Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002
Two types of cyanobacterial phycobilisomes (PBS) are present: the hemidiscoidal PBS (CpcG-PBS) and the membrane-bound PBS (CpcL-PBS). Both types of PBS have ferredoxin:NADP<sup>+</sup> oxidoreductase (FNR) attached to the termini of their rods through a CpcD domain. To date, the physiolo...
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MDPI AG
2022-06-01
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| author | Xiying Li Chenhui Huang Peijun Wei Kun Zhang Chunxia Dong Qing Lan Zhenggao Zheng Zhengdong Zhang Jindong Zhao |
| author_facet | Xiying Li Chenhui Huang Peijun Wei Kun Zhang Chunxia Dong Qing Lan Zhenggao Zheng Zhengdong Zhang Jindong Zhao |
| author_sort | Xiying Li |
| collection | DOAJ |
| description | Two types of cyanobacterial phycobilisomes (PBS) are present: the hemidiscoidal PBS (CpcG-PBS) and the membrane-bound PBS (CpcL-PBS). Both types of PBS have ferredoxin:NADP<sup>+</sup> oxidoreductase (FNR) attached to the termini of their rods through a CpcD domain. To date, the physiological significance of the attachment remains unknown. We constructed a mutant (dF338) which contains an FNR lacking the N-terminal CpcD domain in <i>Synechococcus</i> sp. PCC 7002. Isolated CpcG-PBS from dF338 did not contain FNR and the cell extracts of the mutant had a 35 kDa protein cross-reacting to anti-FNR antibodies. dF338 grows normally under photoautotrophic conditions, but little growth was observed under photoheterotrophic conditions. A <i>cpcL</i> (<i>cpcG2</i>) mutant grows extremely slowly under photoheterotrophic conditions while a <i>cpcG</i> (<i>cpcG1</i>) mutant, in which PBS rods could not attach to the cores of the CpcG-PBS, can grow photoheterotrophically, strongly suggesting that the attachment of FNR to CpcL-PBS is critical to photoheterotrophic growth. We show that electron transfer to the plastoquinone pool in dF338 and the <i>cpcL</i> mutant was impaired. We also provide evidence that trimeric photosystem I (PSI) and intact CpcL-PBS with a full-length FNR is critical to plastoquinone reduction. The presence of a NADPH-dehydrogenase (NDH)-CpcL-PBS-PSI trimer supercomplex and its roles are discussed. |
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| spelling | doaj.art-1dfc934fe8ef466791beb45ce4b0fd5c2023-11-30T21:30:09ZengMDPI AGMicroorganisms2076-26072022-06-01107131310.3390/microorganisms10071313Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002Xiying Li0Chenhui Huang1Peijun Wei2Kun Zhang3Chunxia Dong4Qing Lan5Zhenggao Zheng6Zhengdong Zhang7Jindong Zhao8State Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaState Key Laboratory of Protein and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, ChinaTwo types of cyanobacterial phycobilisomes (PBS) are present: the hemidiscoidal PBS (CpcG-PBS) and the membrane-bound PBS (CpcL-PBS). Both types of PBS have ferredoxin:NADP<sup>+</sup> oxidoreductase (FNR) attached to the termini of their rods through a CpcD domain. To date, the physiological significance of the attachment remains unknown. We constructed a mutant (dF338) which contains an FNR lacking the N-terminal CpcD domain in <i>Synechococcus</i> sp. PCC 7002. Isolated CpcG-PBS from dF338 did not contain FNR and the cell extracts of the mutant had a 35 kDa protein cross-reacting to anti-FNR antibodies. dF338 grows normally under photoautotrophic conditions, but little growth was observed under photoheterotrophic conditions. A <i>cpcL</i> (<i>cpcG2</i>) mutant grows extremely slowly under photoheterotrophic conditions while a <i>cpcG</i> (<i>cpcG1</i>) mutant, in which PBS rods could not attach to the cores of the CpcG-PBS, can grow photoheterotrophically, strongly suggesting that the attachment of FNR to CpcL-PBS is critical to photoheterotrophic growth. We show that electron transfer to the plastoquinone pool in dF338 and the <i>cpcL</i> mutant was impaired. We also provide evidence that trimeric photosystem I (PSI) and intact CpcL-PBS with a full-length FNR is critical to plastoquinone reduction. The presence of a NADPH-dehydrogenase (NDH)-CpcL-PBS-PSI trimer supercomplex and its roles are discussed.https://www.mdpi.com/2076-2607/10/7/1313cyanobacteriaFNRNDHphotoheterotrophic growthphycobilisome |
| spellingShingle | Xiying Li Chenhui Huang Peijun Wei Kun Zhang Chunxia Dong Qing Lan Zhenggao Zheng Zhengdong Zhang Jindong Zhao Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 Microorganisms cyanobacteria FNR NDH photoheterotrophic growth phycobilisome |
| title | Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 |
| title_full | Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 |
| title_fullStr | Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 |
| title_full_unstemmed | Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 |
| title_short | Attachment of Ferredoxin: NADP<sup>+</sup> Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium <i>Synechococcus</i> sp. PCC 7002 |
| title_sort | attachment of ferredoxin nadp sup sup oxidoreductase to phycobilisomes is required for photoheterotrophic growth of the cyanobacterium i synechococcus i sp pcc 7002 |
| topic | cyanobacteria FNR NDH photoheterotrophic growth phycobilisome |
| url | https://www.mdpi.com/2076-2607/10/7/1313 |
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