Heterogeneous Nucleation in Protein Crystallization
Protein crystallization was first discovered in the nineteenth century and has been studied for nearly 200 years. Protein crystallization technology has recently been widely used in many fields, such as drug purification and protein structure analysis. The key to successful crystallization of protei...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/8/1/68 |
| _version_ | 1827751078434701312 |
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| author | Hao Liu Yue Zhao Jing Sun |
| author_facet | Hao Liu Yue Zhao Jing Sun |
| author_sort | Hao Liu |
| collection | DOAJ |
| description | Protein crystallization was first discovered in the nineteenth century and has been studied for nearly 200 years. Protein crystallization technology has recently been widely used in many fields, such as drug purification and protein structure analysis. The key to successful crystallization of proteins is the nucleation in the protein solution, which can be influenced by many factors, such as the precipitating agent, temperature, solution concentration, pH, etc., among which the role of the precipitating agent is extremely important. In this regard, we summarize the nucleation theory of protein crystallization, including classical nucleation theory, two-step nucleation theory, and heterogeneous nucleation theory. We focus on a variety of efficient heterogeneous nucleating agents and crystallization methods as well. The application of protein crystals in crystallography and biopharmaceutical fields is further discussed. Finally, the bottleneck of protein crystallization and the prospect of future technology development are reviewed. |
| first_indexed | 2024-03-11T06:52:15Z |
| format | Article |
| id | doaj.art-1e0b4ea624054c2eb8f8c26f7334f2d8 |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-11T06:52:15Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-1e0b4ea624054c2eb8f8c26f7334f2d82023-11-17T09:49:49ZengMDPI AGBiomimetics2313-76732023-02-01816810.3390/biomimetics8010068Heterogeneous Nucleation in Protein CrystallizationHao Liu0Yue Zhao1Jing Sun2Key Laboratory of Biobased Polymer Materials, College of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaKey Laboratory of Biobased Polymer Materials, College of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, ChinaProtein crystallization was first discovered in the nineteenth century and has been studied for nearly 200 years. Protein crystallization technology has recently been widely used in many fields, such as drug purification and protein structure analysis. The key to successful crystallization of proteins is the nucleation in the protein solution, which can be influenced by many factors, such as the precipitating agent, temperature, solution concentration, pH, etc., among which the role of the precipitating agent is extremely important. In this regard, we summarize the nucleation theory of protein crystallization, including classical nucleation theory, two-step nucleation theory, and heterogeneous nucleation theory. We focus on a variety of efficient heterogeneous nucleating agents and crystallization methods as well. The application of protein crystals in crystallography and biopharmaceutical fields is further discussed. Finally, the bottleneck of protein crystallization and the prospect of future technology development are reviewed.https://www.mdpi.com/2313-7673/8/1/68protein crystallizationcrystallographyheterogeneous nucleationcrystallization strategy |
| spellingShingle | Hao Liu Yue Zhao Jing Sun Heterogeneous Nucleation in Protein Crystallization Biomimetics protein crystallization crystallography heterogeneous nucleation crystallization strategy |
| title | Heterogeneous Nucleation in Protein Crystallization |
| title_full | Heterogeneous Nucleation in Protein Crystallization |
| title_fullStr | Heterogeneous Nucleation in Protein Crystallization |
| title_full_unstemmed | Heterogeneous Nucleation in Protein Crystallization |
| title_short | Heterogeneous Nucleation in Protein Crystallization |
| title_sort | heterogeneous nucleation in protein crystallization |
| topic | protein crystallization crystallography heterogeneous nucleation crystallization strategy |
| url | https://www.mdpi.com/2313-7673/8/1/68 |
| work_keys_str_mv | AT haoliu heterogeneousnucleationinproteincrystallization AT yuezhao heterogeneousnucleationinproteincrystallization AT jingsun heterogeneousnucleationinproteincrystallization |