Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles
A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was depo...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/19/1/40 |
| _version_ | 1817990247463518208 |
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| author | Chenxing Guo Jianfang Wang Xianzhe Chen Yujiao Li Lifang Wu Jin Zhang Cheng-an Tao |
| author_facet | Chenxing Guo Jianfang Wang Xianzhe Chen Yujiao Li Lifang Wu Jin Zhang Cheng-an Tao |
| author_sort | Chenxing Guo |
| collection | DOAJ |
| description | A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability. |
| first_indexed | 2024-04-14T00:57:00Z |
| format | Article |
| id | doaj.art-1e36221da984405b8c9dc4f3eb78c42c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T00:57:00Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1e36221da984405b8c9dc4f3eb78c42c2022-12-22T02:21:34ZengMDPI AGSensors1424-82202018-12-011914010.3390/s19010040s19010040Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold NanoparticlesChenxing Guo0Jianfang Wang1Xianzhe Chen2Yujiao Li3Lifang Wu4Jin Zhang5Cheng-an Tao6Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, ChinaCollege of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, ChinaKey Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, ChinaKey Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, ChinaCollege of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, ChinaA biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability.http://www.mdpi.com/1424-8220/19/1/40biosensorcytochrome cgraphenegold nanoparticleshydrogen peroxidedirect electrochemistry |
| spellingShingle | Chenxing Guo Jianfang Wang Xianzhe Chen Yujiao Li Lifang Wu Jin Zhang Cheng-an Tao Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles Sensors biosensor cytochrome c graphene gold nanoparticles hydrogen peroxide direct electrochemistry |
| title | Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles |
| title_full | Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles |
| title_fullStr | Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles |
| title_full_unstemmed | Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles |
| title_short | Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles |
| title_sort | construction of a biosensor based on a combination of cytochrome c graphene and gold nanoparticles |
| topic | biosensor cytochrome c graphene gold nanoparticles hydrogen peroxide direct electrochemistry |
| url | http://www.mdpi.com/1424-8220/19/1/40 |
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