Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals
Background: Parkinson’s disease (PD) is associated with progressive neuronal damage and dysfunction. Oxidative stress helps to regulate neurodegenerative and neuronal dysfunction. Natural compounds could attenuate oxidative stress in a variety of neurological disorders. B. juncea is a rich source of...
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| Format: | Article |
| Language: | English |
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IMR Press
2021-11-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/26/11/10.52586/5007 |
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| author | Uzma Saleem Shabana Bibi Muhammad Ajmal Shah Bashir Ahmad Ammara Saleem Zunera Chauhdary Fareeha Anwar Nimra Javaid Sundas Hira Muhammad Furqan Akhtar Ghulam Mujtaba Shah Muhammad Saad Khan Haji Muhammad Muhammad Qasim Mohammad Alqarni Majed A. Algarni Renald Blundell Celia Vargas-De-La-Cruz Oscar Herrera-Calderon Reem Hasaballah Alhasani |
| author_facet | Uzma Saleem Shabana Bibi Muhammad Ajmal Shah Bashir Ahmad Ammara Saleem Zunera Chauhdary Fareeha Anwar Nimra Javaid Sundas Hira Muhammad Furqan Akhtar Ghulam Mujtaba Shah Muhammad Saad Khan Haji Muhammad Muhammad Qasim Mohammad Alqarni Majed A. Algarni Renald Blundell Celia Vargas-De-La-Cruz Oscar Herrera-Calderon Reem Hasaballah Alhasani |
| author_sort | Uzma Saleem |
| collection | DOAJ |
| description | Background: Parkinson’s disease (PD) is associated with progressive neuronal damage and dysfunction. Oxidative stress helps to regulate neurodegenerative and neuronal dysfunction. Natural compounds could attenuate oxidative stress in a variety of neurological disorders. B. juncea is a rich source of antioxidants. The present study aimed to evaluate the therapeutic potential of B. juncea leaves for the treatment of PD by applying behavioral, in vivo and in silico studies. For in vivo studies rats were divided into six groups (n = 6). Group-I served as normal control (vehicle control). Group-II was disease control (haloperidol 1 mg/kg). Group-III was kept as a standard group (L-Dopa 100 mg/kg + carbidopa 25 mg/kg). Groups (IV–VI) were the treatment groups, receiving extract at 200-, 400- and 600 mg/kg doses respectively, for 21 days orally. Results: In vivo study results showed that the extract was found to improve muscles strength, motor coordination, and balance in PD. These behavioral outcomes were consistent with the recovery of endogenous antioxidant defence in biochemical analysis which was further corroborated with histopathological ameliorations. Dopamine levels increased and monoamine oxidase B (MAO-B) levels decreased dose-dependently in the brain during the study. Herein, we performed molecular docking analysis of the proposed extracted phytochemicals has explained that four putative phytochemicals (sinapic acid, rutin, ferulic acid, and caffeic acid) have presented very good results in terms of protein-ligand binding interactions as well as absorption, distribution, metabolism, excretion & toxicity (ADMET) profile estimations. Conclusion: The undertaken study concluded the anti-Parkinson activity of B. juncea and further suggests developments on its isolated compounds in PD therapeutics. |
| first_indexed | 2024-12-21T04:33:03Z |
| format | Article |
| id | doaj.art-1dfa81220c694a63885cb27b4ad9478e |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-12-21T04:33:03Z |
| publishDate | 2021-11-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-1dfa81220c694a63885cb27b4ad9478e2022-12-21T19:15:54ZengIMR PressFrontiers in Bioscience-Landmark2768-67012021-11-0126111031105110.52586/5007s2768-6701(21)00089-7Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicalsUzma Saleem0Shabana Bibi1Muhammad Ajmal Shah2Bashir Ahmad3Ammara Saleem4Zunera Chauhdary5Fareeha Anwar6Nimra Javaid7Sundas Hira8Muhammad Furqan Akhtar9Ghulam Mujtaba Shah10Muhammad Saad Khan11Haji Muhammad12Muhammad Qasim13Mohammad Alqarni14Majed A. Algarni15Renald Blundell16Celia Vargas-De-La-Cruz17Oscar Herrera-Calderon18Reem Hasaballah Alhasani19Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, 38000 Faisalabad, PakistanYunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, 650091 Kunming, Yunnan, ChinaDepartment of Pharmacognosy, Faculty of Pharmaceutical Sciences, Government College University, 38000 Faisalabad, PakistanRiphah Institute of Pharmaceutical Sciences, Riphah International University, 54000 Lahore, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, 38000 Faisalabad, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, 38000 