Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft.
The objective of the present study was to perform an in vivo assessment of a novel silk-collagen scaffold for anterior cruciate ligament (ACL) reconstruction. First, a silk-collagen scaffold was fabricated by combining sericin-extracted knitted silk fibroin mesh and type I collagen to mimic the comp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4418759?pdf=render |
| _version_ | 1818233399753572352 |
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| author | Fanggang Bi Zhongli Shi An Liu Peng Guo Shigui Yan |
| author_facet | Fanggang Bi Zhongli Shi An Liu Peng Guo Shigui Yan |
| author_sort | Fanggang Bi |
| collection | DOAJ |
| description | The objective of the present study was to perform an in vivo assessment of a novel silk-collagen scaffold for anterior cruciate ligament (ACL) reconstruction. First, a silk-collagen scaffold was fabricated by combining sericin-extracted knitted silk fibroin mesh and type I collagen to mimic the components of the ligament. Scaffolds were electron-beam sterilized and rolled up to replace the ACL in 20 rabbits in the scaffold group, and autologous semitendinosus tendons were used to reconstruct the ACL in the autograft control group. At 4 and 16 weeks after surgery, grafts were retrieved and analyzed for neoligament regeneration and tendon-bone healing. To evaluate neoligament regeneration, H&E and immunohistochemical staining was performed, and to assess tendon-bone healing, micro-CT, biomechanical test, H&E and Russell-Movat pentachrome staining were performed. Cell infiltration increased over time in the scaffold group, and abundant fibroblast-like cells were found in the core of the scaffold graft at 16 weeks postoperatively. Tenascin-C was strongly positive in newly regenerated tissue at 4 and 16 weeks postoperatively in the scaffold group, similar to observations in the autograft group. Compared with the autograft group, tendon-bone healing was better in the scaffold group with trabecular bone growth into the scaffold. The results indicate that the silk-collagen scaffold has considerable potential for clinical application. |
| first_indexed | 2024-12-12T11:21:34Z |
| format | Article |
| id | doaj.art-1e3222db01e04efaa177796c0f66913a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T11:21:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1e3222db01e04efaa177796c0f66913a2022-12-22T00:26:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012590010.1371/journal.pone.0125900Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft.Fanggang BiZhongli ShiAn LiuPeng GuoShigui YanThe objective of the present study was to perform an in vivo assessment of a novel silk-collagen scaffold for anterior cruciate ligament (ACL) reconstruction. First, a silk-collagen scaffold was fabricated by combining sericin-extracted knitted silk fibroin mesh and type I collagen to mimic the components of the ligament. Scaffolds were electron-beam sterilized and rolled up to replace the ACL in 20 rabbits in the scaffold group, and autologous semitendinosus tendons were used to reconstruct the ACL in the autograft control group. At 4 and 16 weeks after surgery, grafts were retrieved and analyzed for neoligament regeneration and tendon-bone healing. To evaluate neoligament regeneration, H&E and immunohistochemical staining was performed, and to assess tendon-bone healing, micro-CT, biomechanical test, H&E and Russell-Movat pentachrome staining were performed. Cell infiltration increased over time in the scaffold group, and abundant fibroblast-like cells were found in the core of the scaffold graft at 16 weeks postoperatively. Tenascin-C was strongly positive in newly regenerated tissue at 4 and 16 weeks postoperatively in the scaffold group, similar to observations in the autograft group. Compared with the autograft group, tendon-bone healing was better in the scaffold group with trabecular bone growth into the scaffold. The results indicate that the silk-collagen scaffold has considerable potential for clinical application.http://europepmc.org/articles/PMC4418759?pdf=render |
| spellingShingle | Fanggang Bi Zhongli Shi An Liu Peng Guo Shigui Yan Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. PLoS ONE |
| title | Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. |
| title_full | Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. |
| title_fullStr | Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. |
| title_full_unstemmed | Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. |
| title_short | Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. |
| title_sort | anterior cruciate ligament reconstruction in a rabbit model using silk collagen scaffold and comparison with autograft |
| url | http://europepmc.org/articles/PMC4418759?pdf=render |
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