In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus
During the screening programme for antibiotic produced by actinomycetes, an antibiotic was isolated from the fermentation broth of Streptomyces anulatus NEAE-94. The ethyl acetate extract of S. anulatus NEAE-94 showed high biological activity against Staphylococcus aureus NRRL B-313, multidrug-resis...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-03-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1276412 |
| _version_ | 1818235162896367616 |
|---|---|
| author | Noura El-Ahmady El-Naggar Ashraf Abdal-Aziz El-Bindary Mamdouh Abdel-Mogib Noura Salah Nour |
| author_facet | Noura El-Ahmady El-Naggar Ashraf Abdal-Aziz El-Bindary Mamdouh Abdel-Mogib Noura Salah Nour |
| author_sort | Noura El-Ahmady El-Naggar |
| collection | DOAJ |
| description | During the screening programme for antibiotic produced by actinomycetes, an antibiotic was isolated from the fermentation broth of Streptomyces anulatus NEAE-94. The ethyl acetate extract of S. anulatus NEAE-94 showed high biological activity against Staphylococcus aureus NRRL B-313, multidrug-resistant S. aureus and Bacillus subtilis NRRL B-543. Thin-layer chromatography of the ethyl acetate extract showed one yellowish orange spot with an Rf value of 0.83. The structural elucidation of the isolated compounds was carried out using ultraviolet–visible mass spectrometry (MS), Fourier transform-infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS) analyses. Characterization of the ethyl acetate extract revealed the presence of 22 compounds, including an unsaturated fatty acid, saturated fatty acids, fatty acid esters, alkanes, alkenes and a triterpene. The antimicrobial efficacy of S. anulatus NEAE-94 might be due to the synergistic effects of the identified compounds. |
| first_indexed | 2024-12-12T11:49:36Z |
| format | Article |
| id | doaj.art-1e41cb1282d0480bba0b410edbd4c231 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-12T11:49:36Z |
| publishDate | 2017-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-1e41cb1282d0480bba0b410edbd4c2312022-12-22T00:25:21ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-03-0131241843010.1080/13102818.2016.12764121276412In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureusNoura El-Ahmady El-Naggar0Ashraf Abdal-Aziz El-Bindary1Mamdouh Abdel-Mogib2Noura Salah Nour3Genetic Engineering and Biotechnology Research InstituteDamietta UniversityMansoura UniversityGenetic Engineering and Biotechnology Research InstituteDuring the screening programme for antibiotic produced by actinomycetes, an antibiotic was isolated from the fermentation broth of Streptomyces anulatus NEAE-94. The ethyl acetate extract of S. anulatus NEAE-94 showed high biological activity against Staphylococcus aureus NRRL B-313, multidrug-resistant S. aureus and Bacillus subtilis NRRL B-543. Thin-layer chromatography of the ethyl acetate extract showed one yellowish orange spot with an Rf value of 0.83. The structural elucidation of the isolated compounds was carried out using ultraviolet–visible mass spectrometry (MS), Fourier transform-infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS) analyses. Characterization of the ethyl acetate extract revealed the presence of 22 compounds, including an unsaturated fatty acid, saturated fatty acids, fatty acid esters, alkanes, alkenes and a triterpene. The antimicrobial efficacy of S. anulatus NEAE-94 might be due to the synergistic effects of the identified compounds.http://dx.doi.org/10.1080/13102818.2016.1276412Streptomyces anulatusantibiotic1H-NMRFT-IRGC–MS analysismultidrug-resistant Staphylococcus aureus |
| spellingShingle | Noura El-Ahmady El-Naggar Ashraf Abdal-Aziz El-Bindary Mamdouh Abdel-Mogib Noura Salah Nour In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus Biotechnology & Biotechnological Equipment Streptomyces anulatus antibiotic 1H-NMR FT-IR GC–MS analysis multidrug-resistant Staphylococcus aureus |
| title | In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus |
| title_full | In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus |
| title_fullStr | In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus |
| title_full_unstemmed | In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus |
| title_short | In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus |
| title_sort | in vitro activity extraction separation and structure elucidation of antibiotic produced by streptomyces anulatus neae 94 active against multidrug resistant staphylococcus aureus |
| topic | Streptomyces anulatus antibiotic 1H-NMR FT-IR GC–MS analysis multidrug-resistant Staphylococcus aureus |
| url | http://dx.doi.org/10.1080/13102818.2016.1276412 |
| work_keys_str_mv | AT nouraelahmadyelnaggar invitroactivityextractionseparationandstructureelucidationofantibioticproducedbystreptomycesanulatusneae94activeagainstmultidrugresistantstaphylococcusaureus AT ashrafabdalazizelbindary invitroactivityextractionseparationandstructureelucidationofantibioticproducedbystreptomycesanulatusneae94activeagainstmultidrugresistantstaphylococcusaureus AT mamdouhabdelmogib invitroactivityextractionseparationandstructureelucidationofantibioticproducedbystreptomycesanulatusneae94activeagainstmultidrugresistantstaphylococcusaureus AT nourasalahnour invitroactivityextractionseparationandstructureelucidationofantibioticproducedbystreptomycesanulatusneae94activeagainstmultidrugresistantstaphylococcusaureus |