The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder
Introduction Autism spectrum disorder (ASD) is a multi-factorial disease characterized by impairments in social interaction, communication and repetitive behaviors. The necessity of developing an adequate treatment for ASD is essential. There is an increase in clinical studies assessing the positi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2021-04-01
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| Series: | European Psychiatry |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S0924933821005484/type/journal_article |
| _version_ | 1797616559020048384 |
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| author | M.A. Robea A. Ciobica S.-A. Strungaru G. Plavan M. Nicoara C. Solcan |
| author_facet | M.A. Robea A. Ciobica S.-A. Strungaru G. Plavan M. Nicoara C. Solcan |
| author_sort | M.A. Robea |
| collection | DOAJ |
| description |
Introduction
Autism spectrum disorder (ASD) is a multi-factorial disease characterized by impairments in social interaction, communication and repetitive behaviors. The necessity of developing an adequate treatment for ASD is essential. There is an increase in clinical studies assessing the positive effects of vitamins in ASD children. Vitamin C (vit. C) is implicated in biosynthesis of neurotransmitters and in protein metabolism.
Objectives
This study evaluated the possible effect of vit. C on zebrafish sociability after a single insecticide mixture administration as inductor for ASD.
Methods
A single dose of insecticide mixture (600 μg L-1 fipronil and 600 μg L-1 pyriproxyfen) was administrated to zebrafish juvenile. Vit. C (25 μg L−1) was daily administrated during 14 days. A control group simulated the administration of insecticide mixture and vitamin. Each animal was tested in the experimental tank designed for the social interaction test. The trials were recorded and analysed using EthoVision XT 11 (NOLDUS, Netherlands). The locomotor activity parameters and the time spent next to the group were measured. Each trial had 4 minutes duration.
Results
We have found no significant differences in the average levels between pre-treatment and treatment days (P< 0.05 ANOVA) regarding the locomotor activity parameters. Significant changes in sociability were observed for the group exposed to insecticide mixture and for vit. C group (P > 0.05 ANOVA). It was also found that 14 days vitamin administration can lead to sociability improvements after a single administration of mixture insecticide.
Conclusions
The results of the current study bring some positive insights for the future of ASD therapy.
Conflict of interest
This work was co-funded by the European Social Fund, through Operational Programme Human Capital 2014-2020, project number POCU/380/6/13/123623, project title
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| first_indexed | 2024-03-11T07:41:52Z |
| format | Article |
| id | doaj.art-1e224f7f06f24e839b9148dd9f5f5cab |
| institution | Directory Open Access Journal |
| issn | 0924-9338 1778-3585 |
| language | English |
| last_indexed | 2024-03-11T07:41:52Z |
| publishDate | 2021-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | European Psychiatry |
| spelling | doaj.art-1e224f7f06f24e839b9148dd9f5f5cab2023-11-17T05:08:16ZengCambridge University PressEuropean Psychiatry0924-93381778-35852021-04-0164S206S20610.1192/j.eurpsy.2021.548The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorderM.A. Robea0A. Ciobica1S.-A. Strungaru2G. Plavan3M. Nicoara4C. Solcan5Department Of Biology, “Alexandru Ioan Cuza” University of Iasi, Faculty of Biology, Iasi, RomaniaDepartment Of Research, “Alexandru Ioan Cuza” University of Iasi, Faculty of Biology, Iasi, Romania Center Of Biomedical Research, Romanian Academy, Iasi, RomaniaDepartment Of Interdisciplinary Research In Science, “Alexandru Ioan Cuza” University of Iasi, Iasi, RomaniaDepartment Of Biology, “Alexandru Ioan Cuza” University of Iasi, Faculty of Biology, Iasi, RomaniaDepartment Of Biology, “Alexandru Ioan Cuza” University of Iasi, Faculty of Biology, Iasi, RomaniaDepartment Of Molecular Biology, Histology And Embriology, University of Agricultural Science and Veterinary Medicine “Ion Ionescu de la Brad”, Faculty of Veterinary Medicine, Iasi, Romania Introduction Autism spectrum disorder (ASD) is a multi-factorial disease characterized by impairments in social interaction, communication and repetitive behaviors. The necessity of developing an adequate treatment for ASD is essential. There is an increase in clinical studies assessing the positive effects of vitamins in ASD children. Vitamin C (vit. C) is implicated in biosynthesis of neurotransmitters and in protein metabolism. Objectives This study evaluated the possible effect of vit. C on zebrafish sociability after a single insecticide mixture administration as inductor for ASD. Methods A single dose of insecticide mixture (600 μg L-1 fipronil and 600 μg L-1 pyriproxyfen) was administrated to zebrafish juvenile. Vit. C (25 μg L−1) was daily administrated during 14 days. A control group simulated the administration of insecticide mixture and vitamin. Each animal was tested in the experimental tank designed for the social interaction test. The trials were recorded and analysed using EthoVision XT 11 (NOLDUS, Netherlands). The locomotor activity parameters and the time spent next to the group were measured. Each trial had 4 minutes duration. Results We have found no significant differences in the average levels between pre-treatment and treatment days (P< 0.05 ANOVA) regarding the locomotor activity parameters. Significant changes in sociability were observed for the group exposed to insecticide mixture and for vit. C group (P > 0.05 ANOVA). It was also found that 14 days vitamin administration can lead to sociability improvements after a single administration of mixture insecticide. Conclusions The results of the current study bring some positive insights for the future of ASD therapy. Conflict of interest This work was co-funded by the European Social Fund, through Operational Programme Human Capital 2014-2020, project number POCU/380/6/13/123623, project title https://www.cambridge.org/core/product/identifier/S0924933821005484/type/journal_articlevitamin Cpesticideautism spectrum disordersociability |
| spellingShingle | M.A. Robea A. Ciobica S.-A. Strungaru G. Plavan M. Nicoara C. Solcan The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder European Psychiatry vitamin C pesticide autism spectrum disorder sociability |
| title | The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder |
| title_full | The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder |
| title_fullStr | The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder |
| title_full_unstemmed | The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder |
| title_short | The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder |
| title_sort | effect of vitamin c on sociability in a juvenile zebrafish pesticide induced model of autism spectrum disorder |
| topic | vitamin C pesticide autism spectrum disorder sociability |
| url | https://www.cambridge.org/core/product/identifier/S0924933821005484/type/journal_article |
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