Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex
Injury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-08-01
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| Series: | Frontiers in Neural Circuits |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncir.2016.00055/full |
| _version_ | 1811238893005045760 |
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| author | Nissrine Ballout Nissrine Ballout Isabelle Frappe Sophie Peron Mohamed Jaber Kazem Zibara Afsaneh Gaillard Afsaneh Gaillard |
| author_facet | Nissrine Ballout Nissrine Ballout Isabelle Frappe Sophie Peron Mohamed Jaber Kazem Zibara Afsaneh Gaillard Afsaneh Gaillard |
| author_sort | Nissrine Ballout |
| collection | DOAJ |
| description | Injury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent animal models with cortical lesions that grafted fetal cortical neurons could effectively re-establish specific patterns of projections and synapses. The current study was designed to provide a detailed characterization of the spatio-temporal in vivo development of fetal cortical transplanted cells within the lesioned adult motor cortex and their corresponding axonal projections. We show here that as early as two weeks after grafting, cortical neuroblasts transplanted into damaged adult motor cortex developed appropriate projections to cortical and subcortical targets. Grafted cells initially exhibited characteristics of immature neurons, which then differentiated into mature neurons with appropriate cortical phenotypes where most were glutamatergic and few were GABAergic. All cortical subtypes identified with the specific markers CTIP2, Cux1, FOXP2 and Tbr1 were generated after grafting as evidenced with BrdU co-labeling.The set of data provided here is of interest as it sets biological standards for future studies aimed at replacing fetal cells with embryonic stem cells as a source of cortical neurons. |
| first_indexed | 2024-04-12T12:50:15Z |
| format | Article |
| id | doaj.art-1e4b5c3689444f069c3ac3b07878ec42 |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-04-12T12:50:15Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-1e4b5c3689444f069c3ac3b07878ec422022-12-22T03:32:29ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102016-08-011010.3389/fncir.2016.00055190863Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortexNissrine Ballout0Nissrine Ballout1Isabelle Frappe2Sophie Peron3Mohamed Jaber4Kazem Zibara5Afsaneh Gaillard6Afsaneh Gaillard7INSERM U1084ER045, Laboratory of Stem CellsINSERM U1084INSERM U1084INSERM U1084Lebanese universityINSERM U1084University of PoitiersInjury to the human central nervous system can lead to devastating consequences due to its poor ability to self-repair. Neural transplantation aimed at replacing lost neurons and restore functional circuitry has proven to be a promising therapeutical avenue. We previously reported in adult rodent animal models with cortical lesions that grafted fetal cortical neurons could effectively re-establish specific patterns of projections and synapses. The current study was designed to provide a detailed characterization of the spatio-temporal in vivo development of fetal cortical transplanted cells within the lesioned adult motor cortex and their corresponding axonal projections. We show here that as early as two weeks after grafting, cortical neuroblasts transplanted into damaged adult motor cortex developed appropriate projections to cortical and subcortical targets. Grafted cells initially exhibited characteristics of immature neurons, which then differentiated into mature neurons with appropriate cortical phenotypes where most were glutamatergic and few were GABAergic. All cortical subtypes identified with the specific markers CTIP2, Cux1, FOXP2 and Tbr1 were generated after grafting as evidenced with BrdU co-labeling.The set of data provided here is of interest as it sets biological standards for future studies aimed at replacing fetal cells with embryonic stem cells as a source of cortical neurons.http://journal.frontiersin.org/Journal/10.3389/fncir.2016.00055/fullBraincell therapyCortexrepairTrauma |
| spellingShingle | Nissrine Ballout Nissrine Ballout Isabelle Frappe Sophie Peron Mohamed Jaber Kazem Zibara Afsaneh Gaillard Afsaneh Gaillard Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex Frontiers in Neural Circuits Brain cell therapy Cortex repair Trauma |
| title | Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| title_full | Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| title_fullStr | Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| title_full_unstemmed | Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| title_short | Development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| title_sort | development and maturation of embryonic cortical neurons grafted into the damaged adult motor cortex |
| topic | Brain cell therapy Cortex repair Trauma |
| url | http://journal.frontiersin.org/Journal/10.3389/fncir.2016.00055/full |
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