Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry
Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2023-1091 |
| _version_ | 1797646289660280832 |
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| author | Rottmann Jonas Schierbaum Nicolas Netaev Alexander Ligges Manuel Seidl Karsten |
| author_facet | Rottmann Jonas Schierbaum Nicolas Netaev Alexander Ligges Manuel Seidl Karsten |
| author_sort | Rottmann Jonas |
| collection | DOAJ |
| description | Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the application of this method. We summarize the requirements for a suited detector for time resolved flow cytometry (TRFC) and present a new developed SPAD array detector with 100 pixels and 340 ns time between measurement windows, allowing a high-throughput of up to 60,000 cells/s. The functionality of the detector is shown with the measurement of a 22.2 ns laser pulse, making it applicable for an improved TRFC. |
| first_indexed | 2024-03-11T15:00:28Z |
| format | Article |
| id | doaj.art-1e1e118dd137425da0d1a815252b63c8 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-03-11T15:00:28Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-1e1e118dd137425da0d1a815252b63c82023-10-30T07:58:12ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042023-09-019136336610.1515/cdbme-2023-1091Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometryRottmann Jonas0Schierbaum Nicolas1Netaev Alexander2Ligges Manuel3Seidl Karsten4Fraunhofer Institute for Microelectronic Circuits and Systems IMS, Finkenstraße 61, Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems IMS, Finkenstraße 61, Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems IMS, Finkenstraße 61, Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems IMS, Finkenstraße 61, Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems IMS, Finkenstraße 61, Duisburg, GermanyTime-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the application of this method. We summarize the requirements for a suited detector for time resolved flow cytometry (TRFC) and present a new developed SPAD array detector with 100 pixels and 340 ns time between measurement windows, allowing a high-throughput of up to 60,000 cells/s. The functionality of the detector is shown with the measurement of a 22.2 ns laser pulse, making it applicable for an improved TRFC.https://doi.org/10.1515/cdbme-2023-1091flow cytometryfluorescence lifetimespad array detector |
| spellingShingle | Rottmann Jonas Schierbaum Nicolas Netaev Alexander Ligges Manuel Seidl Karsten Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry Current Directions in Biomedical Engineering flow cytometry fluorescence lifetime spad array detector |
| title | Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry |
| title_full | Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry |
| title_fullStr | Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry |
| title_full_unstemmed | Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry |
| title_short | Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry |
| title_sort | single photon avalanche diode spad array detector for high throughput fluorescence lifetime flow cytometry |
| topic | flow cytometry fluorescence lifetime spad array detector |
| url | https://doi.org/10.1515/cdbme-2023-1091 |
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