Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hipp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.915907/full |
| _version_ | 1817986790765625344 |
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| author | Panagiotis Giannos Panagiotis Giannos Konstantinos Prokopidis Konstantinos Prokopidis |
| author_facet | Panagiotis Giannos Panagiotis Giannos Konstantinos Prokopidis Konstantinos Prokopidis |
| author_sort | Panagiotis Giannos |
| collection | DOAJ |
| description | Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hippocampal aging, remain scarce. Our study integrated gene expression profiles of the hippocampus from young and aged rats and functionally classified network-mapped genes based on their interactome. Hippocampal differentially expressed genes (DEGs) between young (5–8 months) and aged (21–26 months) male rats (Rattus norvegicus) were retrieved from five publicly available datasets (GSE14505, GSE20219, GSE14723, GSE14724, and GSE14725; 38 young and 29 aged samples). Encoded hippocampal proteins of age-related DEGs and their interactome were predicted. Clustered network DEGs were identified and the highest-ranked was functionally annotated. A single cluster of 19 age-related hippocampal DEGs was revealed, which was linked with immune response (biological process, P = 1.71E-17), immunoglobulin G binding (molecular function, P = 1.92E-08), and intrinsic component of plasma membrane (cellular component, P = 1.25E-06). Our findings revealed dysregulated hippocampal immunoglobulin dynamics in the aging rat brain. Whether a consequence of neurovascular perturbations and dysregulated blood-brain barrier permeability, the role of hippocampal immunoregulation in the pathobiology of aging warrants further investigation. |
| first_indexed | 2024-04-14T00:13:59Z |
| format | Article |
| id | doaj.art-1e210905e5b742a8a2a275c7392cc7ec |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-14T00:13:59Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-1e210905e5b742a8a2a275c7392cc7ec2022-12-22T02:23:13ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-05-011610.3389/fnins.2022.915907915907Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin DynamicsPanagiotis Giannos0Panagiotis Giannos1Konstantinos Prokopidis2Konstantinos Prokopidis3Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomSociety of Meta-Research and Biomedical Innovation, London, United KingdomSociety of Meta-Research and Biomedical Innovation, London, United KingdomDepartment of Musculoskeletal Biology, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, United KingdomAging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hippocampal aging, remain scarce. Our study integrated gene expression profiles of the hippocampus from young and aged rats and functionally classified network-mapped genes based on their interactome. Hippocampal differentially expressed genes (DEGs) between young (5–8 months) and aged (21–26 months) male rats (Rattus norvegicus) were retrieved from five publicly available datasets (GSE14505, GSE20219, GSE14723, GSE14724, and GSE14725; 38 young and 29 aged samples). Encoded hippocampal proteins of age-related DEGs and their interactome were predicted. Clustered network DEGs were identified and the highest-ranked was functionally annotated. A single cluster of 19 age-related hippocampal DEGs was revealed, which was linked with immune response (biological process, P = 1.71E-17), immunoglobulin G binding (molecular function, P = 1.92E-08), and intrinsic component of plasma membrane (cellular component, P = 1.25E-06). Our findings revealed dysregulated hippocampal immunoglobulin dynamics in the aging rat brain. Whether a consequence of neurovascular perturbations and dysregulated blood-brain barrier permeability, the role of hippocampal immunoregulation in the pathobiology of aging warrants further investigation.https://www.frontiersin.org/articles/10.3389/fnins.2022.915907/fullhippocampusagingrat braindifferentially expressed genesgene expressionimmunoglobulins |
| spellingShingle | Panagiotis Giannos Panagiotis Giannos Konstantinos Prokopidis Konstantinos Prokopidis Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics Frontiers in Neuroscience hippocampus aging rat brain differentially expressed genes gene expression immunoglobulins |
| title | Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics |
| title_full | Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics |
| title_fullStr | Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics |
| title_full_unstemmed | Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics |
| title_short | Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics |
| title_sort | gene expression profiles of the aging rat hippocampus imply altered immunoglobulin dynamics |
| topic | hippocampus aging rat brain differentially expressed genes gene expression immunoglobulins |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.915907/full |
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