Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity
Abstract Background Chemical reduction has become an accessible and useful alternative to obtain silver nanoparticles (AgNPs). However, its toxicity capacity depends on multiple variables that generate differences in the ability to inhibit the growth of microorganisms. Thus, optimazing parameters fo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2019-12-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40824-019-0173-y |
| _version_ | 1797281929565831168 |
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| author | Catalina Quintero-Quiroz Natalia Acevedo Jenniffer Zapata-Giraldo Luz E. Botero Julián Quintero Diana Zárate-Triviño Jorge Saldarriaga Vera Z. Pérez |
| author_facet | Catalina Quintero-Quiroz Natalia Acevedo Jenniffer Zapata-Giraldo Luz E. Botero Julián Quintero Diana Zárate-Triviño Jorge Saldarriaga Vera Z. Pérez |
| author_sort | Catalina Quintero-Quiroz |
| collection | DOAJ |
| description | Abstract Background Chemical reduction has become an accessible and useful alternative to obtain silver nanoparticles (AgNPs). However, its toxicity capacity depends on multiple variables that generate differences in the ability to inhibit the growth of microorganisms. Thus, optimazing parameters for the synthesis of AgNPs can increase its antimicrobial capacity by improving its physical-chemical properties. Methods In this study a Face Centered Central Composite Design (FCCCD) was carried out with four parameters: A g N O 3 concentration, sodium citrate (TSC) concentration, N a B H 4 concentration and the pH of the reaction with the objective of inhibit the growth of microorganisms. The response variables were the average size of AgNPs, the peak with the greatest intensity in the size distribution, the polydispersity of the nanoparticle size and the yield of the process. AgNPs obtained from the optimization were characterized physically and chemically. The antimicrobial activity of optimized AgNPs was evaluated against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans and compared with AgNPs before optimization. In addition, the cytotoxicity of the optimized AgNPs was evaluated by the colorimetric assay MTT (3- (4,5- Dimethylthiazol- 2- yl)- 2, 5 - Diphenyltetrazolium Bromide). Results It was found that the four factors studied were significant for the response variables, and a significant model (p < 0.05) was obtained for each variable. The optimal conditions were 8 for pH and 0.01 M, 0.0 6M, 0.01 M for the concentration of TSC, A g N O 3, and N a B H 4, respectively. Optimized AgNPs spherical and hemispherical were obtained, and 67.66% of it had a diameter less than 10.30 nm. A minimum bactericidal concentration (MBC) and minimum fungicidal Concentration (MFC) of optimized AgNPs was found against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans at 19.89, 9.94, 9.94, 2.08 μg/mL, respectively. Furthermore, the lethal concentration 50 (L C 50) of optimized AgNPs was found on 19.11 μg/mL and 19.60 μg/mL to Vero and NiH3T3 cells, respectively. Conclusions It was found that the factors studied were significant for the variable responses and the optimization process used was effective to improve the antimicrobial activity of the AgNPs. |
| first_indexed | 2024-03-07T17:04:33Z |
| format | Article |
| id | doaj.art-1e3cc59716974ffb8c6d4e10e0347683 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T17:04:33Z |
| publishDate | 2019-12-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-1e3cc59716974ffb8c6d4e10e03476832024-03-03T02:43:07ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242019-12-0123111510.1186/s40824-019-0173-yOptimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activityCatalina Quintero-Quiroz0Natalia Acevedo1Jenniffer Zapata-Giraldo2Luz E. Botero3Julián Quintero4Diana Zárate-Triviño5Jorge Saldarriaga6Vera Z. Pérez7Centro de Bioingeniería, Grupo de investigaciones en Bioingeniería, Universidad Pontificia BolivarianaCentro de Bioingeniería, Grupo de investigaciones en Bioingeniería, Universidad Pontificia BolivarianaGrupo de Investigación de Biología de Sistemas,Universidad Pontificia BolivarianaGrupo de Investigación de Biología de Sistemas,Universidad Pontificia BolivarianaUniversidad de AntioquiaLaboratorio de Inmunología y Virología, Universidad Autónoma de Nuevo LeónGrupo de Investigación Sobre Nuevos Materiales, Universidad Pontificia BolivarianaCentro de Bioingeniería, Grupo de investigaciones en Bioingeniería, Universidad Pontificia BolivarianaAbstract Background Chemical reduction has become an accessible and useful alternative to obtain silver nanoparticles (AgNPs). However, its toxicity capacity depends on multiple variables that generate differences in the ability to inhibit the growth of microorganisms. Thus, optimazing parameters for the synthesis of AgNPs can increase its antimicrobial capacity by improving its physical-chemical properties. Methods In this study a Face Centered Central Composite Design (FCCCD) was carried out with four parameters: A g N O 3 concentration, sodium citrate (TSC) concentration, N a B H 4 concentration and the pH of the reaction with the objective of inhibit the growth of microorganisms. The response variables were the average size of AgNPs, the peak with the greatest intensity in the size distribution, the polydispersity of the nanoparticle size and the yield of the process. AgNPs obtained from the optimization were characterized physically and chemically. The antimicrobial activity of optimized AgNPs was evaluated against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans and compared with AgNPs before optimization. In addition, the cytotoxicity of the optimized AgNPs was evaluated by the colorimetric assay MTT (3- (4,5- Dimethylthiazol- 2- yl)- 2, 5 - Diphenyltetrazolium Bromide). Results It was found that the four factors studied were significant for the response variables, and a significant model (p < 0.05) was obtained for each variable. The optimal conditions were 8 for pH and 0.01 M, 0.0 6M, 0.01 M for the concentration of TSC, A g N O 3, and N a B H 4, respectively. Optimized AgNPs spherical and hemispherical were obtained, and 67.66% of it had a diameter less than 10.30 nm. A minimum bactericidal concentration (MBC) and minimum fungicidal Concentration (MFC) of optimized AgNPs was found against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans at 19.89, 9.94, 9.94, 2.08 μg/mL, respectively. Furthermore, the lethal concentration 50 (L C 50) of optimized AgNPs was found on 19.11 μg/mL and 19.60 μg/mL to Vero and NiH3T3 cells, respectively. Conclusions It was found that the factors studied were significant for the variable responses and the optimization process used was effective to improve the antimicrobial activity of the AgNPs.https://doi.org/10.1186/s40824-019-0173-ySilver nanoparticlesDesign of experimentsResponse surface methodologyAntimicrobial activityCytotoxicity |
| spellingShingle | Catalina Quintero-Quiroz Natalia Acevedo Jenniffer Zapata-Giraldo Luz E. Botero Julián Quintero Diana Zárate-Triviño Jorge Saldarriaga Vera Z. Pérez Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity Biomaterials Research Silver nanoparticles Design of experiments Response surface methodology Antimicrobial activity Cytotoxicity |
| title | Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| title_full | Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| title_fullStr | Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| title_full_unstemmed | Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| title_short | Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| title_sort | optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity |
| topic | Silver nanoparticles Design of experiments Response surface methodology Antimicrobial activity Cytotoxicity |
| url | https://doi.org/10.1186/s40824-019-0173-y |
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