Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study
The aim of the current study was to assess the multifaceted effects of the polycylic aromatic hydrocarbon phenanthrene, mainly used in the colouring, explosive, and pharmaceutical industries, on the physiology of two bivalve species with economic value as seafood, namely, the Mediterranean mussel &l...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/151 |
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| author | Mohamed Dellali Khadija Mardassi Abdel Halim Harrath Lamjed Mansour Octavian Pacioglu Waleed Aldahmash Saber Nahdi Riadh Badraoui Abdulwahed Fahad Alrefaei Fehmi Boufahja |
| author_facet | Mohamed Dellali Khadija Mardassi Abdel Halim Harrath Lamjed Mansour Octavian Pacioglu Waleed Aldahmash Saber Nahdi Riadh Badraoui Abdulwahed Fahad Alrefaei Fehmi Boufahja |
| author_sort | Mohamed Dellali |
| collection | DOAJ |
| description | The aim of the current study was to assess the multifaceted effects of the polycylic aromatic hydrocarbon phenanthrene, mainly used in the colouring, explosive, and pharmaceutical industries, on the physiology of two bivalve species with economic value as seafood, namely, the Mediterranean mussel <i>Mytilus galloprovincyalis</i> and the European clam <i>Ruditapes decussatus</i>. The current study assessed how the phenanthrene affected several biomarkers and biometric endpoints in both bivalves, based on an in vivo experiment in silico approach. The bivalves were exposed during four time slots (i.e., 7, 15, 21, and 28 days) to two concentrations of phenanthrene in water (50 µg/L and 100 µg/L). For the clam <i>R. decussatus</i>, an additional contamination of sediment was applied due their typical benthic lifestyle (50 µg/kg and 100 µg/kg). The phenanthrene significantly reduced the ability of bivalves to tolerate desiccation and their Median Lethal Time, and also inhibited the activity of the enzyme acetylcholinesterase in a time-dependent manner. The activity of catalase indicated that bivalves also experienced oxidative stress during the first 21 days of the experiment. The significant decline in catalase activity observed during the last week of the experiment for the mussel <i>M. galloprovincyalis</i> supported a depletion of enzymes caused by the phenanthrene. The phenanthrene has also toxicokinetic and toxicodynamic properties, as assessed by the in silico approach. Overall, the results obtained suggest that the bivalves <i>Ruditapes decussatus</i> and <i>M. galloprovincyalis</i> can be used as a sentinel species in monitoring studies to assess the environmental impact of phenanthene in marine ecosystems. The significance of our findings is based on the fact that in ecotoxicology, little is known about the chronic effects, the simultaneous use of multiple species as bioindicators, and the interactions molecular modelling. |
| first_indexed | 2024-03-11T10:08:37Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:08:37Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-1e12f82425944bf980e7a7a999e07a502023-11-16T14:48:48ZengMDPI AGAnimals2076-26152022-12-0113115110.3390/ani13010151Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers StudyMohamed Dellali0Khadija Mardassi1Abdel Halim Harrath2Lamjed Mansour3Octavian Pacioglu4Waleed Aldahmash5Saber Nahdi6Riadh Badraoui7Abdulwahed Fahad Alrefaei8Fehmi Boufahja9Coastal Ecology and Ecotoxicology Unit, LR01ES14 Laboratory of Environment Biomonitoring, Faculty of Sciences of Bizerte, University of Carthage, Tunis 7021, TunisiaCoastal Ecology and Ecotoxicology Unit, LR01ES14 Laboratory of Environment Biomonitoring, Faculty of Sciences of Bizerte, University of Carthage, Tunis 7021, TunisiaZoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaZoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaNational Institute of Research and Development for Biological Sciences, 060031 Bucharest, RomaniaZoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaZoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biology, Laboratory of General Biology, University of Ha’il, P.O. Box 2440, Ha’il 81451, Saudi ArabiaZoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box 90950, Riyadh 11623, Saudi ArabiaThe aim of the current study was to assess the multifaceted effects of the polycylic aromatic hydrocarbon phenanthrene, mainly used in the colouring, explosive, and pharmaceutical industries, on the physiology of two bivalve species with economic value as seafood, namely, the Mediterranean mussel <i>Mytilus galloprovincyalis</i> and the European clam <i>Ruditapes decussatus</i>. The current study assessed how the phenanthrene affected several biomarkers and biometric endpoints in both bivalves, based on an in vivo experiment in silico approach. The bivalves were exposed during four time slots (i.e., 7, 15, 21, and 28 days) to two concentrations of phenanthrene in water (50 µg/L and 100 µg/L). For the clam <i>R. decussatus</i>, an additional contamination of sediment was applied due their typical benthic lifestyle (50 µg/kg and 100 µg/kg). The phenanthrene significantly reduced the ability of bivalves to tolerate desiccation and their Median Lethal Time, and also inhibited the activity of the enzyme acetylcholinesterase in a time-dependent manner. The activity of catalase indicated that bivalves also experienced oxidative stress during the first 21 days of the experiment. The significant decline in catalase activity observed during the last week of the experiment for the mussel <i>M. galloprovincyalis</i> supported a depletion of enzymes caused by the phenanthrene. The phenanthrene has also toxicokinetic and toxicodynamic properties, as assessed by the in silico approach. Overall, the results obtained suggest that the bivalves <i>Ruditapes decussatus</i> and <i>M. galloprovincyalis</i> can be used as a sentinel species in monitoring studies to assess the environmental impact of phenanthene in marine ecosystems. The significance of our findings is based on the fact that in ecotoxicology, little is known about the chronic effects, the simultaneous use of multiple species as bioindicators, and the interactions molecular modelling.https://www.mdpi.com/2076-2615/13/1/151polycyclic aromatic hydrocarbonschronic toxicitybivalves<i>stress on stress</i>biomarkersin silico modelling |
| spellingShingle | Mohamed Dellali Khadija Mardassi Abdel Halim Harrath Lamjed Mansour Octavian Pacioglu Waleed Aldahmash Saber Nahdi Riadh Badraoui Abdulwahed Fahad Alrefaei Fehmi Boufahja Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study Animals polycyclic aromatic hydrocarbons chronic toxicity bivalves <i>stress on stress</i> biomarkers in silico modelling |
| title | Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study |
| title_full | Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study |
| title_fullStr | Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study |
| title_full_unstemmed | Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study |
| title_short | Physiological Responses of the Bivalves <i>Mytilus galloprovincialis</i> and <i>Ruditapes decussatus</i> Following Exposure to Phenanthrene: Toxicokinetics, Dynamics and Biomarkers Study |
| title_sort | physiological responses of the bivalves i mytilus galloprovincialis i and i ruditapes decussatus i following exposure to phenanthrene toxicokinetics dynamics and biomarkers study |
| topic | polycyclic aromatic hydrocarbons chronic toxicity bivalves <i>stress on stress</i> biomarkers in silico modelling |
| url | https://www.mdpi.com/2076-2615/13/1/151 |
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