Faisalabad, PakistanRiphah Institute of Pharmaceutical Sciences, Riphah International University, 54000 Lahore, PakistanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, 38000 Faisalabad, PakistanRiphah Institute of Pharmaceutical Sciences, Riphah International University, 54000 Lahore, PakistanRiphah Institute of Pharmaceutical Sciences, Riphah International University, 54000 Lahore, PakistanDepartment of Botany, Faculty of Biological and Health Sciences, Hazara University, 21120 Mansehra, PakistanDepartment of Biosciences, Faculty of Sciences, COMSATS University Islamabad, 57000 Sahiwal, PakistanDepartment of Chemistry, Federal Urdu University of Arts, Science & Technology, 75300 Karachi, PakistanDr. Muhammad Ajmal Khan Institute of Sustainable Halophyte Utilization, University of Karachi, 75270 Karachi, PakistanDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif University, 21944 Taif, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, Taif University, 21944 Taif, Saudi ArabiaDepartment of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, MSD2080 Msida, MaltaDepartment of Pharmacology, Bromatology, Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, 15001 Lima, PeruDepartment of Pharmacology, Bromatology, Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, 15001 Lima, PeruDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, 21961 Makkah, Saudi ArabiaBackground: Parkinson’s disease (PD) is associated with progressive neuronal damage and dysfunction. Oxidative stress helps to regulate neurodegenerative and neuronal dysfunction. Natural compounds could attenuate oxidative stress in a variety of neurological disorders. B. juncea is a rich source of antioxidants. The present study aimed to evaluate the therapeutic potential of B. juncea leaves for the treatment of PD by applying behavioral, in vivo and in silico studies. For in vivo studies rats were divided into six groups (n = 6). Group-I served as normal control (vehicle control). Group-II was disease control (haloperidol 1 mg/kg). Group-III was kept as a standard group (L-Dopa 100 mg/kg + carbidopa 25 mg/kg). Groups (IV–VI) were the treatment groups, receiving extract at 200-, 400- and 600 mg/kg doses respectively, for 21 days orally. Results: In vivo study results showed that the extract was found to improve muscles strength, motor coordination, and balance in PD. These behavioral outcomes were consistent with the recovery of endogenous antioxidant defence in biochemical analysis which was further corroborated with histopathological ameliorations. Dopamine levels increased and monoamine oxidase B (MAO-B) levels decreased dose-dependently in the brain during the study. Herein, we performed molecular docking analysis of the proposed extracted phytochemicals has explained that four putative phytochemicals (sinapic acid, rutin, ferulic acid, and caffeic acid) have presented very good results in terms of protein-ligand binding interactions as well as absorption, distribution, metabolism, excretion & toxicity (ADMET) profile estimations. Conclusion: The undertaken study concluded the anti-Parkinson activity of B. juncea and further suggests developments on its isolated compounds in PD therapeutics.https://www.imrpress.com/journal/FBL/26/11/10.52586/5007antioxidantmolecular dockingneuronal dysfunctionoxidative stresshaloperidoldopamine |
| spellingShingle | Uzma Saleem Shabana Bibi Muhammad Ajmal Shah Bashir Ahmad Ammara Saleem Zunera Chauhdary Fareeha Anwar Nimra Javaid Sundas Hira Muhammad Furqan Akhtar Ghulam Mujtaba Shah Muhammad Saad Khan Haji Muhammad Muhammad Qasim Mohammad Alqarni Majed A. Algarni Renald Blundell Celia Vargas-De-La-Cruz Oscar Herrera-Calderon Reem Hasaballah Alhasani Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals Frontiers in Bioscience-Landmark antioxidant molecular docking neuronal dysfunction oxidative stress haloperidol dopamine |
| title | Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| title_full | Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| title_fullStr | Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| title_full_unstemmed | Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| title_short | Anti-Parkinson’s evaluation of Brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| title_sort | anti parkinson s evaluation of brassica juncea leaf extract and underlying mechanism of its phytochemicals |
| topic | antioxidant molecular docking neuronal dysfunction oxidative stress haloperidol dopamine |
| url | https://www.imrpress.com/journal/FBL/26/11/10.52586/5007 |
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