Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach
A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission re...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/6/3/67 |
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| author | Ioannis N. Pasias I. Kiriakou Charalampos Proestos |
| author_facet | Ioannis N. Pasias I. Kiriakou Charalampos Proestos |
| author_sort | Ioannis N. Pasias |
| collection | DOAJ |
| description | A simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission regulations. The accuracy, as provided by recovery experiments, was higher than 93%, and the precision, as provided by the (%) relative standard deviation under reproducibility conditions, was lower than 5%. A vast number of independent parameters that lead in the increase of uncertainty of methods were investigated. The analysis was performed without use of an internal standard, which was proven to be reliable according to several validation methods. The method was applied in real samples, and possible health claims were investigated. |
| first_indexed | 2024-03-12T07:54:35Z |
| format | Article |
| id | doaj.art-1e4b93ac6ce84c43a17acda596b9a63a |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:54:35Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1e4b93ac6ce84c43a17acda596b9a63a2023-09-02T20:19:09ZengMDPI AGAntioxidants2076-39212017-08-01636710.3390/antiox6030067antiox6030067Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated ApproachIoannis N. Pasias0I. Kiriakou1Charalampos Proestos2General Chemical Lab of Research and Analysis, Timfristou 181, 35100 Lamia, GreeceLamia Laboratory, Karaiskaki 85, 35100 Lamia, GreeceLaboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens Panepistimiopolis Zografou, 15771 Athens, GreeceA simple method for the determination of caffeine in coffee grains by GC-FID (Gas Chromatography-Flame Ionisation Detector) is presented in the current work. The method was fully validated according to ISO (International Organization for Standardization) 17025 requirements and European Commission regulations. The accuracy, as provided by recovery experiments, was higher than 93%, and the precision, as provided by the (%) relative standard deviation under reproducibility conditions, was lower than 5%. A vast number of independent parameters that lead in the increase of uncertainty of methods were investigated. The analysis was performed without use of an internal standard, which was proven to be reliable according to several validation methods. The method was applied in real samples, and possible health claims were investigated.https://www.mdpi.com/2076-3921/6/3/67caffeineuncertainty calculationISO 17025Guatemala coffee grainsGC-FID |
| spellingShingle | Ioannis N. Pasias I. Kiriakou Charalampos Proestos Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach Antioxidants caffeine uncertainty calculation ISO 17025 Guatemala coffee grains GC-FID |
| title | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
| title_full | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
| title_fullStr | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
| title_full_unstemmed | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
| title_short | Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID—A Fully Validated Approach |
| title_sort | development of a rapid method for the determination of caffeine in coffee grains by gc fid a fully validated approach |
| topic | caffeine uncertainty calculation ISO 17025 Guatemala coffee grains GC-FID |
| url | https://www.mdpi.com/2076-3921/6/3/67 |
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