Skin bacterial community differences among three species of co-occurring Ranid frogs
Skin microbial communities are an essential part of host health and can play a role in mitigating disease. Host and environmental factors can shape and alter these microbial communities and, therefore, we need to understand to what extent these factors influence microbial communities and how this ca...
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| Format: | Article |
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PeerJ Inc.
2023-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/15556.pdf |
| _version_ | 1827607434284236800 |
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| author | Zachary Gajewski Leah R. Johnson Daniel Medina William W. Crainer Christopher M. Nagy Lisa K. Belden |
| author_facet | Zachary Gajewski Leah R. Johnson Daniel Medina William W. Crainer Christopher M. Nagy Lisa K. Belden |
| author_sort | Zachary Gajewski |
| collection | DOAJ |
| description | Skin microbial communities are an essential part of host health and can play a role in mitigating disease. Host and environmental factors can shape and alter these microbial communities and, therefore, we need to understand to what extent these factors influence microbial communities and how this can impact disease dynamics. Microbial communities have been studied in amphibian systems due to skin microbial communities providing some resistance to the amphibian chytrid fungus, Batrachochytrium dendrobatidis. However, we are only starting to understand how host and environmental factors shape these communities for amphibians. In this study, we examined whether amphibian skin bacterial communities differ among host species, host infection status, host developmental stage, and host habitat. We collected skin swabs from tadpoles and adults of three Ranid frog species (Lithobates spp.) at the Mianus River Gorge Preserve in Bedford, New York, USA, and used 16S rRNA gene amplicon sequencing to determine bacterial community composition. Our analysis suggests amphibian skin bacterial communities change across host developmental stages, as has been documented previously. Additionally, we found that skin bacterial communities differed among Ranid species, with skin communities on the host species captured in streams or bogs differing from the communities of the species captured on land. Thus, habitat use of different species may drive differences in host-associated microbial communities for closely-related host species. |
| first_indexed | 2024-03-09T06:53:53Z |
| format | Article |
| id | doaj.art-1dfbaea212e4478c861775cdc695b450 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:53:53Z |
| publishDate | 2023-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-1dfbaea212e4478c861775cdc695b4502023-12-03T10:08:46ZengPeerJ Inc.PeerJ2167-83592023-07-0111e1555610.7717/peerj.15556Skin bacterial community differences among three species of co-occurring Ranid frogsZachary Gajewski0Leah R. Johnson1Daniel Medina2William W. Crainer3Christopher M. Nagy4Lisa K. Belden5Department of Biological Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, Virginia, United StatesDepartment of Biological Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, Virginia, United StatesDepartment of Biological Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, Virginia, United StatesDepartment of Animal Sciences, Cornell University, Ithaca, New York, United StatesMianus River Gorge Preserve, Bedford, New York, United StatesDepartment of Biological Sciences, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, Virginia, United StatesSkin microbial communities are an essential part of host health and can play a role in mitigating disease. Host and environmental factors can shape and alter these microbial communities and, therefore, we need to understand to what extent these factors influence microbial communities and how this can impact disease dynamics. Microbial communities have been studied in amphibian systems due to skin microbial communities providing some resistance to the amphibian chytrid fungus, Batrachochytrium dendrobatidis. However, we are only starting to understand how host and environmental factors shape these communities for amphibians. In this study, we examined whether amphibian skin bacterial communities differ among host species, host infection status, host developmental stage, and host habitat. We collected skin swabs from tadpoles and adults of three Ranid frog species (Lithobates spp.) at the Mianus River Gorge Preserve in Bedford, New York, USA, and used 16S rRNA gene amplicon sequencing to determine bacterial community composition. Our analysis suggests amphibian skin bacterial communities change across host developmental stages, as has been documented previously. Additionally, we found that skin bacterial communities differed among Ranid species, with skin communities on the host species captured in streams or bogs differing from the communities of the species captured on land. Thus, habitat use of different species may drive differences in host-associated microbial communities for closely-related host species.https://peerj.com/articles/15556.pdfAmphibian skin microbiomeLithobates spp.Amphibian chytrid fungusBatrachochytrium dendrobatidisTadpolesRanid frogs |
| spellingShingle | Zachary Gajewski Leah R. Johnson Daniel Medina William W. Crainer Christopher M. Nagy Lisa K. Belden Skin bacterial community differences among three species of co-occurring Ranid frogs PeerJ Amphibian skin microbiome Lithobates spp. Amphibian chytrid fungus Batrachochytrium dendrobatidis Tadpoles Ranid frogs |
| title | Skin bacterial community differences among three species of co-occurring Ranid frogs |
| title_full | Skin bacterial community differences among three species of co-occurring Ranid frogs |
| title_fullStr | Skin bacterial community differences among three species of co-occurring Ranid frogs |
| title_full_unstemmed | Skin bacterial community differences among three species of co-occurring Ranid frogs |
| title_short | Skin bacterial community differences among three species of co-occurring Ranid frogs |
| title_sort | skin bacterial community differences among three species of co occurring ranid frogs |
| topic | Amphibian skin microbiome Lithobates spp. Amphibian chytrid fungus Batrachochytrium dendrobatidis Tadpoles Ranid frogs |
| url | https://peerj.com/articles/15556.pdf |
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