Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment
Abstract Background COVID-19 has emerged as the most serious outbreak in recent years. Certain medications such as sofosbuvir (SOF), ledipasvir (LDV) and paracetamol (PAR) were proposed as a safer and recommended substance to control symptoms of COVID-19. Results Using built-in spectrophotometer sof...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-08-01
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| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43094-023-00519-8 |
| _version_ | 1797453594995195904 |
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| author | Sherif Gamal Asmaa A. Mandour Gehad G. Mohamed Said A. Salih Dina A. Ahmed |
| author_facet | Sherif Gamal Asmaa A. Mandour Gehad G. Mohamed Said A. Salih Dina A. Ahmed |
| author_sort | Sherif Gamal |
| collection | DOAJ |
| description | Abstract Background COVID-19 has emerged as the most serious outbreak in recent years. Certain medications such as sofosbuvir (SOF), ledipasvir (LDV) and paracetamol (PAR) were proposed as a safer and recommended substance to control symptoms of COVID-19. Results Using built-in spectrophotometer software, zero order and derivative spectra of the studied components, two extremely clear, quick, and sensitive spectrophotometric techniques for simultaneous determinations of SOF, LDV, as well as PAR have been developed. LDV was calculated using a zero order absorption spectrum at wavelength maxima of 333 nm. SOF and PAR were evaluated simultaneously using a first derivative spectrophotometer at 247.2 nm and 260.8 nm, respectively. The calibration graphs for SOF, LDV, and PAR are linear over ranges of concentrations of 8–60 µg/mL, 4–22 µg/mL, and 2–14 µg/mL, consequently. The suggested methodologyspecificity was investigated using laboratory manufactured (different ratios) mixtures, which were then effectively used to the analysis of Mpiviropack plus® and Panadol® pills. Valid limitations included accuracy, precision, and specificity. The methodologies were validated in accordance with some ICH standards. Conclusions The methods proposed were simple, accurate, precise, and neither require any complex equipment nor specific software. |
| first_indexed | 2024-03-09T15:25:04Z |
| format | Article |
| id | doaj.art-1e1d92bc1cd54fd99ba28158463e7e1f |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-03-09T15:25:04Z |
| publishDate | 2023-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-1e1d92bc1cd54fd99ba28158463e7e1f2023-11-26T12:33:45ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532023-08-019111010.1186/s43094-023-00519-8Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatmentSherif Gamal0Asmaa A. Mandour1Gehad G. Mohamed2Said A. Salih3Dina A. Ahmed4Research Labs Supervisor, Faculty of Pharmacy, Future University in Egypt (FUE)Pharmaceutical Chemistry Department, Faculty of Pharmacy, Future University in Egypt (FUE)Chemistry Department, Faculty of Science, Cairo UniversityChemistry Department, Faculty of Science, Cairo UniversityPharmaceutical Chemistry Department, Faculty of Pharmacy, Future University in Egypt (FUE)Abstract Background COVID-19 has emerged as the most serious outbreak in recent years. Certain medications such as sofosbuvir (SOF), ledipasvir (LDV) and paracetamol (PAR) were proposed as a safer and recommended substance to control symptoms of COVID-19. Results Using built-in spectrophotometer software, zero order and derivative spectra of the studied components, two extremely clear, quick, and sensitive spectrophotometric techniques for simultaneous determinations of SOF, LDV, as well as PAR have been developed. LDV was calculated using a zero order absorption spectrum at wavelength maxima of 333 nm. SOF and PAR were evaluated simultaneously using a first derivative spectrophotometer at 247.2 nm and 260.8 nm, respectively. The calibration graphs for SOF, LDV, and PAR are linear over ranges of concentrations of 8–60 µg/mL, 4–22 µg/mL, and 2–14 µg/mL, consequently. The suggested methodologyspecificity was investigated using laboratory manufactured (different ratios) mixtures, which were then effectively used to the analysis of Mpiviropack plus® and Panadol® pills. Valid limitations included accuracy, precision, and specificity. The methodologies were validated in accordance with some ICH standards. Conclusions The methods proposed were simple, accurate, precise, and neither require any complex equipment nor specific software.https://doi.org/10.1186/s43094-023-00519-8SofosbuvirLedipasvirParacetamolCOVID-19Spectrophotometry |
| spellingShingle | Sherif Gamal Asmaa A. Mandour Gehad G. Mohamed Said A. Salih Dina A. Ahmed Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment Future Journal of Pharmaceutical Sciences Sofosbuvir Ledipasvir Paracetamol COVID-19 Spectrophotometry |
| title | Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment |
| title_full | Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment |
| title_fullStr | Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment |
| title_full_unstemmed | Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment |
| title_short | Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment |
| title_sort | simultaneous spectrophotometric determination of recombined sofosbuvir ledipasvir and paracetamol together as commonly repurposed drugs for covid 19 treatment |
| topic | Sofosbuvir Ledipasvir Paracetamol COVID-19 Spectrophotometry |
| url | https://doi.org/10.1186/s43094-023-00519-8 |
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