Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery

Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery

It is essential to explore the relationship between drugs and transporters in the process of drug development. Strong background signals in nonhuman MDCK or LLC-PK1 cells and overlapping interference of inhibitors or RNAi in human Caco-2 cells mean that an ideal alternative could be to knock out spe...

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Bibliographic Details
Main Authors: Dongyan Feng, Guorui Zhong, Qingxia Zuo, Yanbin Wan, Wanqing Xu, Changsheng He, Cailing Lin, Dongchao Huang, Feng Chen, Lizhen Huang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Pharmacology
Subjects:
transporter substrate identification
ABC efflux transporters
CRISPR/Cas9
gene knockout
Caco-2
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2022.1015940/full

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