The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation
Ionizing radiation is a potentially chromosomal damaging agent. The induction of chromosomal damage as well as the incidence of cell cycle disturbances may depend on the dose of irradiation. One of the indication of chromosomal damage is the formation of micronucleus (MN) during the anaphase of mit...
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| Format: | Article |
| Language: | English |
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Center for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN)
2005-07-01
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| Series: | Atom Indonesia |
| Subjects: | |
| Online Access: | http://aij.batan.go.id/index.php/aij/article/view/365 |
| _version_ | 1818546758906544128 |
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| author | R. Sofyan Y. Sumpena M. Lukita A. Fitrisari |
| author_facet | R. Sofyan Y. Sumpena M. Lukita A. Fitrisari |
| author_sort | R. Sofyan |
| collection | DOAJ |
| description | Ionizing radiation is a potentially chromosomal damaging agent. The induction of chromosomal damage as well as the incidence of cell cycle disturbances may depend on the dose of irradiation. One of the indication of chromosomal damage is the formation of micronucleus (MN) during the anaphase of mitosis. This study deals with the MN assay on femur bone marrow polychromatic erythrocyte (PCE) cells of Swiss-Webster mice, for the mutagenicity test of g-irradiation. The study was conducted on five groups of mice (each group consist of five mice) that were irradiated at the doses of 0; 0,2; 0,4; 0,6 and 0,8 Gy respectively. One day after irradiation, the mice were killed by cervical dislocation. Furthermore the femur bone marrow was taken, the cells were then prepared by smear technique onto slides followed by Giemsa staining. The MN in PCE cells or MNPCE were examined microscopically by the magnification of 1000 and counted for every 1000 cells in each mice. The results showed that the MNPCE frequencies on the treatment groups were significantly higher than that of the control (P< 0,05). Further evaluation indicated that the MNPCE frequencies increased with the increase of irradiation dose. |
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| id | doaj.art-1e229a27859f4bc9b248fa88e0d7c86e |
| institution | Directory Open Access Journal |
| issn | 0126-1568 0126-1568 |
| language | English |
| last_indexed | 2024-12-12T07:57:31Z |
| publishDate | 2005-07-01 |
| publisher | Center for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN) |
| record_format | Article |
| series | Atom Indonesia |
| spelling | doaj.art-1e229a27859f4bc9b248fa88e0d7c86e2022-12-22T00:32:16ZengCenter for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN)Atom Indonesia0126-15680126-15682005-07-01312103110http://dx.doi.org/10.17146/aij.2005.365The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-IrradiationR. Sofyan0Y. Sumpena1M. Lukita2A. Fitrisari3Center for Technology of Material and Radiometry, BATAN, IndonesiaDepartment of Biology, Padjadjaran University, Bandung, IndonesiaDepartment of Biology, Padjadjaran University, Bandung, IndonesiaDepartment of Biology, Indonesian University of Education, IndonesiaIonizing radiation is a potentially chromosomal damaging agent. The induction of chromosomal damage as well as the incidence of cell cycle disturbances may depend on the dose of irradiation. One of the indication of chromosomal damage is the formation of micronucleus (MN) during the anaphase of mitosis. This study deals with the MN assay on femur bone marrow polychromatic erythrocyte (PCE) cells of Swiss-Webster mice, for the mutagenicity test of g-irradiation. The study was conducted on five groups of mice (each group consist of five mice) that were irradiated at the doses of 0; 0,2; 0,4; 0,6 and 0,8 Gy respectively. One day after irradiation, the mice were killed by cervical dislocation. Furthermore the femur bone marrow was taken, the cells were then prepared by smear technique onto slides followed by Giemsa staining. The MN in PCE cells or MNPCE were examined microscopically by the magnification of 1000 and counted for every 1000 cells in each mice. The results showed that the MNPCE frequencies on the treatment groups were significantly higher than that of the control (P< 0,05). Further evaluation indicated that the MNPCE frequencies increased with the increase of irradiation dose.http://aij.batan.go.id/index.php/aij/article/view/365micronucleusbone marrowpolychromatic erythrocytegamma;-irradiationSwiss-Webster |
| spellingShingle | R. Sofyan Y. Sumpena M. Lukita A. Fitrisari The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation Atom Indonesia micronucleus bone marrow polychromatic erythrocyte gamma;-irradiation Swiss-Webster |
| title | The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation |
| title_full | The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation |
| title_fullStr | The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation |
| title_full_unstemmed | The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation |
| title_short | The use of Micronucleus Assay on Swiss-Webster Mice (Mus Musculus) Bone Marrow for the Mutagenicity Test of γ-Irradiation |
| title_sort | use of micronucleus assay on swiss webster mice mus musculus bone marrow for the mutagenicity test of γ irradiation |
| topic | micronucleus bone marrow polychromatic erythrocyte gamma;-irradiation Swiss-Webster |
| url | http://aij.batan.go.id/index.php/aij/article/view/365 |
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