo Hwang2BoKyung Moon3Seok-Joong Yun4Wun-Jae Kim5Sung-Kwon Moon6Department of Food and Nutrition, Chung-Ang University, Anseong 17546, KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, KoreaDepartment of Urology, Chungbuk National University, Cheongju 361-763, Chungbuk, KoreaDepartment of Urology, Chungbuk National University, Cheongju 361-763, Chungbuk, KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, KoreaPeanut sprout extracts reportedly exhibit numerous beneficial effects; however, there are few investigations on the biological effects of peanut sprout extracts cultivated with fermented sawdust medium (PSEFS). Here, we examined whether PSEFS demonstrates antitumor activity against bladder cancer, in vitro and in vivo. The results showed that PSEFS prohibited the proliferation of bladder cancer T24 cells, with this effect attributed to induction of cell cycle arrest at the G1 phase through reduced expression of cyclins and cyclin-dependent kinases caused by a promotion of p21<sup>WAF1</sup> expression. Additionally, PSEFS induced phosphorylation of p38 mitogen-activated protein kinase. Moreover, PSEFS treatment attenuated the invasive and migratory potential of T24 cells due to decreased matrix metalloproteinase-9 activity combined with downregulation of the transcriptional binding activity of SP1, activator protein -1, and nuclear factor-kappaB. Furthermore, PSEFS (20 mg/kg) attenuated the tumor-growth rate in xenograft mice bearing T24 cells, with an effect equivalent to that of cisplatin and in the absence of toxicity following weight-loss evaluation and hematobiochemical testing of PSEFS-treated mice. These results demonstrated the antitumor efficacy of PSEFS both in vitro and in vivo, thereby reporting it as a potential candidate for development of novel agents against bladder cancer.https://www.mdpi.com/2076-3417/10/23/8758peanut sprout extractsfermented sawdust mediumbladder cancercell cyclep38 MAPKmigration |
| spellingShingle | Hongbeom Park Jun-Hui Song Byungdoo Hwang BoKyung Moon Seok-Joong Yun Wun-Jae Kim Sung-Kwon Moon Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer Applied Sciences peanut sprout extracts fermented sawdust medium bladder cancer cell cycle p38 MAPK migration |
| title | Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer |
| title_full | Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer |
| title_fullStr | Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer |
| title_full_unstemmed | Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer |
| title_short | Evaluation of the In Vitro and In Vivo Antitumor Efficacy of Peanut Sprout Extracts Cultivated with Fermented Sawdust Medium Against Bladder Cancer |
| title_sort | evaluation of the in vitro and in vivo antitumor efficacy of peanut sprout extracts cultivated with fermented sawdust medium against bladder cancer |
| topic | peanut sprout extracts fermented sawdust medium bladder cancer cell cycle p38 MAPK migration |
| url | https://www.mdpi.com/2076-3417/10/23/8758 |
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