Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus)
Abstract Gene function conservation is crucial in molecular ecology, especially for key traits like growth and maturation in teleost fish. The vgll3 and six6 genes are known to influence age‐at‐maturity in Atlantic salmon, but their impact on other fish species is poorly understood. Here, we investi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.10727 |
| _version_ | 1797448649042558976 |
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| author | Aristotelis Moulistanos Theopisti Nikolaou Smaragda Sismanoglou Konstantinos Gkagkavouzis Nikoleta Karaiskou Efthimia Antonopoulou Alexandros Triantafyllidis Spiros Papakostas |
| author_facet | Aristotelis Moulistanos Theopisti Nikolaou Smaragda Sismanoglou Konstantinos Gkagkavouzis Nikoleta Karaiskou Efthimia Antonopoulou Alexandros Triantafyllidis Spiros Papakostas |
| author_sort | Aristotelis Moulistanos |
| collection | DOAJ |
| description | Abstract Gene function conservation is crucial in molecular ecology, especially for key traits like growth and maturation in teleost fish. The vgll3 and six6 genes are known to influence age‐at‐maturity in Atlantic salmon, but their impact on other fish species is poorly understood. Here, we investigated the association of vgll3 and six6 in the domestication of gilthead seabream and European seabass, both undergoing selective breeding for growth‐related traits in the Mediterranean. We analysed two different sets of samples using two different genotyping approaches. The first dataset comprised farmed and wild populations from Greece, genotyped for SNPs within the two genes (‘gene‐level genotyping’). The second dataset examined 300–600 k SNPs located in the chromosomes of the two genes, derived from a meta‐analysis of a Pool‐Seq experiment involving farmed and wild populations distributed widely across the Mediterranean (‘chromosome‐level genotyping’). The gene‐level analysis revealed a statistically significant allele frequency differences between farmed and wild populations on both genes in each species. This finding was partially supported by the chromosome‐level analysis, identifying highly differentiated regions may be involved in the domestication process at varying distances from the candidate genes. Noteworthy genomic features were found, such as a CpG island in gilthead seabream and novel candidate genes in European seabass, warranting further investigation. These findings support a putative role of vgll3 and six6 in the maturation and growth of gilthead seabream and European seabass, emphasizing the need for further research on their conserved function. |
| first_indexed | 2024-03-09T14:13:26Z |
| format | Article |
| id | doaj.art-1dffe8272eba4562b535495364e4cb30 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-03-09T14:13:26Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-1dffe8272eba4562b535495364e4cb302023-11-29T05:44:08ZengWileyEcology and Evolution2045-77582023-11-011311n/an/a10.1002/ece3.10727Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus)Aristotelis Moulistanos0Theopisti Nikolaou1Smaragda Sismanoglou2Konstantinos Gkagkavouzis3Nikoleta Karaiskou4Efthimia Antonopoulou5Alexandros Triantafyllidis6Spiros Papakostas7Department of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Zoology, School of Biology Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Genetics, Development & Molecular Biology, School of Biology, Faculty of Science Aristotle University of Thessaloniki Thessaloniki GreeceDepartment of Science and Technology International Hellenic University Thessaloniki GreeceAbstract Gene function conservation is crucial in molecular ecology, especially for key traits like growth and maturation in teleost fish. The vgll3 and six6 genes are known to influence age‐at‐maturity in Atlantic salmon, but their impact on other fish species is poorly understood. Here, we investigated the association of vgll3 and six6 in the domestication of gilthead seabream and European seabass, both undergoing selective breeding for growth‐related traits in the Mediterranean. We analysed two different sets of samples using two different genotyping approaches. The first dataset comprised farmed and wild populations from Greece, genotyped for SNPs within the two genes (‘gene‐level genotyping’). The second dataset examined 300–600 k SNPs located in the chromosomes of the two genes, derived from a meta‐analysis of a Pool‐Seq experiment involving farmed and wild populations distributed widely across the Mediterranean (‘chromosome‐level genotyping’). The gene‐level analysis revealed a statistically significant allele frequency differences between farmed and wild populations on both genes in each species. This finding was partially supported by the chromosome‐level analysis, identifying highly differentiated regions may be involved in the domestication process at varying distances from the candidate genes. Noteworthy genomic features were found, such as a CpG island in gilthead seabream and novel candidate genes in European seabass, warranting further investigation. These findings support a putative role of vgll3 and six6 in the maturation and growth of gilthead seabream and European seabass, emphasizing the need for further research on their conserved function.https://doi.org/10.1002/ece3.10727artificial selectioncandidate gene approachfish farminggenome scansmarine teleost |
| spellingShingle | Aristotelis Moulistanos Theopisti Nikolaou Smaragda Sismanoglou Konstantinos Gkagkavouzis Nikoleta Karaiskou Efthimia Antonopoulou Alexandros Triantafyllidis Spiros Papakostas Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) Ecology and Evolution artificial selection candidate gene approach fish farming genome scans marine teleost |
| title | Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) |
| title_full | Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) |
| title_fullStr | Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) |
| title_full_unstemmed | Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) |
| title_short | Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus) |
| title_sort | investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream sparus aurata linnaeus and european seabass dicentrarchus labrax linnaeus |
| topic | artificial selection candidate gene approach fish farming genome scans marine teleost |
| url | https://doi.org/10.1002/ece3.10727 |
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