Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador
<i>Hedyosmum purpurascens</i> is an endemic species found in the Andes of Ecuador and it is characterized by its pleasant smell. In this study, essential oil (EO) from <i>H. purpurascens</i> was obtained by the hydro-distillation method with a Clevenger-type apparatus. The id...
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2366 |
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| author | James Calva Luis Cartuche Leydy Nathaly Castillo Vladimir Morocho |
| author_facet | James Calva Luis Cartuche Leydy Nathaly Castillo Vladimir Morocho |
| author_sort | James Calva |
| collection | DOAJ |
| description | <i>Hedyosmum purpurascens</i> is an endemic species found in the Andes of Ecuador and it is characterized by its pleasant smell. In this study, essential oil (EO) from <i>H. purpurascens</i> was obtained by the hydro-distillation method with a Clevenger-type apparatus. The identification of the chemical composition was carried out by GC–MS and GC–FID in two capillary columns, DB-5ms and HP-INNOWax. A total of 90 compounds were identified, representing more than 98% of the total chemical composition. Germacrene-D, <i>ϒ</i>-terpinene, α-phellandrene, sabinene, <i>O</i>-cymene, 1,8-cineole and <i>α</i>-pinene accounted for more than 59% of the EO composition. The enantioselective analysis of the EO revealed the occurrence of (+)-α-pinene as a pure enantiomer; in addition, four pairs of enantiomers were found (α-phellandrene, o-cymene, limonene and myrcene). The biological activity against microbiological strains and antioxidants and the anticholinesterase properties were also evaluated and the EO showed a moderate anticholinesterase and antioxidant effect, with an IC<sub>50</sub> value of 95.62 ± 1.03 µg/mL and a SC<sub>50</sub> value of 56.38 ± 1.96. A poor antimicrobial effect was observed for all the strains, with MIC values over 1000 µg/mL. Based on our results, the <i>H. purpurasens</i> EO presented remarkable antioxidant and AChE activities. Despite these promising results, further research seems essential to validate the safety of this medicinal species as a function of dose and time. Experimental studies on the mechanisms of action are essential to validate its pharmacological properties. |
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| publishDate | 2023-03-01 |
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| series | Molecules |
| spelling | doaj.art-1e016dfb399340008d396c987bc2b7c82023-11-17T08:15:41ZengMDPI AGMolecules1420-30492023-03-01285236610.3390/molecules28052366Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in EcuadorJames Calva0Luis Cartuche1Leydy Nathaly Castillo2Vladimir Morocho3Departamento de Química, Universidad Técnica Particular de Loja (UTPL), Loja 1101608, EcuadorDepartamento de Química, Universidad Técnica Particular de Loja (UTPL), Loja 1101608, EcuadorCarrera de Ingeniería Química, Universidad Técnica Particular de Loja (UTPL), Loja 1101608, EcuadorDepartamento de Química, Universidad Técnica Particular de Loja (UTPL), Loja 1101608, Ecuador<i>Hedyosmum purpurascens</i> is an endemic species found in the Andes of Ecuador and it is characterized by its pleasant smell. In this study, essential oil (EO) from <i>H. purpurascens</i> was obtained by the hydro-distillation method with a Clevenger-type apparatus. The identification of the chemical composition was carried out by GC–MS and GC–FID in two capillary columns, DB-5ms and HP-INNOWax. A total of 90 compounds were identified, representing more than 98% of the total chemical composition. Germacrene-D, <i>ϒ</i>-terpinene, α-phellandrene, sabinene, <i>O</i>-cymene, 1,8-cineole and <i>α</i>-pinene accounted for more than 59% of the EO composition. The enantioselective analysis of the EO revealed the occurrence of (+)-α-pinene as a pure enantiomer; in addition, four pairs of enantiomers were found (α-phellandrene, o-cymene, limonene and myrcene). The biological activity against microbiological strains and antioxidants and the anticholinesterase properties were also evaluated and the EO showed a moderate anticholinesterase and antioxidant effect, with an IC<sub>50</sub> value of 95.62 ± 1.03 µg/mL and a SC<sub>50</sub> value of 56.38 ± 1.96. A poor antimicrobial effect was observed for all the strains, with MIC values over 1000 µg/mL. Based on our results, the <i>H. purpurasens</i> EO presented remarkable antioxidant and AChE activities. Despite these promising results, further research seems essential to validate the safety of this medicinal species as a function of dose and time. Experimental studies on the mechanisms of action are essential to validate its pharmacological properties.https://www.mdpi.com/1420-3049/28/5/2366<i>Hedyosmum purpurascens</i>germacrene-DantimicrobialCMIanticholinesteraseantioxidant |
| spellingShingle | James Calva Luis Cartuche Leydy Nathaly Castillo Vladimir Morocho Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador Molecules <i>Hedyosmum purpurascens</i> germacrene-D antimicrobial CMI anticholinesterase antioxidant |
| title | Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador |
| title_full | Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador |
| title_fullStr | Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador |
| title_full_unstemmed | Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador |
| title_short | Biological Activities and Chemical Composition of Essential Oil from <i>Hedyosmum purpurascens</i> (Todzia)—An Endemic Plant in Ecuador |
| title_sort | biological activities and chemical composition of essential oil from i hedyosmum purpurascens i todzia an endemic plant in ecuador |
| topic | <i>Hedyosmum purpurascens</i> germacrene-D antimicrobial CMI anticholinesterase antioxidant |
| url | https://www.mdpi.com/1420-3049/28/5/2366 |
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