Characterization of human follicular thyroid cancer cell lines in preclinical mouse models
Follicular thyroid cancer (FTC) is the second most common type of thyroid cancers. In order to develop more effective personalized therapies, it is necessary to thoroughly evaluate patient-derived cell lines in in vivo preclinical models before using them to test new, targeted therapies. This study...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2016-04-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | http://www.endocrineconnections.com/content/5/2/47.full |
| _version_ | 1819056892080553984 |
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| author | Ashley N Reeb Andrea Ziegler Reigh-Yi Lin |
| author_facet | Ashley N Reeb Andrea Ziegler Reigh-Yi Lin |
| author_sort | Ashley N Reeb |
| collection | DOAJ |
| description | Follicular thyroid cancer (FTC) is the second most common type of thyroid cancers. In order to develop more effective personalized therapies, it is necessary to thoroughly evaluate patient-derived cell lines in in vivo preclinical models before using them to test new, targeted therapies. This study evaluates the tumorigenic and metastatic potential of a panel of three human FTC cell lines (WRO, FTC-238, and TT1609-CO2) with defined genetic mutations in two in vivo murine models: an orthotopic thyroid cancer model to study tumor progression and a tail vein injection model to study metastasis. All cell lines developed tumors in the orthotopic model, with take rates of 100%. Notably, WRO-derived tumors grew two to four times faster than tumors arising from the FTC-238 and TT2609-CO2 cell lines. These results mirrored those of a tail vein injection model for lung metastasis: one hundred percent of mice injected with WRO cells in the tail vein exhibited aggressive growth of bilateral lung metastases within 35 days. In contrast, tail vein injection of FTC-238 or TT2609-CO2 cells did not result in lung metastasis. Together, our work demonstrates that these human FTC cell lines display highly varied tumorigenic and metastatic potential in vivo with WRO being the most aggressive cell line in both orthotopic and lung metastasis models. This information will be valuable when selecting cell lines for preclinical drug testing. |
| first_indexed | 2024-12-21T13:30:38Z |
| format | Article |
| id | doaj.art-1dfb619bc3c248f990e6b580ff643ef3 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-12-21T13:30:38Z |
| publishDate | 2016-04-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-1dfb619bc3c248f990e6b580ff643ef32022-12-21T19:02:19ZengBioscientificaEndocrine Connections2049-36142049-36142016-04-01521810.1530/EC-15-0114 Characterization of human follicular thyroid cancer cell lines in preclinical mouse modelsAshley N Reeb0Andrea Ziegler1Reigh-Yi Lin2Department of Otolaryngology, Head and Neck Surgery, Saint Louis University School of Medicine, Saint Louis, Missouri, USADepartment of Otolaryngology, Head and Neck Surgery, Saint Louis University School of Medicine, Saint Louis, Missouri, USADepartment of Otolaryngology, Head and Neck Surgery, Saint Louis University School of Medicine, Saint Louis, Missouri, USAFollicular thyroid cancer (FTC) is the second most common type of thyroid cancers. In order to develop more effective personalized therapies, it is necessary to thoroughly evaluate patient-derived cell lines in in vivo preclinical models before using them to test new, targeted therapies. This study evaluates the tumorigenic and metastatic potential of a panel of three human FTC cell lines (WRO, FTC-238, and TT1609-CO2) with defined genetic mutations in two in vivo murine models: an orthotopic thyroid cancer model to study tumor progression and a tail vein injection model to study metastasis. All cell lines developed tumors in the orthotopic model, with take rates of 100%. Notably, WRO-derived tumors grew two to four times faster than tumors arising from the FTC-238 and TT2609-CO2 cell lines. These results mirrored those of a tail vein injection model for lung metastasis: one hundred percent of mice injected with WRO cells in the tail vein exhibited aggressive growth of bilateral lung metastases within 35 days. In contrast, tail vein injection of FTC-238 or TT2609-CO2 cells did not result in lung metastasis. Together, our work demonstrates that these human FTC cell lines display highly varied tumorigenic and metastatic potential in vivo with WRO being the most aggressive cell line in both orthotopic and lung metastasis models. This information will be valuable when selecting cell lines for preclinical drug testing.http://www.endocrineconnections.com/content/5/2/47.fullfollicular thyroid cancerorthotopicthyroidmetastasismouse model |
| spellingShingle | Ashley N Reeb Andrea Ziegler Reigh-Yi Lin Characterization of human follicular thyroid cancer cell lines in preclinical mouse models Endocrine Connections follicular thyroid cancer orthotopic thyroid metastasis mouse model |
| title | Characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| title_full | Characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| title_fullStr | Characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| title_full_unstemmed | Characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| title_short | Characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| title_sort | characterization of human follicular thyroid cancer cell lines in preclinical mouse models |
| topic | follicular thyroid cancer orthotopic thyroid metastasis mouse model |
| url | http://www.endocrineconnections.com/content/5/2/47.full |
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