Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania
Introduction Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most st...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Rural Health
2017-12-01
|
| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/Total-phenolic-content-FTIR-analysis-and-antiproliferative-evaluation-of-lupin-seeds,80795,0,2.html |
| _version_ | 1818316702997282816 |
|---|---|
| author | Corina Danciu Ioana Zinuca Pavel Roxana Babuta Alexa Ersilia Suciu Oana Georgeta Pop Codruta Soica Cristina Dehelean Isidora Radulov |
| author_facet | Corina Danciu Ioana Zinuca Pavel Roxana Babuta Alexa Ersilia Suciu Oana Georgeta Pop Codruta Soica Cristina Dehelean Isidora Radulov |
| author_sort | Corina Danciu |
| collection | DOAJ |
| description | Introduction
Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most studied species due to their intense culinary use and potential biological activity. The intention of the study was to characterize total phenolic content, perform FTIR analysis, and antiproliferative effects against A375 human melanoma cells extracts obtained from germinated and ungerminated seeds from Lupinus albus L. and Lupinus angustifolius L.
Material and methods
Total phenolic content was assessed using the Folin-Ciocalteu colorimetric method. FTIR spectra were carried out by a Shimadzu Prestige-21 spectrometer in the range 400–4000 cm-1, using KBr pellets and resolution of 4 cm-1. Antiproliferative capacity was screened by employing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and scratch assay methods.
Results
The study showed increased values corresponding to total phenolic content for the germinated extracts. FTIR spectra confirmed the presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids). All tested extracts showed weak antiproliferative potential against A375 human melanoma cells.
Conclusions
Germination increased the amount of bioactive compounds in the seeds of the two studied species of lupin. FTIR analyses provided an important fingerprint of the chemical composition. |
| first_indexed | 2024-12-13T09:25:38Z |
| format | Article |
| id | doaj.art-1e119644cabd4410b070664caff96094 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-13T09:25:38Z |
| publishDate | 2017-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-1e119644cabd4410b070664caff960942022-12-21T23:52:37ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632017-12-0124472673110.26444/aaem/8079580795Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western RomaniaCorina Danciu0Ioana Zinuca Pavel1Roxana Babuta2Alexa Ersilia3Suciu Oana4Georgeta Pop5Codruta Soica6Cristina Dehelean7Isidora Radulov8University of Medicine and Pharmacy “Victor Babeş”, Timişoara, RomaniaUniversity of Medicine and Pharmacy “Victor Babeş”, Timişoara, RomaniaUniversity of Medicine and Pharmacy “Victor Babeş”, Timişoara, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, RomaniaDepartment of Microbiology, Discipline of Hygiene, Faculty of Pharmacy, University of Medicine and Pharmacy “Victor Babeş“, Timişoara, RomâniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, RomaniaUniversity of Medicine and Pharmacy “Victor Babeş”, Timişoara, RomaniaUniversity of Medicine and Pharmacy “Victor Babeş”, Timişoara, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, RomaniaIntroduction Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most studied species due to their intense culinary use and potential biological activity. The intention of the study was to characterize total phenolic content, perform FTIR analysis, and antiproliferative effects against A375 human melanoma cells extracts obtained from germinated and ungerminated seeds from Lupinus albus L. and Lupinus angustifolius L. Material and methods Total phenolic content was assessed using the Folin-Ciocalteu colorimetric method. FTIR spectra were carried out by a Shimadzu Prestige-21 spectrometer in the range 400–4000 cm-1, using KBr pellets and resolution of 4 cm-1. Antiproliferative capacity was screened by employing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and scratch assay methods. Results The study showed increased values corresponding to total phenolic content for the germinated extracts. FTIR spectra confirmed the presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids). All tested extracts showed weak antiproliferative potential against A375 human melanoma cells. Conclusions Germination increased the amount of bioactive compounds in the seeds of the two studied species of lupin. FTIR analyses provided an important fingerprint of the chemical composition.http://www.journalssystem.com/aaem/Total-phenolic-content-FTIR-analysis-and-antiproliferative-evaluation-of-lupin-seeds,80795,0,2.htmlantiproliferativeFTIRgerminationpolyphenolslupin seeds |
| spellingShingle | Corina Danciu Ioana Zinuca Pavel Roxana Babuta Alexa Ersilia Suciu Oana Georgeta Pop Codruta Soica Cristina Dehelean Isidora Radulov Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania Annals of Agricultural and Environmental Medicine antiproliferative FTIR germination polyphenols lupin seeds |
| title | Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania |
| title_full | Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania |
| title_fullStr | Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania |
| title_full_unstemmed | Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania |
| title_short | Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania |
| title_sort | total phenolic content ftir analysis and antiproliferative evaluation of lupin seeds harvest from western romania |
| topic | antiproliferative FTIR germination polyphenols lupin seeds |
| url | http://www.journalssystem.com/aaem/Total-phenolic-content-FTIR-analysis-and-antiproliferative-evaluation-of-lupin-seeds,80795,0,2.html |
| work_keys_str_mv | AT corinadanciu totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT ioanazinucapavel totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT roxanababuta totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT alexaersilia totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT suciuoana totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT georgetapop totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT codrutasoica totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT cristinadehelean totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania AT isidoraradulov totalphenoliccontentftiranalysisandantiproliferativeevaluationoflupinseedsharvestfromwesternromania |