Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts
<i>Fusarium circinatum</i> poses a serious threat to many pine species in both commercial and natural pine forests. Knowledge regarding the molecular basis of pine-<i>F. circinatum</i> host-pathogen interactions could assist efforts to produce more resistant planting stock. T...
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MDPI AG
2019-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/9/315 |
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| author | Erik A. Visser Jill L. Wegrzyn Emma T. Steenkamp Alexander A. Myburg Sanushka Naidoo |
| author_facet | Erik A. Visser Jill L. Wegrzyn Emma T. Steenkamp Alexander A. Myburg Sanushka Naidoo |
| author_sort | Erik A. Visser |
| collection | DOAJ |
| description | <i>Fusarium circinatum</i> poses a serious threat to many pine species in both commercial and natural pine forests. Knowledge regarding the molecular basis of pine-<i>F. circinatum</i> host-pathogen interactions could assist efforts to produce more resistant planting stock. This study aimed to identify molecular responses underlying resistance against <i>F. circinatum</i>. A dual RNA-seq approach was used to investigate host and pathogen expression in <i>F. circinatum</i> challenged <i>Pinus tecunumanii</i> (resistant) and <i>Pinus patula</i> (susceptible), at three- and seven-days post inoculation. RNA-seq reads were mapped to combined host-pathogen references for both pine species to identify differentially expressed genes (DEGs). <i>F. circinatum</i> genes expressed during infection showed decreased ergosterol biosynthesis in <i>P. tecunumanii</i> relative to <i>P. patula</i>. For <i>P. tecunumanii</i>, enriched gene ontologies and DEGs indicated roles for auxin-, ethylene-, jasmonate- and salicylate-mediated phytohormone signalling. Correspondingly, key phytohormone signaling components were down-regulated in <i>P. patula</i>. Key <i>F. circinatum</i> ergosterol biosynthesis genes were expressed at lower levels during infection of the resistant relative to the susceptible host. This study further suggests that coordination of phytohormone signaling is required for <i>F. circinatum</i> resistance in <i>P. tecunumanii</i>, while a comparatively delayed response and impaired phytohormone signaling contributes to susceptibility in <i>P. patula</i>. |
| first_indexed | 2024-12-10T09:17:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-10T09:17:36Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1e17f5dbaed2496fa55af6839717eb4b2022-12-22T01:54:47ZengMDPI AGMicroorganisms2076-26072019-09-017931510.3390/microorganisms7090315microorganisms7090315Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) HostsErik A. Visser0Jill L. Wegrzyn1Emma T. Steenkamp2Alexander A. Myburg3Sanushka Naidoo4Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South AfricaDepartment of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USADepartment of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South AfricaDepartment of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South AfricaDepartment of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa<i>Fusarium circinatum</i> poses a serious threat to many pine species in both commercial and natural pine forests. Knowledge regarding the molecular basis of pine-<i>F. circinatum</i> host-pathogen interactions could assist efforts to produce more resistant planting stock. This study aimed to identify molecular responses underlying resistance against <i>F. circinatum</i>. A dual RNA-seq approach was used to investigate host and pathogen expression in <i>F. circinatum</i> challenged <i>Pinus tecunumanii</i> (resistant) and <i>Pinus patula</i> (susceptible), at three- and seven-days post inoculation. RNA-seq reads were mapped to combined host-pathogen references for both pine species to identify differentially expressed genes (DEGs). <i>F. circinatum</i> genes expressed during infection showed decreased ergosterol biosynthesis in <i>P. tecunumanii</i> relative to <i>P. patula</i>. For <i>P. tecunumanii</i>, enriched gene ontologies and DEGs indicated roles for auxin-, ethylene-, jasmonate- and salicylate-mediated phytohormone signalling. Correspondingly, key phytohormone signaling components were down-regulated in <i>P. patula</i>. Key <i>F. circinatum</i> ergosterol biosynthesis genes were expressed at lower levels during infection of the resistant relative to the susceptible host. This study further suggests that coordination of phytohormone signaling is required for <i>F. circinatum</i> resistance in <i>P. tecunumanii</i>, while a comparatively delayed response and impaired phytohormone signaling contributes to susceptibility in <i>P. patula</i>.https://www.mdpi.com/2076-2607/7/9/315Dual RNA-seqergosterol biosynthesis<i>Fusarium circinatum</i>host-pathogen interaction<i>Pinus patula</i><i>Pinus tecunumanii</i> |
| spellingShingle | Erik A. Visser Jill L. Wegrzyn Emma T. Steenkamp Alexander A. Myburg Sanushka Naidoo Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts Microorganisms Dual RNA-seq ergosterol biosynthesis <i>Fusarium circinatum</i> host-pathogen interaction <i>Pinus patula</i> <i>Pinus tecunumanii</i> |
| title | Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts |
| title_full | Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts |
| title_fullStr | Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts |
| title_full_unstemmed | Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts |
| title_short | Dual RNA-Seq Analysis of the Pine-<i>Fusarium circinatum</i> Interaction in Resistant (<i>Pinus tecunumanii</i>) and Susceptible (<i>Pinus patula</i>) Hosts |
| title_sort | dual rna seq analysis of the pine i fusarium circinatum i interaction in resistant i pinus tecunumanii i and susceptible i pinus patula i hosts |
| topic | Dual RNA-seq ergosterol biosynthesis <i>Fusarium circinatum</i> host-pathogen interaction <i>Pinus patula</i> <i>Pinus tecunumanii</i> |
| url | https://www.mdpi.com/2076-2607/7/9/315 |
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