Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury
Poor functional recovery after spinal cord injury (SCI) drives the development of novel strategies to manage this devastating condition. We recently showed promising immunomodulatory and pro-regenerative actions of bio-functionalized carbon microfibres (MFs) implanted in a rodent model of SCI. In or...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/6/839 |
| _version_ | 1827750772993949696 |
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| author | Vincent Escarrat Jimena Perez-Sanchez Bilal El-Waly Jorge E. Collazos-Castro Franck Debarbieux |
| author_facet | Vincent Escarrat Jimena Perez-Sanchez Bilal El-Waly Jorge E. Collazos-Castro Franck Debarbieux |
| author_sort | Vincent Escarrat |
| collection | DOAJ |
| description | Poor functional recovery after spinal cord injury (SCI) drives the development of novel strategies to manage this devastating condition. We recently showed promising immunomodulatory and pro-regenerative actions of bio-functionalized carbon microfibres (MFs) implanted in a rodent model of SCI. In order to maximize tissue repair while easing MF implantation, we produced a composite implant based on the embedding of several MFs within a fibrin hydrogel. We used intravital imaging of fluorescent reporter mice at the early stages and spinal sections of the same animals 3 months later to characterize the neuroinflammatory response to the implant and its impact on axonal regeneration. Whereas fibrin alone was inert in the first week, its enzymatic degradation drove the chronic activation of microglial cells and axonal degeneration within 3 months. However, the presence of MFs inside the fibrin hydrogel slowed down fibrin degradation and boosted the early recruitment of immune cells. Noteworthy, there was an enhanced contribution of monocyte-derived dendritic cells (moDCs), preceding a faster transition toward an anti-inflammatory environment with increased axonal regeneration over 3 months. The inclusion of MF here ensured the long-term biocompatibility of fibrin hydrogels, which would otherwise preclude successful spinal cord regeneration. |
| first_indexed | 2024-03-11T06:47:22Z |
| format | Article |
| id | doaj.art-1e435833429647a0bee915455210667a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T06:47:22Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1e435833429647a0bee915455210667a2023-11-17T10:12:34ZengMDPI AGCells2073-44092023-03-0112683910.3390/cells12060839Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord InjuryVincent Escarrat0Jimena Perez-Sanchez1Bilal El-Waly2Jorge E. Collazos-Castro3Franck Debarbieux4Institut des Neurosciences de la Timone, Aix-Marseille Université and CNRS UMR7289, 13005 Marseille, FranceInstitut des Neurosciences de la Timone, Aix-Marseille Université and CNRS UMR7289, 13005 Marseille, FranceInstitut des Neurosciences de la Timone, Aix-Marseille Université and CNRS UMR7289, 13005 Marseille, FranceNeural Repair and Biomaterials Laboratory, Hospital Nacional de Parapléjicos (SESCAM), 45071 Toledo, SpainInstitut des Neurosciences de la Timone, Aix-Marseille Université and CNRS UMR7289, 13005 Marseille, FrancePoor functional recovery after spinal cord injury (SCI) drives the development of novel strategies to manage this devastating condition. We recently showed promising immunomodulatory and pro-regenerative actions of bio-functionalized carbon microfibres (MFs) implanted in a rodent model of SCI. In order to maximize tissue repair while easing MF implantation, we produced a composite implant based on the embedding of several MFs within a fibrin hydrogel. We used intravital imaging of fluorescent reporter mice at the early stages and spinal sections of the same animals 3 months later to characterize the neuroinflammatory response to the implant and its impact on axonal regeneration. Whereas fibrin alone was inert in the first week, its enzymatic degradation drove the chronic activation of microglial cells and axonal degeneration within 3 months. However, the presence of MFs inside the fibrin hydrogel slowed down fibrin degradation and boosted the early recruitment of immune cells. Noteworthy, there was an enhanced contribution of monocyte-derived dendritic cells (moDCs), preceding a faster transition toward an anti-inflammatory environment with increased axonal regeneration over 3 months. The inclusion of MF here ensured the long-term biocompatibility of fibrin hydrogels, which would otherwise preclude successful spinal cord regeneration.https://www.mdpi.com/2073-4409/12/6/839two-photon microscopytransgenic fluorescent micebiocompatibilityneuroinflammationspinal cord injurynatural materials |
| spellingShingle | Vincent Escarrat Jimena Perez-Sanchez Bilal El-Waly Jorge E. Collazos-Castro Franck Debarbieux Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury Cells two-photon microscopy transgenic fluorescent mice biocompatibility neuroinflammation spinal cord injury natural materials |
| title | Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury |
| title_full | Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury |
| title_fullStr | Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury |
| title_full_unstemmed | Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury |
| title_short | Composite Fibrin and Carbon Microfibre Implant to Modulate Postraumatic Inflammation after Spinal Cord Injury |
| title_sort | composite fibrin and carbon microfibre implant to modulate postraumatic inflammation after spinal cord injury |
| topic | two-photon microscopy transgenic fluorescent mice biocompatibility neuroinflammation spinal cord injury natural materials |
| url | https://www.mdpi.com/2073-4409/12/6/839 |
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