15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue
Background/Aims: Cyclooxygenase-2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (15-PGDH), and microsomal prostaglandin E synthase-1 (mPGEs-1) regulate prostaglandin E₂ (PGE₂) expression and are involved in colon carcinogenesis. We investigated the expression of PGE₂ and its regulating genes in spo...
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| Format: | Article |
| Language: | English |
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Korean Association for the Study of Intestinal Diseases
2017-01-01
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| Series: | Intestinal Research |
| Subjects: | |
| Online Access: | https://doi.org/10.5217/ir.2017.15.1.75 |
| _version_ | 1818298429795729408 |
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| author | Dong-Hoon Yang Yeon-Mi Ryu Sun-Mi Lee Jin-Yong Jeong Soon Man Yoon Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Seung-Jae Myung |
| author_facet | Dong-Hoon Yang Yeon-Mi Ryu Sun-Mi Lee Jin-Yong Jeong Soon Man Yoon Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Seung-Jae Myung |
| author_sort | Dong-Hoon Yang |
| collection | DOAJ |
| description | Background/Aims: Cyclooxygenase-2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (15-PGDH), and microsomal prostaglandin E synthase-1 (mPGEs-1) regulate prostaglandin E₂ (PGE₂) expression and are involved in colon carcinogenesis. We investigated the expression of PGE₂ and its regulating genes in sporadic human colon tumors and matched normal tissues.Methods: Twenty colonic adenomas and 27 colonic adenocarcinomas were evaluated. COX-2 and 15-PGDH expression was quantified by real-time polymerase chain reaction. The expression of PGE₂ and mPGEs-1 was measured using enzyme-linked immunosorbent assay and Western blotting, respectively.Results: The expression of COX-2, mPGEs-1, and PGE₂ did not differ between the adenomas and matched distant normal tissues. 15-PGDH expression was lower in adenomas than in the matched normal colonic tissues (P<0.001). In adenocarcinomas, mPGEs-1 and PGE₂ expression was significantly higher (P<0.001 and P=0.020, respectively), and COX-2 expression did not differ from that in normal tissues (P=0.207). 15-PGDH expression was significantly lower in the normal colonic mucosa from adenocarcinoma patients than in the normal mucosa from adenoma patients (P=0.018).Conclusions: Early inactivation of 15-PGDH, followed by activation of COX-2 and mPGEs-1, contributes to PGE₂ production, leading to colon carcinogenesis. 15-PGDH might be a novel candidate marker for early detection of field defects in colon carcinogenesis. |
| first_indexed | 2024-12-13T04:35:12Z |
| format | Article |
| id | doaj.art-1e1d9953bc0143549e1408756e83b825 |
| institution | Directory Open Access Journal |
| issn | 1598-9100 |
| language | English |
| last_indexed | 2024-12-13T04:35:12Z |
| publishDate | 2017-01-01 |
| publisher | Korean Association for the Study of Intestinal Diseases |
| record_format | Article |
| series | Intestinal Research |
| spelling | doaj.art-1e1d9953bc0143549e1408756e83b8252022-12-21T23:59:27ZengKorean Association for the Study of Intestinal DiseasesIntestinal Research1598-91002017-01-01151758210.5217/ir.2017.15.1.75ir.2017.15.1.7515-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissueDong-Hoon Yang0Yeon-Mi Ryu1Sun-Mi Lee2Jin-Yong Jeong3Soon Man Yoon4Byong Duk Ye5Jeong-Sik Byeon6Suk-Kyun Yang7Seung-Jae Myung8Department of Gastroenteroloy, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Internal Medicine, Chungbuk National University College of Medicine, Cheongju, Korea.Department of Gastroenteroloy, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Gastroenteroloy, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Gastroenteroloy, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Gastroenteroloy, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Background/Aims: Cyclooxygenase-2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (15-PGDH), and microsomal prostaglandin E synthase-1 (mPGEs-1) regulate prostaglandin E₂ (PGE₂) expression and are involved in colon carcinogenesis. We investigated the expression of PGE₂ and its regulating genes in sporadic human colon tumors and matched normal tissues.Methods: Twenty colonic adenomas and 27 colonic adenocarcinomas were evaluated. COX-2 and 15-PGDH expression was quantified by real-time polymerase chain reaction. The expression of PGE₂ and mPGEs-1 was measured using enzyme-linked immunosorbent assay and Western blotting, respectively.Results: The expression of COX-2, mPGEs-1, and PGE₂ did not differ between the adenomas and matched distant normal tissues. 15-PGDH expression was lower in adenomas than in the matched normal colonic tissues (P<0.001). In adenocarcinomas, mPGEs-1 and PGE₂ expression was significantly higher (P<0.001 and P=0.020, respectively), and COX-2 expression did not differ from that in normal tissues (P=0.207). 15-PGDH expression was significantly lower in the normal colonic mucosa from adenocarcinoma patients than in the normal mucosa from adenoma patients (P=0.018).Conclusions: Early inactivation of 15-PGDH, followed by activation of COX-2 and mPGEs-1, contributes to PGE₂ production, leading to colon carcinogenesis. 15-PGDH might be a novel candidate marker for early detection of field defects in colon carcinogenesis.https://doi.org/10.5217/ir.2017.15.1.7515-Hydroxyprostaglandin dehydrogenaseColonCarcinogenesis |
| spellingShingle | Dong-Hoon Yang Yeon-Mi Ryu Sun-Mi Lee Jin-Yong Jeong Soon Man Yoon Byong Duk Ye Jeong-Sik Byeon Suk-Kyun Yang Seung-Jae Myung 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue Intestinal Research 15-Hydroxyprostaglandin dehydrogenase Colon Carcinogenesis |
| title | 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue |
| title_full | 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue |
| title_fullStr | 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue |
| title_full_unstemmed | 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue |
| title_short | 15-Hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis: analysis of the prostaglandin pathway in human colonic tissue |
| title_sort | 15 hydroxyprostaglandin dehydrogenase as a marker in colon carcinogenesis analysis of the prostaglandin pathway in human colonic tissue |
| topic | 15-Hydroxyprostaglandin dehydrogenase Colon Carcinogenesis |
| url | https://doi.org/10.5217/ir.2017.15.1.75 |
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