Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates
Emergence of antimicrobial resistance complicates treatment of infections by antibiotics. This has driven research on novel and combination antibacterial therapies. The present study evaluated synergistic antimicrobial activity of plant extracts and cefixime in resistant clinical isolates. Prelimina...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Saudi Journal of Biological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X23000219 |
| _version_ | 1811157858248556544 |
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| author | Sania Atta Durdana Waseem Humaira Fatima Iffat Naz Faisal Rasheed Nosheen Kanwal |
| author_facet | Sania Atta Durdana Waseem Humaira Fatima Iffat Naz Faisal Rasheed Nosheen Kanwal |
| author_sort | Sania Atta |
| collection | DOAJ |
| description | Emergence of antimicrobial resistance complicates treatment of infections by antibiotics. This has driven research on novel and combination antibacterial therapies. The present study evaluated synergistic antimicrobial activity of plant extracts and cefixime in resistant clinical isolates. Preliminary susceptibility profiling of antibiotics and antibacterial activity of extracts was done by disc diffusion and microbroth dilution assays. Checker-board, time-kill kinetics and protein content studies were performed to validate synergistic antibacterial activity. Results showed noteworthy quantities of gallic acid (0.24–19.7 µg/mg), quercetin (1.57–18.44 µg/mg) and cinnamic acid (0.02–5.93 µg/mg) in extracts of plants assessed by reverse-phase high performance liquid chromatography (RP-HPLC). Gram-positive (4/6) and Gram-negative (13/16) clinical isolates were intermediately susceptible or resistant to cefixime, which was used for synergistic studies. EA and M extracts of plants exhibited total synergy, partial synergy and indifferent characteristics whereas aqueous extracts did not show synergistic patterns. Time-kill kinetic studies showed that synergism was both time and concentration-dependent (2–8-fold decrease in concentration). Bacterial isolates treated with combinations at fractional inhibitory concentration index (FICI) showed significantly reduced bacterial growth, as well as protein content (5–62 %) as compared to extracts/cefixime alone treated isolates. This study acknowledges the selected crude extracts as adjuvants to antibiotics to treat resistant bacterial infections. |
| first_indexed | 2024-04-10T05:14:33Z |
| format | Article |
| id | doaj.art-1e2e6735a69f44b28c74fec927c50eb4 |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-04-10T05:14:33Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-1e2e6735a69f44b28c74fec927c50eb42023-03-09T04:12:50ZengElsevierSaudi Journal of Biological Sciences1319-562X2023-03-01303103576Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolatesSania Atta0Durdana Waseem1Humaira Fatima2Iffat Naz3Faisal Rasheed4Nosheen Kanwal5Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, PakistanShifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Jaffer Khan Jamali Road, H-8/4, Islamabad, PakistanDepartment of Pharmacy, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biology, Science Unit, Deanship of Educational Services, Qassim University, Buraidah 51452, Saudi ArabiaPatients Diagnostic Lab, Isotope Application Division, Pakistan Institute of Nuclear Science And Technology (PINSTECH), Islamabad, PakistanDepartment of Biochemistry, Science Unit, Deanship of Educational Services, Qassim University, Buraidah 51452, Saudi Arabia; Corresponding author.Emergence of antimicrobial resistance complicates treatment of infections by antibiotics. This has driven research on novel and combination antibacterial therapies. The present study evaluated synergistic antimicrobial activity of plant extracts and cefixime in resistant clinical isolates. Preliminary susceptibility profiling of antibiotics and antibacterial activity of extracts was done by disc diffusion and microbroth dilution assays. Checker-board, time-kill kinetics and protein content studies were performed to validate synergistic antibacterial activity. Results showed noteworthy quantities of gallic acid (0.24–19.7 µg/mg), quercetin (1.57–18.44 µg/mg) and cinnamic acid (0.02–5.93 µg/mg) in extracts of plants assessed by reverse-phase high performance liquid chromatography (RP-HPLC). Gram-positive (4/6) and Gram-negative (13/16) clinical isolates were intermediately susceptible or resistant to cefixime, which was used for synergistic studies. EA and M extracts of plants exhibited total synergy, partial synergy and indifferent characteristics whereas aqueous extracts did not show synergistic patterns. Time-kill kinetic studies showed that synergism was both time and concentration-dependent (2–8-fold decrease in concentration). Bacterial isolates treated with combinations at fractional inhibitory concentration index (FICI) showed significantly reduced bacterial growth, as well as protein content (5–62 %) as compared to extracts/cefixime alone treated isolates. This study acknowledges the selected crude extracts as adjuvants to antibiotics to treat resistant bacterial infections.http://www.sciencedirect.com/science/article/pii/S1319562X23000219Antimicrobial resistanceMultidrug-resistanceAntibacterial activityTime-kill kineticsCrude extractsSynergism |
| spellingShingle | Sania Atta Durdana Waseem Humaira Fatima Iffat Naz Faisal Rasheed Nosheen Kanwal Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates Saudi Journal of Biological Sciences Antimicrobial resistance Multidrug-resistance Antibacterial activity Time-kill kinetics Crude extracts Synergism |
| title | Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| title_full | Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| title_fullStr | Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| title_full_unstemmed | Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| title_short | Antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| title_sort | antibacterial potential and synergistic interaction between natural polyphenolic extracts and synthetic antibiotic on clinical isolates |
| topic | Antimicrobial resistance Multidrug-resistance Antibacterial activity Time-kill kinetics Crude extracts Synergism |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X23000219 |
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