A model for the RecA protein of Mycoplasma synoviae
In this work, we predict a structural model for the RecA protein from M. synoviae (MsRecA) by theoretical homology modeling and evaluate the occurrence of polymorphisms in this protein within several isolates of this species. The structural model suggested for MsRecA conserves the main domains prese...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2007-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200018 |
| _version_ | 1818524780530237440 |
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| author | Marbella Maria Fonseca Frank J.B. Alarcon Ana Tereza de Vasconcelos Lucymara Fassarela Agnez-Lima |
| author_facet | Marbella Maria Fonseca Frank J.B. Alarcon Ana Tereza de Vasconcelos Lucymara Fassarela Agnez-Lima |
| author_sort | Marbella Maria Fonseca |
| collection | DOAJ |
| description | In this work, we predict a structural model for the RecA protein from M. synoviae (MsRecA) by theoretical homology modeling and evaluate the occurrence of polymorphisms in this protein within several isolates of this species. The structural model suggested for MsRecA conserves the main domains present in MtRecA and EcRecA. The L1 and L2 regions showed six and three amino acid substitutions, respectively, which apparently do not affect the conformation and function of MsRecA. The C-terminal domain is shorter than that found in EcRecA and MtRecA, which may increase its capacity to bind dsDNA and displace SSB, compensating the absence of recombination initiation enzymes. The MS59 isolate RecA sequence showed one polymorphism which does not affect its functions since these belong to the same physical-chemical group. |
| first_indexed | 2024-12-11T06:01:21Z |
| format | Article |
| id | doaj.art-1e1dd79bd9f749178e903dc604eee042 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T06:01:21Z |
| publishDate | 2007-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-1e1dd79bd9f749178e903dc604eee0422022-12-22T01:18:27ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130129029510.1590/S1415-47572007000200018A model for the RecA protein of Mycoplasma synoviaeMarbella Maria FonsecaFrank J.B. AlarconAna Tereza de VasconcelosLucymara Fassarela Agnez-LimaIn this work, we predict a structural model for the RecA protein from M. synoviae (MsRecA) by theoretical homology modeling and evaluate the occurrence of polymorphisms in this protein within several isolates of this species. The structural model suggested for MsRecA conserves the main domains present in MtRecA and EcRecA. The L1 and L2 regions showed six and three amino acid substitutions, respectively, which apparently do not affect the conformation and function of MsRecA. The C-terminal domain is shorter than that found in EcRecA and MtRecA, which may increase its capacity to bind dsDNA and displace SSB, compensating the absence of recombination initiation enzymes. The MS59 isolate RecA sequence showed one polymorphism which does not affect its functions since these belong to the same physical-chemical group.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200018DNA repairrecombinationRecAMycoplasma synoviae |
| spellingShingle | Marbella Maria Fonseca Frank J.B. Alarcon Ana Tereza de Vasconcelos Lucymara Fassarela Agnez-Lima A model for the RecA protein of Mycoplasma synoviae Genetics and Molecular Biology DNA repair recombination RecA Mycoplasma synoviae |
| title | A model for the RecA protein of Mycoplasma synoviae |
| title_full | A model for the RecA protein of Mycoplasma synoviae |
| title_fullStr | A model for the RecA protein of Mycoplasma synoviae |
| title_full_unstemmed | A model for the RecA protein of Mycoplasma synoviae |
| title_short | A model for the RecA protein of Mycoplasma synoviae |
| title_sort | model for the reca protein of mycoplasma synoviae |
| topic | DNA repair recombination RecA Mycoplasma synoviae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200018 |
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