Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow
The aim of this study was the development of a process for filling the pores of a β-tricalcium phosphate ceramic with interconnected porosity with an alginate hydrogel. For filling of the ceramics, solutions of alginate hydrogel precursors with suitable viscosity were chosen as determined by rheomet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/6/4/1085 |
| _version_ | 1811213041262395392 |
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| author | Michael Seidenstuecker Steffen Kissling Juergen Ruehe Norbert P. Suedkamp Hermann O. Mayr Anke Bernstein |
| author_facet | Michael Seidenstuecker Steffen Kissling Juergen Ruehe Norbert P. Suedkamp Hermann O. Mayr Anke Bernstein |
| author_sort | Michael Seidenstuecker |
| collection | DOAJ |
| description | The aim of this study was the development of a process for filling the pores of a β-tricalcium phosphate ceramic with interconnected porosity with an alginate hydrogel. For filling of the ceramics, solutions of alginate hydrogel precursors with suitable viscosity were chosen as determined by rheometry. For loading of the porous ceramics with the gel the samples were placed at the flow chamber and sealed with silicone seals. By using a vacuum induced directional flow, the samples were loaded with alginate solutions. The loading success was controlled by ESEM and fluorescence imaging using a fluorescent dye (FITC) for staining of the gel. After loading of the pores, the alginate is transformed into a hydrogel through crosslinking with CaCl2 solution. The biocompatibility of the obtained composite material was tested with a live dead cell staining by using MG-63 Cells. The loading procedure via vacuum assisted directional flow allowed complete filling of the pores of the ceramics within a few minutes (10 ± 3 min) while loading through simple immersion into the polymer solution or through a conventional vacuum method only gave incomplete filling. |
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| format | Article |
| id | doaj.art-1dfe8c0d80824ab4ba5ee6608add5595 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-12T05:39:33Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-1dfe8c0d80824ab4ba5ee6608add55952022-12-22T03:45:41ZengMDPI AGJournal of Functional Biomaterials2079-49832015-12-01641085109810.3390/jfb6041085jfb6041085Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional FlowMichael Seidenstuecker0Steffen Kissling1Juergen Ruehe2Norbert P. Suedkamp3Hermann O. Mayr4Anke Bernstein5Center for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, GermanyCenter for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, GermanyLaboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering-IMTEK, University of Freiburg, Georges-Koehler-Allee 103, Freiburg D-79110, GermanyCenter for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, GermanyCenter for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, GermanyCenter for Surgery, Department of Orthopedics and Trauma Surgery, Medical Center-University of Freiburg, Hugstetter str. 55, Freiburg D-79106, GermanyThe aim of this study was the development of a process for filling the pores of a β-tricalcium phosphate ceramic with interconnected porosity with an alginate hydrogel. For filling of the ceramics, solutions of alginate hydrogel precursors with suitable viscosity were chosen as determined by rheometry. For loading of the porous ceramics with the gel the samples were placed at the flow chamber and sealed with silicone seals. By using a vacuum induced directional flow, the samples were loaded with alginate solutions. The loading success was controlled by ESEM and fluorescence imaging using a fluorescent dye (FITC) for staining of the gel. After loading of the pores, the alginate is transformed into a hydrogel through crosslinking with CaCl2 solution. The biocompatibility of the obtained composite material was tested with a live dead cell staining by using MG-63 Cells. The loading procedure via vacuum assisted directional flow allowed complete filling of the pores of the ceramics within a few minutes (10 ± 3 min) while loading through simple immersion into the polymer solution or through a conventional vacuum method only gave incomplete filling.http://www.mdpi.com/2079-4983/6/4/1085β-tricalcium phosphateporous ceramicalginatehydrogelflow-chamber |
| spellingShingle | Michael Seidenstuecker Steffen Kissling Juergen Ruehe Norbert P. Suedkamp Hermann O. Mayr Anke Bernstein Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow Journal of Functional Biomaterials β-tricalcium phosphate porous ceramic alginate hydrogel flow-chamber |
| title | Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow |
| title_full | Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow |
| title_fullStr | Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow |
| title_full_unstemmed | Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow |
| title_short | Novel Method for Loading Microporous Ceramics Bone Grafts by Using a Directional Flow |
| title_sort | novel method for loading microporous ceramics bone grafts by using a directional flow |
| topic | β-tricalcium phosphate porous ceramic alginate hydrogel flow-chamber |
| url | http://www.mdpi.com/2079-4983/6/4/1085 |
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