Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.)
The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the character...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17207 |
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| author | Tatjana Popržen Ivan Nikolić Dijana Krstić-Milošević Branka Uzelac Milana Trifunović-Momčilov Marija Marković Olga Radulović |
| author_facet | Tatjana Popržen Ivan Nikolić Dijana Krstić-Milošević Branka Uzelac Milana Trifunović-Momčilov Marija Marković Olga Radulović |
| author_sort | Tatjana Popržen |
| collection | DOAJ |
| description | The rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four <i>Pseudomonas</i> strains isolated from the rhizosphere of the common duckweed (<i>Lemna minor</i>) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. <i>P. putida</i> A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, <i>P. gessardii</i> C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity). |
| first_indexed | 2024-03-08T20:41:32Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:32Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1dfbcc2a1c0e4de28cacb258ea8f50632023-12-22T14:13:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241720710.3390/ijms242417207Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.)Tatjana Popržen0Ivan Nikolić1Dijana Krstić-Milošević2Branka Uzelac3Milana Trifunović-Momčilov4Marija Marković5Olga Radulović6Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaCenter for Biological Control and Plant Growth Promotion, Faculty of Biology, University of Belgrade, 16 Studentski Trg Street, 11000 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana Street, 11060 Belgrade, SerbiaThe rhizosphere represents a center of complex and dynamic interactions between plants and microbes, resulting in various positive effects on plant growth and development. However, less is known about the effects of indole-3-acetic acid (IAA) on aquatic plants. In this study, we report the characterization of four <i>Pseudomonas</i> strains isolated from the rhizosphere of the common duckweed (<i>Lemna minor</i>) with IAA-degradation and -utilization ability. Our results confirm previous reports on the negative effect of IAA on aquatic plants, contrary to the effect on terrestrial plants. <i>P. putida</i> A3-104/5 demonstrated particularly beneficial traits, as it exhibited not only IAA-degrading and -producing activity but also a positive effect on the doubling time of duckweeds in the presence of IAA, positive chemotaxis in the presence of IAA, increased tolerance to oxidative stress in the presence of IAA and increased biofilm formation related to IAA. Similarly, <i>P. gessardii</i> C31-106/3 significantly shortened the doubling time of duckweeds in the presence of IAA, while having a neutral effect in the absence of IAA. These traits are important in the context of plant–bacteria interactions and highlight the role of IAA as a common metabolite in these interactions, especially in aquatic environments where plants are facing unique challenges compared to their terrestrial counterparts. We conclude that IAA-degrading and -producing strains presented in this study might regulate IAA effects on aquatic plants and confer evolutionary benefits under adverse conditions (e.g., under oxidative stress, excess of IAA or nutrient scarcity).https://www.mdpi.com/1422-0067/24/24/17207indole-3-acetic acidduckweedpseudomonas strainsplant–microbe interactions |
| spellingShingle | Tatjana Popržen Ivan Nikolić Dijana Krstić-Milošević Branka Uzelac Milana Trifunović-Momčilov Marija Marković Olga Radulović Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) International Journal of Molecular Sciences indole-3-acetic acid duckweed pseudomonas strains plant–microbe interactions |
| title | Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) |
| title_full | Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) |
| title_fullStr | Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) |
| title_full_unstemmed | Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) |
| title_short | Characterization of the IAA-Producing and -Degrading <i>Pseudomonas</i> Strains Regulating Growth of the Common Duckweed (<i>Lemna minor</i> L.) |
| title_sort | characterization of the iaa producing and degrading i pseudomonas i strains regulating growth of the common duckweed i lemna minor i l |
| topic | indole-3-acetic acid duckweed pseudomonas strains plant–microbe interactions |
| url | https://www.mdpi.com/1422-0067/24/24/17207 |
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