Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule
Background: TD.HK01® consists of a Japanese traditional medicine: Nattokinase (derived from soybean) and three other natural components, namely dried Hirudo medicinalis, Semen Mucuna interrupta and Huperzia carinata. Objectives: TD.HK01® is potentially aimed to the preventive treatment of cardiovasc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031323000684 |
| _version_ | 1827862922207952896 |
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| author | Van Anh Pham Ha Thai Tran Thanh Phuong Mai Lien Huong Nguyen Van Hong Nguyen Thang Huu Nguyen Son Song Bui An Van Vu Ha Thanh Do Quang Vinh Trinh |
| author_facet | Van Anh Pham Ha Thai Tran Thanh Phuong Mai Lien Huong Nguyen Van Hong Nguyen Thang Huu Nguyen Son Song Bui An Van Vu Ha Thanh Do Quang Vinh Trinh |
| author_sort | Van Anh Pham |
| collection | DOAJ |
| description | Background: TD.HK01® consists of a Japanese traditional medicine: Nattokinase (derived from soybean) and three other natural components, namely dried Hirudo medicinalis, Semen Mucuna interrupta and Huperzia carinata. Objectives: TD.HK01® is potentially aimed to the preventive treatment of cardiovascular disease (CVD) in general, and myocardial infarction (MI) in particular. Methods: In this study, a non-invasive method has been used to induce MI in rats, preceded by oral administration of TD.HK01® for 30 consecutive days to determine the potential cardiovascular effect of this pretreatment. Rats were divided into four groups: normal control group, ISO-induced group (isoproterenol (ISO), 150 mg/kg, s.c.) and two groups treated by TD.HK01® in different doses (0.31 and 0.92 g/kg, orally + ISO 150 mg/kg, s.c.). Results: TD.HK01® treated groups showed significant improvement as in abnormal electrocardiography (ECG) alteration ratio, in decreased biochemical specific markers troponin T and in decreased non-specific biochemical markers (LDH and AST) between each of TD.HK01® treated groups and ISO-induced group. The effect on vascular system was also evaluated through serum eNOS quantification and a notable increase in an anti-oxidative enzyme catalase. All three groups, except the normal control one, had pathological lesions in histopathology in different levels - modest injuries in treated groups and more severe in ISO-induced group. Conclusions: It is concluded on the basis of the findings that isoproterenol induced MI in all three groups and TD.HK01® did have its potential protective effect in vivo on cardiovascular system. |
| first_indexed | 2024-03-12T14:01:30Z |
| format | Article |
| id | doaj.art-1dfcc46dc74f43a5a8f0bb0aef671fe4 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-03-12T14:01:30Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-1dfcc46dc74f43a5a8f0bb0aef671fe42023-08-22T04:07:09ZengElsevierPhytomedicine Plus2667-03132023-08-0133100472Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsuleVan Anh Pham0Ha Thai Tran1Thanh Phuong Mai2Lien Huong Nguyen3Van Hong Nguyen4Thang Huu Nguyen5Son Song Bui6An Van Vu7Ha Thanh Do8Quang Vinh Trinh9Hanoi Medical University, 1st Ton That Tung Street, Hanoi 10000, VietnamNational Hospital of Tradional Medicine, Hanoi, VietnamHanoi Medical University, 1st Ton That Tung Street, Hanoi 10000, VietnamSunstar Joint Stock company, Hanoi, VietnamSunstar Joint Stock company, Hanoi, VietnamSunstar Joint Stock company, Hanoi, VietnamSunstar Joint Stock company, Hanoi, VietnamSunstar Joint Stock company, Hanoi, VietnamSunstar Joint Stock company, Hanoi, VietnamHanoi Medical University, 1st Ton That Tung Street, Hanoi 10000, Vietnam; Corresponding author.Background: TD.HK01® consists of a Japanese traditional medicine: Nattokinase (derived from soybean) and three other natural components, namely dried Hirudo medicinalis, Semen Mucuna interrupta and Huperzia carinata. Objectives: TD.HK01® is potentially aimed to the preventive treatment of cardiovascular disease (CVD) in general, and myocardial infarction (MI) in particular. Methods: In this study, a non-invasive method has been used to induce MI in rats, preceded by oral administration of TD.HK01® for 30 consecutive days to determine the potential cardiovascular effect of this pretreatment. Rats were divided into four groups: normal control group, ISO-induced group (isoproterenol (ISO), 150 mg/kg, s.c.) and two groups treated by TD.HK01® in different doses (0.31 and 0.92 g/kg, orally + ISO 150 mg/kg, s.c.). Results: TD.HK01® treated groups showed significant improvement as in abnormal electrocardiography (ECG) alteration ratio, in decreased biochemical specific markers troponin T and in decreased non-specific biochemical markers (LDH and AST) between each of TD.HK01® treated groups and ISO-induced group. The effect on vascular system was also evaluated through serum eNOS quantification and a notable increase in an anti-oxidative enzyme catalase. All three groups, except the normal control one, had pathological lesions in histopathology in different levels - modest injuries in treated groups and more severe in ISO-induced group. Conclusions: It is concluded on the basis of the findings that isoproterenol induced MI in all three groups and TD.HK01® did have its potential protective effect in vivo on cardiovascular system.http://www.sciencedirect.com/science/article/pii/S2667031323000684Isoproterenol-induced myocardial infarctionNattokinaseRat ECGeNOSAnti-oxidant activity |
| spellingShingle | Van Anh Pham Ha Thai Tran Thanh Phuong Mai Lien Huong Nguyen Van Hong Nguyen Thang Huu Nguyen Son Song Bui An Van Vu Ha Thanh Do Quang Vinh Trinh Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule Phytomedicine Plus Isoproterenol-induced myocardial infarction Nattokinase Rat ECG eNOS Anti-oxidant activity |
| title | Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule |
| title_full | Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule |
| title_fullStr | Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule |
| title_full_unstemmed | Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule |
| title_short | Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule |
| title_sort | myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase containing hard capsule |
| topic | Isoproterenol-induced myocardial infarction Nattokinase Rat ECG eNOS Anti-oxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S2667031323000684 |
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