Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses
The immune response to vaccines is complex and results in various outcomes. BCG vaccination induces innate and specific responses that can lead to protection against tuberculosis, and cross-protection against other infections. NK cells have been associated with BCG-induced protection. Therefore, we...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/8/1297 |
| _version_ | 1827728529760976896 |
|---|---|
| author | Adeliane Castro da Costa Lília Cristina de Souza Barbosa André Kipnis Ana Paula Junqueira-Kipnis |
| author_facet | Adeliane Castro da Costa Lília Cristina de Souza Barbosa André Kipnis Ana Paula Junqueira-Kipnis |
| author_sort | Adeliane Castro da Costa |
| collection | DOAJ |
| description | The immune response to vaccines is complex and results in various outcomes. BCG vaccination induces innate and specific responses that can lead to protection against tuberculosis, and cross-protection against other infections. NK cells have been associated with BCG-induced protection. Therefore, we hypothesize that differences in NK cell status before BCG vaccination may have a role in the ability of BCG to activate the immune response. Participants of a clinical trial were evaluated after BCG vaccination. The participants were assigned to different groups according to variation in IFN-γ expression by NK cells between days 1 and 15 after BCG vaccination. Individuals that presented a higher increase in IFN-γ expression by NK cells presented reduced CD314 expression at day 1, and after vaccination an increase in inflammatory NK cells and CD4 T-cell expression of IL-17. A negative correlation between expression of CD314 at day 1 and that of IFN-γ by NK cells after BCG vaccination was observed. Participants with lower of IFN-γ expression by NK cells after BCG vaccination presented an increase in the cytotoxic NK subpopulation and CD4 T-cell expression of IL-17 and IFN-γ. In conclusion, the expression of CD314 by NK cells before BCG vaccination influences their IFN-γ responses, generation of NK subpopulations, and the specific T immune response at 15 days after vaccination. |
| first_indexed | 2024-03-10T23:31:21Z |
| format | Article |
| id | doaj.art-1e018bdaaf354cabb290d1cb46633cf2 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T23:31:21Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-1e018bdaaf354cabb290d1cb46633cf22023-11-19T03:17:06ZengMDPI AGVaccines2076-393X2023-07-01118129710.3390/vaccines11081297Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune ResponsesAdeliane Castro da Costa0Lília Cristina de Souza Barbosa1André Kipnis2Ana Paula Junqueira-Kipnis3Campus Goiânia, Goiás Estácio de Sá University, Goiânia 74063-010, ZC, BrazilDepartment of Biosciences and Technology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia 74605-050, ZC, BrazilDepartment of Biosciences and Technology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia 74605-050, ZC, BrazilDepartment of Biosciences and Technology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia 74605-050, ZC, BrazilThe immune response to vaccines is complex and results in various outcomes. BCG vaccination induces innate and specific responses that can lead to protection against tuberculosis, and cross-protection against other infections. NK cells have been associated with BCG-induced protection. Therefore, we hypothesize that differences in NK cell status before BCG vaccination may have a role in the ability of BCG to activate the immune response. Participants of a clinical trial were evaluated after BCG vaccination. The participants were assigned to different groups according to variation in IFN-γ expression by NK cells between days 1 and 15 after BCG vaccination. Individuals that presented a higher increase in IFN-γ expression by NK cells presented reduced CD314 expression at day 1, and after vaccination an increase in inflammatory NK cells and CD4 T-cell expression of IL-17. A negative correlation between expression of CD314 at day 1 and that of IFN-γ by NK cells after BCG vaccination was observed. Participants with lower of IFN-γ expression by NK cells after BCG vaccination presented an increase in the cytotoxic NK subpopulation and CD4 T-cell expression of IL-17 and IFN-γ. In conclusion, the expression of CD314 by NK cells before BCG vaccination influences their IFN-γ responses, generation of NK subpopulations, and the specific T immune response at 15 days after vaccination.https://www.mdpi.com/2076-393X/11/8/1297vaccineinnate immunityheterogeneity responsetuberculosisneutrophiladaptive |
| spellingShingle | Adeliane Castro da Costa Lília Cristina de Souza Barbosa André Kipnis Ana Paula Junqueira-Kipnis Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses Vaccines vaccine innate immunity heterogeneity response tuberculosis neutrophil adaptive |
| title | Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses |
| title_full | Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses |
| title_fullStr | Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses |
| title_full_unstemmed | Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses |
| title_short | Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-γ after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses |
| title_sort | decreased expression of cd314 by nk cells correlates with their ability to respond by producing ifn γ after bcg moscow vaccination and is associated with distinct early immune responses |
| topic | vaccine innate immunity heterogeneity response tuberculosis neutrophil adaptive |
| url | https://www.mdpi.com/2076-393X/11/8/1297 |
| work_keys_str_mv | AT adelianecastrodacosta decreasedexpressionofcd314bynkcellscorrelateswiththeirabilitytorespondbyproducingifngafterbcgmoscowvaccinationandisassociatedwithdistinctearlyimmuneresponses AT liliacristinadesouzabarbosa decreasedexpressionofcd314bynkcellscorrelateswiththeirabilitytorespondbyproducingifngafterbcgmoscowvaccinationandisassociatedwithdistinctearlyimmuneresponses AT andrekipnis decreasedexpressionofcd314bynkcellscorrelateswiththeirabilitytorespondbyproducingifngafterbcgmoscowvaccinationandisassociatedwithdistinctearlyimmuneresponses AT anapaulajunqueirakipnis decreasedexpressionofcd314bynkcellscorrelateswiththeirabilitytorespondbyproducingifngafterbcgmoscowvaccinationandisassociatedwithdistinctearlyimmuneresponses |