Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions
Introduction: The microarchitecture of the mucosal and submucosal vessels is crucial for diagnosis of vocal fold lesions. Neo-angiogenesis is a confirmed biological parameter that implicates progression and metastasis in laryngeal cancer. Objective: This study investigates the correlation between va...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869419300941 |
| _version_ | 1819298000661381120 |
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| author | Anna Rzepakowska Michał Żurek Jakub Grzybowski Paweł Pihowicz Barbara Górnicka Ewa Osuch-Wójcikiewicz Kazimierz Niemczyk |
| author_facet | Anna Rzepakowska Michał Żurek Jakub Grzybowski Paweł Pihowicz Barbara Górnicka Ewa Osuch-Wójcikiewicz Kazimierz Niemczyk |
| author_sort | Anna Rzepakowska |
| collection | DOAJ |
| description | Introduction: The microarchitecture of the mucosal and submucosal vessels is crucial for diagnosis of vocal fold lesions. Neo-angiogenesis is a confirmed biological parameter that implicates progression and metastasis in laryngeal cancer. Objective: This study investigates the correlation between vascular pattern classifications by narrow band imaging and immunohistological microvessel density in different types of intraepithelial vocal fold lesions. Methods: Analysis of immunohistological microvessel density using CD31 and CD34 antibodies was performed in 77 lesions including: 20 non-dysplastic lesions, 20 with low-grade dysplasia, 17 with high-grade dysplasia and 20 invasive cancers. The evaluation of vascular patterns with narrow band imaging according to the Ni classification and European Laryngological Society guidelines was performed prior to surgical resection. Results: The mean value of CD31 microvessel density was the highest for Ni Type IV lesions (20.55), whereas for the longitudinal and perpendicular patterns according to the European Laryngological Society classification it was 12.50 and 19.45 respectively. The highest mean value of microvessel density with CD 34 was identified in Ni Type Va (35.43) lesions and in the longitudinal and perpendicular patterns according to the European Laryngological Society classification was 15.12 and 30.40 respectively. Conclusions: The microvascular morphological changes of intraepithelial laryngeal lesions observed under narrow band imaging endoscopy are positively correlated with angiogenesis indexes of immunohistological evaluation. |
| first_indexed | 2024-12-24T05:22:57Z |
| format | Article |
| id | doaj.art-1e1649f64f674eeabaf5d9e787808d99 |
| institution | Directory Open Access Journal |
| issn | 1808-8694 |
| language | English |
| last_indexed | 2024-12-24T05:22:57Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj.art-1e1649f64f674eeabaf5d9e787808d992022-12-21T17:13:24ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942021-03-01872137144Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesionsAnna Rzepakowska0Michał Żurek1Jakub Grzybowski2Paweł Pihowicz3Barbara Górnicka4Ewa Osuch-Wójcikiewicz5Kazimierz Niemczyk6Medical University of Warsaw, Otolaryngology Department, Warszawa, Poland; Corresponding author.Medical University of Warsaw, Otolaryngology Department, Warszawa, PolandMedical University of Warsaw, Department of Pathology, Warszawa, PolandMedical University of Warsaw, Department of Pathology, Warszawa, PolandMedical University of Warsaw, Department of Pathology, Warszawa, PolandMedical University of Warsaw, Otolaryngology Department, Warszawa, PolandMedical University of Warsaw, Otolaryngology Department, Warszawa, PolandIntroduction: The microarchitecture of the mucosal and submucosal vessels is crucial for diagnosis of vocal fold lesions. Neo-angiogenesis is a confirmed biological parameter that implicates progression and metastasis in laryngeal cancer. Objective: This study investigates the correlation between vascular pattern classifications by narrow band imaging and immunohistological microvessel density in different types of intraepithelial vocal fold lesions. Methods: Analysis of immunohistological microvessel density using CD31 and CD34 antibodies was performed in 77 lesions including: 20 non-dysplastic lesions, 20 with low-grade dysplasia, 17 with high-grade dysplasia and 20 invasive cancers. The evaluation of vascular patterns with narrow band imaging according to the Ni classification and European Laryngological Society guidelines was performed prior to surgical resection. Results: The mean value of CD31 microvessel density was the highest for Ni Type IV lesions (20.55), whereas for the longitudinal and perpendicular patterns according to the European Laryngological Society classification it was 12.50 and 19.45 respectively. The highest mean value of microvessel density with CD 34 was identified in Ni Type Va (35.43) lesions and in the longitudinal and perpendicular patterns according to the European Laryngological Society classification was 15.12 and 30.40 respectively. Conclusions: The microvascular morphological changes of intraepithelial laryngeal lesions observed under narrow band imaging endoscopy are positively correlated with angiogenesis indexes of immunohistological evaluation.http://www.sciencedirect.com/science/article/pii/S1808869419300941Intraepithelial lesionDysplasiaEarly glottis cancerMicrovessel densityNarrow band imaging |
| spellingShingle | Anna Rzepakowska Michał Żurek Jakub Grzybowski Paweł Pihowicz Barbara Górnicka Ewa Osuch-Wójcikiewicz Kazimierz Niemczyk Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions Brazilian Journal of Otorhinolaryngology Intraepithelial lesion Dysplasia Early glottis cancer Microvessel density Narrow band imaging |
| title | Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| title_full | Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| title_fullStr | Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| title_full_unstemmed | Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| title_short | Correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| title_sort | correlation of narrow band imaging vascular patterns with immunohistological microvessel density in vocal fold lesions |
| topic | Intraepithelial lesion Dysplasia Early glottis cancer Microvessel density Narrow band imaging |
| url | http://www.sciencedirect.com/science/article/pii/S1808869419300941 |
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