Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections
Objective This study aims to verify any antibacterial activity of honey against bacteria concerned with Respiratory Tract Infections in Karachi, Pakistan. Methodology Three brands of commercial honey, one rude and two processed were used to determine their antibacterial activity against four...
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| Format: | Article |
| Language: | English |
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Dow University of Health Sciences
2012-12-01
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| Series: | Journal of the Dow University of Health Sciences |
| Subjects: | |
| Online Access: | https://jduhs.com/index.php/jduhs/article/view/1485 |
| _version_ | 1797818411528486912 |
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| author | Farhan Essaa Abdullah Kiran Afab Rabia Khanum Sidrah Sohail Khan |
| author_facet | Farhan Essaa Abdullah Kiran Afab Rabia Khanum Sidrah Sohail Khan |
| author_sort | Farhan Essaa Abdullah |
| collection | DOAJ |
| description | Objective
This study aims to verify any antibacterial activity of honey against bacteria concerned with Respiratory Tract Infections in Karachi, Pakistan.
Methodology
Three brands of commercial honey, one rude and two processed were used to determine their antibacterial activity against four bacterial species, Klebsiella pneumoniae (n=56), Pneumococci (n=34), Pseudomonas aeruginosa (n=16), and Staphylococcus aureus (n=20), isolated from Sputum and Throat swab specimens. The cork-bore method was employed using Mueller-Hinton agar, and the inhibition zones around the wells containing 20%w/v honey solutions were assessed using Imipenem disc (30ug) as the standard of antibacterial activity. Data was analyzed using SPSS 16.0.
Results
Significant antibacterial activity of honey was observed against the 4 RTI isolates, with higher sensitivities for ProcS (Salman s Honey) and Crude sample. The Crude sample yielded relatively superior inhibition zones than the other two specimens; it affected all 34 (100%) of Pneumococci, 29 of 56 (52%) Klebsiellae, 14 of 20 (70%) Staphylococci and 4 of 16 (25%) Pseudomonas isolates. Pneumococci were significantly more sensitive to all 3 honey specimens, while pseudomonas were least sensitive compared to other isolates.
Conclusion
Samples of honey showed promising in vitro antibacterial activity on some RTI isolates.
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| first_indexed | 2024-03-13T09:07:33Z |
| format | Article |
| id | doaj.art-1e3817a1b2494e21bb7aaeb634e99005 |
| institution | Directory Open Access Journal |
| issn | 1995-2198 2410-2180 |
| language | English |
| last_indexed | 2024-03-13T09:07:33Z |
| publishDate | 2012-12-01 |
| publisher | Dow University of Health Sciences |
| record_format | Article |
| series | Journal of the Dow University of Health Sciences |
| spelling | doaj.art-1e3817a1b2494e21bb7aaeb634e990052023-05-28T03:11:15ZengDow University of Health SciencesJournal of the Dow University of Health Sciences1995-21982410-21802012-12-0163Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract InfectionsFarhan Essaa Abdullah0Kiran Afab1Rabia Khanum2Sidrah Sohail Khan3Dr. Essa’s Laboratory and Diagnostic Centre, Karachi, PakistanThird Year Student, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan.Third Year Student, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan.Third Year Student, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan.Objective This study aims to verify any antibacterial activity of honey against bacteria concerned with Respiratory Tract Infections in Karachi, Pakistan. Methodology Three brands of commercial honey, one rude and two processed were used to determine their antibacterial activity against four bacterial species, Klebsiella pneumoniae (n=56), Pneumococci (n=34), Pseudomonas aeruginosa (n=16), and Staphylococcus aureus (n=20), isolated from Sputum and Throat swab specimens. The cork-bore method was employed using Mueller-Hinton agar, and the inhibition zones around the wells containing 20%w/v honey solutions were assessed using Imipenem disc (30ug) as the standard of antibacterial activity. Data was analyzed using SPSS 16.0. Results Significant antibacterial activity of honey was observed against the 4 RTI isolates, with higher sensitivities for ProcS (Salman s Honey) and Crude sample. The Crude sample yielded relatively superior inhibition zones than the other two specimens; it affected all 34 (100%) of Pneumococci, 29 of 56 (52%) Klebsiellae, 14 of 20 (70%) Staphylococci and 4 of 16 (25%) Pseudomonas isolates. Pneumococci were significantly more sensitive to all 3 honey specimens, while pseudomonas were least sensitive compared to other isolates. Conclusion Samples of honey showed promising in vitro antibacterial activity on some RTI isolates. https://jduhs.com/index.php/jduhs/article/view/1485Honeyantibacterial sensitivityrespiratory infections |
| spellingShingle | Farhan Essaa Abdullah Kiran Afab Rabia Khanum Sidrah Sohail Khan Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections Journal of the Dow University of Health Sciences Honey antibacterial sensitivity respiratory infections |
| title | Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections |
| title_full | Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections |
| title_fullStr | Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections |
| title_full_unstemmed | Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections |
| title_short | Antibacterial Activity of Honey Against Bacteria Isolated from Respiratory Tract Infections |
| title_sort | antibacterial activity of honey against bacteria isolated from respiratory tract infections |
| topic | Honey antibacterial sensitivity respiratory infections |
| url | https://jduhs.com/index.php/jduhs/article/view/1485 |
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