Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars
Modern transplantology is in need of transplants. To solve this problem, the use of animal organs and tissues for grafting to humans (xenografts) has been proposed. However, the progress in this direction is hampered by the risk of zoogenous infection of recipients. With regard to economic and ethic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2017-02-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/846 |
| _version_ | 1797214128416227328 |
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| author | R. B. Aitnazarov N. S. Yudin R. S. Kiril’chuk N. N. Kochnev S. P. Knyazev M. I. Voevoda |
| author_facet | R. B. Aitnazarov N. S. Yudin R. S. Kiril’chuk N. N. Kochnev S. P. Knyazev M. I. Voevoda |
| author_sort | R. B. Aitnazarov |
| collection | DOAJ |
| description | Modern transplantology is in need of transplants. To solve this problem, the use of animal organs and tissues for grafting to humans (xenografts) has been proposed. However, the progress in this direction is hampered by the risk of zoogenous infection of recipients. With regard to economic and ethical criteria and to the anatomical and physiological similarity to humans, the pig is the best source of xenografts. The pig genome harbors type A porcine endogenous retroviruses (PERV), which can infect human cell lines in vitro. A population of Siberian minipigs was raised at the Institute of Cytology and Genetics just for xenografting. The goal of the present study is to analyze the copy numbers of PERV A in Siberian minipigs, their founder breeds Landrace and Large White, and wild boars. The copy numbers of PERVs have been determined by absolute measurement with SYBR Green dye. End-point dilutions of a sample with a known copy number have been used for reference. The PERV A copy numbers in standard samples of Siberian minipig DNA are 2.4, 3.6, and 4.9 per cell, which is consistent with data obtained by other scientists. Minipigs and wild boars show a significant difference in retrovirus copy numbers. Thus, the Siberian minipig genome has a considerable number of type A PERVs, conceivably pathogenic to humans. It is necessary to select animals with minimum PERV numbers in the genome for xenografting. The method of PERV A quantitation with SYBR Green allows detection of such animals and selection of Siberian minipigs for reduction of this index. |
| first_indexed | 2024-03-07T16:06:51Z |
| format | Article |
| id | doaj.art-1e04dd28e2f344faad9f994c0481f4fc |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:09:15Z |
| publishDate | 2017-02-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-1e04dd28e2f344faad9f994c0481f4fc2024-04-11T15:30:57ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592017-02-0120675676110.18699/VJ16.192538Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boarsR. B. Aitnazarov0N. S. Yudin1R. S. Kiril’chuk2N. N. Kochnev3S. P. Knyazev4M. I. Voevoda5Institute of Cytology and Genetics SB RAS Novosibirsk State UniversityInstitute of Cytology and Genetics SB RAS Novosibirsk State UniversityNovosibirsk State UniversityNovosibirsk State Agrarian UniversityNovosibirsk State Agrarian UniversityInstitute of Cytology and Genetics SB RAS Novosibirsk State University Research Institute of Internal and Preventive Medicine SB RAMSModern transplantology is in need of transplants. To solve this problem, the use of animal organs and tissues for grafting to humans (xenografts) has been proposed. However, the progress in this direction is hampered by the risk of zoogenous infection of recipients. With regard to economic and ethical criteria and to the anatomical and physiological similarity to humans, the pig is the best source of xenografts. The pig genome harbors type A porcine endogenous retroviruses (PERV), which can infect human cell lines in vitro. A population of Siberian minipigs was raised at the Institute of Cytology and Genetics just for xenografting. The goal of the present study is to analyze the copy numbers of PERV A in Siberian minipigs, their founder breeds Landrace and Large White, and wild boars. The copy numbers of PERVs have been determined by absolute measurement with SYBR Green dye. End-point dilutions of a sample with a known copy number have been used for reference. The PERV A copy numbers in standard samples of Siberian minipig DNA are 2.4, 3.6, and 4.9 per cell, which is consistent with data obtained by other scientists. Minipigs and wild boars show a significant difference in retrovirus copy numbers. Thus, the Siberian minipig genome has a considerable number of type A PERVs, conceivably pathogenic to humans. It is necessary to select animals with minimum PERV numbers in the genome for xenografting. The method of PERV A quantitation with SYBR Green allows detection of such animals and selection of Siberian minipigs for reduction of this index.https://vavilov.elpub.ru/jour/article/view/846pigsiberian mini piglarge white breedlandras breedwild boarporcine endogenous retrovirusdnaenva genecopy numberxenotransplantation. |
| spellingShingle | R. B. Aitnazarov N. S. Yudin R. S. Kiril’chuk N. N. Kochnev S. P. Knyazev M. I. Voevoda Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars Вавиловский журнал генетики и селекции pig siberian mini pig large white breed landras breed wild boar porcine endogenous retrovirus dna enva gene copy number xenotransplantation. |
| title | Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars |
| title_full | Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars |
| title_fullStr | Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars |
| title_full_unstemmed | Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars |
| title_short | Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars |
| title_sort | determination of the copy numbers of type a porcine endogenous retroviruses in domestic pigs and wild boars |
| topic | pig siberian mini pig large white breed landras breed wild boar porcine endogenous retrovirus dna enva gene copy number xenotransplantation. |
| url | https://vavilov.elpub.ru/jour/article/view/846 |
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