谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis
旨在为脂肪酸高产菌株的筛选和构建奠定理论基础，研究谷氨酸棒杆菌脂肪酸合成酶A（FasA）对大肠杆菌脂肪酸合成的影响，利用生物信息学软件预测分析了FasA的性质并构建过表达载体pXMJ19-fasA，转化至大肠杆菌进行表达,对表达诱导剂异丙基-β-D-硫代吡喃半乳糖苷（IPTG）浓度进行考察，并测定了异源表达后的脂肪酸组成。结果表明，重组菌株在IPTG浓度为1 mmol/L下诱导20 h，棕榈油酸和油酸产量分别达到1.735、2.325 mg/g，与对照菌株相比分别提高了53.95%和59.68%。研究结果说明FasA对大肠杆菌合成棕榈油酸和油酸有促进作用。 In order to lay a...
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| Format: | Article |
| Language: | English |
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中粮工科（西安）国际工程有限公司
2023-04-01
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| Series: | Zhongguo youzhi |
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| Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230417&year_id=2023&quarter_id=4&falg=1 |
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| author | 金晶晶，孙子羽，满都拉，刘瑾，胡晨玥，陈忠军JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun |
| author_facet | 金晶晶，孙子羽，满都拉，刘瑾，胡晨玥，陈忠军JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun |
| author_sort | 金晶晶，孙子羽，满都拉，刘瑾，胡晨玥，陈忠军JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun |
| collection | DOAJ |
| description | 旨在为脂肪酸高产菌株的筛选和构建奠定理论基础，研究谷氨酸棒杆菌脂肪酸合成酶A（FasA）对大肠杆菌脂肪酸合成的影响，利用生物信息学软件预测分析了FasA的性质并构建过表达载体pXMJ19-fasA，转化至大肠杆菌进行表达,对表达诱导剂异丙基-β-D-硫代吡喃半乳糖苷（IPTG）浓度进行考察，并测定了异源表达后的脂肪酸组成。结果表明，重组菌株在IPTG浓度为1 mmol/L下诱导20 h，棕榈油酸和油酸产量分别达到1.735、2.325 mg/g，与对照菌株相比分别提高了53.95%和59.68%。研究结果说明FasA对大肠杆菌合成棕榈油酸和油酸有促进作用。
In order to lay a theoretical foundation for screening and constructing fatty acid high-yielding strains, the effects of Corynebacterium glutamicum fatty acid synthase A (FasA) on the synthesis of fatty acids in E. coli were studied, the properties of FasA were predicted and analyzed using bioinformatics software, moreover the overexpression vector pXMJ19-fasA was constructed and transformed into E. coli for expression, the concentration of expression inducer isopropyl-β-D-thiogalactoside (IPTG) was investigated and the fatty acid composition after heterologous expression was determined.The results showed that when the recombinant strain was induced under the IPTG concentration of 1 mmol/L for 20 h，the yield of palmitoleic acid and oleic acid reached 1.735，2.325 mg/g ，which increased by 53.95% and 59.68% respectively compared with the control strain. These results indicate that FasA can promote the synthesis of palmitoleic acid and oleic acid by E. coli. |
| first_indexed | 2024-03-13T02:36:00Z |
| format | Article |
| id | doaj.art-1e09496ba5284be79b672fbb47440b59 |
| institution | Directory Open Access Journal |
| issn | 1003-7969 |
| language | English |
| last_indexed | 2024-03-13T02:36:00Z |
| publishDate | 2023-04-01 |
| publisher | 中粮工科（西安）国际工程有限公司 |
| record_format | Article |
| series | Zhongguo youzhi |
| spelling | doaj.art-1e09496ba5284be79b672fbb47440b592023-06-29T01:18:48Zeng中粮工科（西安）国际工程有限公司Zhongguo youzhi1003-79692023-04-0148494104https://doi.org/10.19902/j.cnki.zgyz.1003-7969.220169谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis金晶晶，孙子羽，满都拉，刘瑾，胡晨玥，陈忠军JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun0内蒙古农业大学 食品科学与工程学院，呼和浩特 010018College of Food Science and Engineering,Inner Mongolia Agricultural University，Hohhot 010018，China)旨在为脂肪酸高产菌株的筛选和构建奠定理论基础，研究谷氨酸棒杆菌脂肪酸合成酶A（FasA）对大肠杆菌脂肪酸合成的影响，利用生物信息学软件预测分析了FasA的性质并构建过表达载体pXMJ19-fasA，转化至大肠杆菌进行表达,对表达诱导剂异丙基-β-D-硫代吡喃半乳糖苷（IPTG）浓度进行考察，并测定了异源表达后的脂肪酸组成。结果表明，重组菌株在IPTG浓度为1 mmol/L下诱导20 h，棕榈油酸和油酸产量分别达到1.735、2.325 mg/g，与对照菌株相比分别提高了53.95%和59.68%。研究结果说明FasA对大肠杆菌合成棕榈油酸和油酸有促进作用。 In order to lay a theoretical foundation for screening and constructing fatty acid high-yielding strains, the effects of Corynebacterium glutamicum fatty acid synthase A (FasA) on the synthesis of fatty acids in E. coli were studied, the properties of FasA were predicted and analyzed using bioinformatics software, moreover the overexpression vector pXMJ19-fasA was constructed and transformed into E. coli for expression, the concentration of expression inducer isopropyl-β-D-thiogalactoside (IPTG) was investigated and the fatty acid composition after heterologous expression was determined.The results showed that when the recombinant strain was induced under the IPTG concentration of 1 mmol/L for 20 h，the yield of palmitoleic acid and oleic acid reached 1.735，2.325 mg/g ，which increased by 53.95% and 59.68% respectively compared with the control strain. These results indicate that FasA can promote the synthesis of palmitoleic acid and oleic acid by E. coli.http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230417&year_id=2023&quarter_id=4&falg=1脂肪酸合成酶；谷氨酸棒杆菌;棕榈油酸；油酸；异源表达fatty acid synthase；corynebacterium glutamicum；palmitoleic acid；oleic acid；heterologous expression |
| spellingShingle | 金晶晶，孙子羽，满都拉，刘瑾，胡晨玥，陈忠军JIN Jingjing，SUN Ziyu，MAN Dula，LIU Jin，HU Chenyue，CHEN Zhongjun 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis Zhongguo youzhi 脂肪酸合成酶；谷氨酸棒杆菌;棕榈油酸；油酸；异源表达 fatty acid synthase；corynebacterium glutamicum；palmitoleic acid；oleic acid；heterologous expression |
| title | 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| title_full | 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| title_fullStr | 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| title_full_unstemmed | 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| title_short | 谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达 对脂肪酸合成的影响Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| title_sort | 谷氨酸棒杆菌脂肪酸合成酶fasa基因的异源表达 对脂肪酸合成的影响effect of heterologous expression of fasa gene of corynebacterium glutamicum fatty acid synthase on fatty acid synthesis |
| topic | 脂肪酸合成酶；谷氨酸棒杆菌;棕榈油酸；油酸；异源表达 fatty acid synthase；corynebacterium glutamicum；palmitoleic acid；oleic acid；heterologous expression |
| url | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230417&year_id=2023&quarter_id=4&falg=1 |
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