Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite
The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that re...
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MDPI AG
2018-05-01
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| author | Simona Marc Danijela Kirovski Călin Mircu Ioan Hutu Gabriel Otavă Cristina Paul Oana Maria Boldura Camelia Tulcan |
| author_facet | Simona Marc Danijela Kirovski Călin Mircu Ioan Hutu Gabriel Otavă Cristina Paul Oana Maria Boldura Camelia Tulcan |
| author_sort | Simona Marc |
| collection | DOAJ |
| description | The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of γ-globulins, β-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%. |
| first_indexed | 2024-12-21T20:47:19Z |
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| last_indexed | 2024-12-21T20:47:19Z |
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| series | Molecules |
| spelling | doaj.art-1e0f980f97e548b99d17335b0d3683d52022-12-21T18:50:47ZengMDPI AGMolecules1420-30492018-05-01236127810.3390/molecules23061278molecules23061278Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with ClinoptiloliteSimona Marc0Danijela Kirovski1Călin Mircu2Ioan Hutu3Gabriel Otavă4Cristina Paul5Oana Maria Boldura6Camelia Tulcan7Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaFaculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodenja 18, 11000 Belgrade, SerbiaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaFaculty of Industrial Chemistry and Environmental, University Politehnica Timisoara, Carol Telbisz 6, 300001 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine “King Michael I” of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, RomaniaThe objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of γ-globulins, β-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.http://www.mdpi.com/1420-3049/23/6/1278electrophoresiscalvesclinoptiloliteγ-globulin |
| spellingShingle | Simona Marc Danijela Kirovski Călin Mircu Ioan Hutu Gabriel Otavă Cristina Paul Oana Maria Boldura Camelia Tulcan Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite Molecules electrophoresis calves clinoptilolite γ-globulin |
| title | Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite |
| title_full | Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite |
| title_fullStr | Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite |
| title_full_unstemmed | Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite |
| title_short | Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite |
| title_sort | serum protein electrophoretic pattern in neonatal calves treated with clinoptilolite |
| topic | electrophoresis calves clinoptilolite γ-globulin |
| url | http://www.mdpi.com/1420-3049/23/6/1278 |
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