Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation
The fructus of Kadsura coccinea known as 'Bu-Fu-Na' is an emerging internet-famous fruit. For deep processing of 'Bu-Fu-Na' related products, an approach combining LC-MS analysis, network pharmacological prediction, and experimental validation was adopted to explore phytochemical...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623000932 |
| _version_ | 1797869812209156096 |
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| author | Zhi-Ling Jin Ke Han Hao-Yang Chen Xiang-Yun Zhang Wei-Li Qiao Bei-Xi Jia |
| author_facet | Zhi-Ling Jin Ke Han Hao-Yang Chen Xiang-Yun Zhang Wei-Li Qiao Bei-Xi Jia |
| author_sort | Zhi-Ling Jin |
| collection | DOAJ |
| description | The fructus of Kadsura coccinea known as 'Bu-Fu-Na' is an emerging internet-famous fruit. For deep processing of 'Bu-Fu-Na' related products, an approach combining LC-MS analysis, network pharmacological prediction, and experimental validation was adopted to explore phytochemicals and biological functions of K. coccinea pericarpium (KCP) for the first time. In total, 9 lignans and 11 triterpenoids in KCP chloroform extract were identified by HPLC-Q-TOF/MS, and these chemicals acted on their key targets through multiple signaling pathways to achieve various biofunctions according to the constructed compound-target-pathway-disease network. Further experimental validation indicated that the four high-content lanostane-type triterpenoid acids in KCP strongly decreased 4T1 breast cancer cell viability in vitro, and the KCP chloroform extract had a good safety profile in 4T1 tumor-bearing mice and achieved anti-tumor efficacy by modulating immunity and activating the MAPK signal pathway. The obtained results provided comprehensive phytochemical and bioactive information for developing high value-added products of 'Bu-Fu-Na'. |
| first_indexed | 2024-04-10T00:17:38Z |
| format | Article |
| id | doaj.art-1e1b4bd351fa47fa83139e489def8e89 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-10T00:17:38Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-1e1b4bd351fa47fa83139e489def8e892023-03-16T05:03:36ZengElsevierJournal of Functional Foods1756-46462023-04-01103105493Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validationZhi-Ling Jin0Ke Han1Hao-Yang Chen2Xiang-Yun Zhang3Wei-Li Qiao4Bei-Xi Jia5Department of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaDepartment of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaDepartment of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaDepartment of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaDepartment of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaCorresponding author.; Department of Pharmacognosy, School of Pharmaceutical Science, Zhengzhou University, No. 100 of Science Road, Zhengzhou 450001, PR ChinaThe fructus of Kadsura coccinea known as 'Bu-Fu-Na' is an emerging internet-famous fruit. For deep processing of 'Bu-Fu-Na' related products, an approach combining LC-MS analysis, network pharmacological prediction, and experimental validation was adopted to explore phytochemicals and biological functions of K. coccinea pericarpium (KCP) for the first time. In total, 9 lignans and 11 triterpenoids in KCP chloroform extract were identified by HPLC-Q-TOF/MS, and these chemicals acted on their key targets through multiple signaling pathways to achieve various biofunctions according to the constructed compound-target-pathway-disease network. Further experimental validation indicated that the four high-content lanostane-type triterpenoid acids in KCP strongly decreased 4T1 breast cancer cell viability in vitro, and the KCP chloroform extract had a good safety profile in 4T1 tumor-bearing mice and achieved anti-tumor efficacy by modulating immunity and activating the MAPK signal pathway. The obtained results provided comprehensive phytochemical and bioactive information for developing high value-added products of 'Bu-Fu-Na'.http://www.sciencedirect.com/science/article/pii/S1756464623000932Kadsura coccinea fruitPhytochemical profileBioactive functions4T1 breast cancer cellTumor-bearing miceLanostane-type triterpenoid acid |
| spellingShingle | Zhi-Ling Jin Ke Han Hao-Yang Chen Xiang-Yun Zhang Wei-Li Qiao Bei-Xi Jia Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation Journal of Functional Foods Kadsura coccinea fruit Phytochemical profile Bioactive functions 4T1 breast cancer cell Tumor-bearing mice Lanostane-type triterpenoid acid |
| title | Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation |
| title_full | Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation |
| title_fullStr | Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation |
| title_full_unstemmed | Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation |
| title_short | Exploration of phytochemicals and biological functions of Kadsura coccinea pericarpium based on LC-MS and network pharmacology analysis and experimental validation |
| title_sort | exploration of phytochemicals and biological functions of kadsura coccinea pericarpium based on lc ms and network pharmacology analysis and experimental validation |
| topic | Kadsura coccinea fruit Phytochemical profile Bioactive functions 4T1 breast cancer cell Tumor-bearing mice Lanostane-type triterpenoid acid |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623000932 |
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