Detection of Trypanosoma brucei by microwave cytometry
Researchers studying cellular life cycles need to be able to monitor the phases of a cell cycle rapidly and accurately. Many of the techniques currently used to monitor the cell cycle require the use of labels and would be difficult to automate. Microwave cytometry is a promising new approach to lab...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053922000017 |
| _version_ | 1828120675826532352 |
|---|---|
| author | Jeffrey A. Osterberg Jillian Milanes James Morris Pingshan Wang |
| author_facet | Jeffrey A. Osterberg Jillian Milanes James Morris Pingshan Wang |
| author_sort | Jeffrey A. Osterberg |
| collection | DOAJ |
| description | Researchers studying cellular life cycles need to be able to monitor the phases of a cell cycle rapidly and accurately. Many of the techniques currently used to monitor the cell cycle require the use of labels and would be difficult to automate. Microwave cytometry is a promising new approach to label free monitoring of cell life cycles. This paper presents results of multiple frequency microwave measurements of two lifecycle stages of Trypanosoma brucei, a unicellular eukaryotic parasite found in sub-Saharan Africa. A microwave flow cytometer was used to show bloodstream form (BSF) and procyclic form (PCF) T. brucei have frequency dependent permittivity and impedance from 800 MHz to 7.65 GHz. The two cell forms had a strong dependence on the imaginary part of permittivity at 2.38 GHz and below and a strong dependence on the real part of permittivity at 5.55 GHz and above. Three PCF cell lines were tested to verify that the differences between the two cell forms were independent of cell strain. Additionally, impedance measurements were used to improve cell classification in cases where the permittivity of a cell cannot be detected. Quadratic discriminate analysis was employed to validate the ability to classify cells forms, with maximum cross-validation errors of 15.4% and 10% when using one and three PCF strains, respectively. |
| first_indexed | 2024-04-11T14:08:53Z |
| format | Article |
| id | doaj.art-1e477cbf957d497dac0de8e29437e60c |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-04-11T14:08:53Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-1e477cbf957d497dac0de8e29437e60c2022-12-22T04:19:46ZengElsevierSensors and Actuators Reports2666-05392022-11-014100074Detection of Trypanosoma brucei by microwave cytometryJeffrey A. Osterberg0Jillian Milanes1James Morris2Pingshan Wang3Department of Electrical and Computer Engineering, Clemson University, Clemson, SC 29634, USADepartment of Biological Sciences, Clemson University, Clemson, SC 29634, USADepartment of Biological Sciences, Clemson University, Clemson, SC 29634, USADepartment of Electrical and Computer Engineering, Clemson University, Clemson, SC 29634, USA; Corresponding author.Researchers studying cellular life cycles need to be able to monitor the phases of a cell cycle rapidly and accurately. Many of the techniques currently used to monitor the cell cycle require the use of labels and would be difficult to automate. Microwave cytometry is a promising new approach to label free monitoring of cell life cycles. This paper presents results of multiple frequency microwave measurements of two lifecycle stages of Trypanosoma brucei, a unicellular eukaryotic parasite found in sub-Saharan Africa. A microwave flow cytometer was used to show bloodstream form (BSF) and procyclic form (PCF) T. brucei have frequency dependent permittivity and impedance from 800 MHz to 7.65 GHz. The two cell forms had a strong dependence on the imaginary part of permittivity at 2.38 GHz and below and a strong dependence on the real part of permittivity at 5.55 GHz and above. Three PCF cell lines were tested to verify that the differences between the two cell forms were independent of cell strain. Additionally, impedance measurements were used to improve cell classification in cases where the permittivity of a cell cannot be detected. Quadratic discriminate analysis was employed to validate the ability to classify cells forms, with maximum cross-validation errors of 15.4% and 10% when using one and three PCF strains, respectively.http://www.sciencedirect.com/science/article/pii/S2666053922000017Complex permittivityCytometryImpedanceMicrowave sensingTrypanosoma brucei |
| spellingShingle | Jeffrey A. Osterberg Jillian Milanes James Morris Pingshan Wang Detection of Trypanosoma brucei by microwave cytometry Sensors and Actuators Reports Complex permittivity Cytometry Impedance Microwave sensing Trypanosoma brucei |
| title | Detection of Trypanosoma brucei by microwave cytometry |
| title_full | Detection of Trypanosoma brucei by microwave cytometry |
| title_fullStr | Detection of Trypanosoma brucei by microwave cytometry |
| title_full_unstemmed | Detection of Trypanosoma brucei by microwave cytometry |
| title_short | Detection of Trypanosoma brucei by microwave cytometry |
| title_sort | detection of trypanosoma brucei by microwave cytometry |
| topic | Complex permittivity Cytometry Impedance Microwave sensing Trypanosoma brucei |
| url | http://www.sciencedirect.com/science/article/pii/S2666053922000017 |
| work_keys_str_mv | AT jeffreyaosterberg detectionoftrypanosomabruceibymicrowavecytometry AT jillianmilanes detectionoftrypanosomabruceibymicrowavecytometry AT jamesmorris detectionoftrypanosomabruceibymicrowavecytometry AT pingshanwang detectionoftrypanosomabruceibymicrowavecytometry |