Expanding the Toolbox for Functional Genomics in <i>Fonsecaea pedrosoi</i>: The Use of Split-Marker and Biolistic Transformation for Inactivation of Tryptophan Synthase (<i>trpB</i>) Gene

Expanding the Toolbox for Functional Genomics in <i>Fonsecaea pedrosoi</i>: The Use of Split-Marker and Biolistic Transformation for Inactivation of Tryptophan Synthase (<i>trpB</i>) Gene

Chromoblastomycosis (CBM) is a disease caused by several dematiaceous fungi from different genera, and <i>Fonsecaea</i> is the most common which has been clinically isolated. Genetic transformation methods have recently been described; however, molecular tools for the functional study of...

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Bibliographic Details
Main Authors: Luísa Dan Favilla, Tatiana Sobianski Herman, Camila da Silva Goersch, Rosangela Vieira de Andrade, Maria Sueli Soares Felipe, Anamélia Lorenzetti Bocca, Larissa Fernandes
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Journal of Fungi
Subjects:
gene replacement by homologous recombination
double-joint PCR
biolistic transformation
knockout mutant
<i>trpB</i>
tryptophan biosynthesis
Online Access:https://www.mdpi.com/2309-608X/9/2/224

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