Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland
Abstract Pear (Pyrus spp.) is one of the most consumed fruits in China, but the pear production has to confront the growing threat from fatal diseases. In this study, we report two incidences of stem canker and twig dieback disease on pear plants, which led to death of pear seedlings (approximately...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Phytopathology Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42483-022-00111-7 |
| _version_ | 1811273473986658304 |
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| author | Feng He Jie Yang Yancun Zhao Pedro Laborda Yifan Jia Asma Safdar Alex Machio Kange Bingxin Li Lan Zhou Quan Zeng Sally Brown Zheng Qing Fu Fengquan Liu |
| author_facet | Feng He Jie Yang Yancun Zhao Pedro Laborda Yifan Jia Asma Safdar Alex Machio Kange Bingxin Li Lan Zhou Quan Zeng Sally Brown Zheng Qing Fu Fengquan Liu |
| author_sort | Feng He |
| collection | DOAJ |
| description | Abstract Pear (Pyrus spp.) is one of the most consumed fruits in China, but the pear production has to confront the growing threat from fatal diseases. In this study, we report two incidences of stem canker and twig dieback disease on pear plants, which led to death of pear seedlings (approximately 10% of total plants) in Guangxi and Jiangsu provinces. Using a combination of morphological and molecular diagnoses, along with pathogenicity test, the causal agent of the disease in these two locations was identified to be the fungus Neofusicoccum parvum. However, the isolates were divided into two clades: CY-2 isolate and other four isolates including ZL-4, BM-9, BM-10 and BM-12 might split into two groups of N. parvum. Two representative isolates (CY-2 and ZL-4) were selected for further investigation. We observed that the optimal temperature for in vitro infection on pear trees of these two isolates was at round 25 °C. Both CY-2 and ZL-4 could infect different sand pear varieties and other horticultural plants in vitro, while CY-2 had a higher virulence on several pear varieties including Nanyue, Lvyun, Qiushui and Ningmenghuang. Furthermore, the efficacy of fungicides against these two isolates was evaluated, and carbendazim and flusilazole were found to be the most effective fungicides in inhibiting the growth of these fungal pathogens. Taken together, these findings redefine the N. parvum species and provide potential strategies for the future management of this disease. |
| first_indexed | 2024-04-12T22:59:46Z |
| format | Article |
| id | doaj.art-1e30a7f9f05a4a418327e58d45f52b82 |
| institution | Directory Open Access Journal |
| issn | 2524-4167 |
| language | English |
| last_indexed | 2024-04-12T22:59:46Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Phytopathology Research |
| spelling | doaj.art-1e30a7f9f05a4a418327e58d45f52b822022-12-22T03:13:05ZengBMCPhytopathology Research2524-41672022-03-014111110.1186/s42483-022-00111-7Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainlandFeng He0Jie Yang1Yancun Zhao2Pedro Laborda3Yifan Jia4Asma Safdar5Alex Machio Kange6Bingxin Li7Lan Zhou8Quan Zeng9Sally Brown10Zheng Qing Fu11Fengquan Liu12Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyCollege of Life Sciences, Anhui Normal UniversityInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyAcademy of Agricultural Sciences of YanbianDepartment of Plant Pathology and Ecology, The Connecticut Agricultural Experiment StationDepartment of Biological Sciences, University of South CarolinaDepartment of Biological Sciences, University of South CarolinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyAbstract Pear (Pyrus spp.) is one of the most consumed fruits in China, but the pear production has to confront the growing threat from fatal diseases. In this study, we report two incidences of stem canker and twig dieback disease on pear plants, which led to death of pear seedlings (approximately 10% of total plants) in Guangxi and Jiangsu provinces. Using a combination of morphological and molecular diagnoses, along with pathogenicity test, the causal agent of the disease in these two locations was identified to be the fungus Neofusicoccum parvum. However, the isolates were divided into two clades: CY-2 isolate and other four isolates including ZL-4, BM-9, BM-10 and BM-12 might split into two groups of N. parvum. Two representative isolates (CY-2 and ZL-4) were selected for further investigation. We observed that the optimal temperature for in vitro infection on pear trees of these two isolates was at round 25 °C. Both CY-2 and ZL-4 could infect different sand pear varieties and other horticultural plants in vitro, while CY-2 had a higher virulence on several pear varieties including Nanyue, Lvyun, Qiushui and Ningmenghuang. Furthermore, the efficacy of fungicides against these two isolates was evaluated, and carbendazim and flusilazole were found to be the most effective fungicides in inhibiting the growth of these fungal pathogens. Taken together, these findings redefine the N. parvum species and provide potential strategies for the future management of this disease.https://doi.org/10.1186/s42483-022-00111-7PearNeofusicoccum parvumPathogenicitySusceptibility |
| spellingShingle | Feng He Jie Yang Yancun Zhao Pedro Laborda Yifan Jia Asma Safdar Alex Machio Kange Bingxin Li Lan Zhou Quan Zeng Sally Brown Zheng Qing Fu Fengquan Liu Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland Phytopathology Research Pear Neofusicoccum parvum Pathogenicity Susceptibility |
| title | Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland |
| title_full | Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland |
| title_fullStr | Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland |
| title_full_unstemmed | Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland |
| title_short | Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland |
| title_sort | identification and characterization of a stem canker and twig dieback disease of pear caused by neofusicoccum parvum in chinese mainland |
| topic | Pear Neofusicoccum parvum Pathogenicity Susceptibility |
| url | https://doi.org/10.1186/s42483-022-00111-7 |
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