Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS)
Introduction: A sensitive and rapid oil-in-water (O/W) microemulsion high performance liquid chromatography (MELC) method has been developed. The water-in-oil (w/o) microemulsion was used as a mobile phase in the determination of salbutamol in aqueous solutions. In addition, the influence of operati...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2013-02-01
|
| Series: | BioImpacts |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.5681/bi.2013.013 |
| _version_ | 1819295201840070656 |
|---|---|
| author | J Hanaee BJ Clark MS Althanyan KH Assi |
| author_facet | J Hanaee BJ Clark MS Althanyan KH Assi |
| author_sort | J Hanaee |
| collection | DOAJ |
| description | Introduction: A sensitive and rapid oil-in-water (O/W) microemulsion high performance liquid chromatography (MELC) method has been developed. The water-in-oil (w/o) microemulsion was used as a mobile phase in the determination of salbutamol in aqueous solutions. In addition, the influence of operating parameters on the separation performance was examined. Methods: The samples were injected into C18, (250mm×4.6mm) analytical columns maintained at 25oC with a flow rate 1 ml/min. The mobile phase was 95.5% v/v aqueous orthophosphate buffer 20 mM (adjusted to pH 3 with orthophosphoric acid), 0.5% ethyl acetate, 1.5% Brij35, and 2.5% 1-butanol, all w/w. The salbutamol and internal standard peaks were detected by fluorescence detection at the excitation and emission wavelengths of 267 and 313 nm respectively. Results: The method had an accuracy of > 97.78% and the calibration curve was linear (r2 = 0.99) over salbutamol concentrations ranging from 25 to 500 ng/mL. The intra-day and inter-day precisions (CV %) were <1.6 and <1.8, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were 9.61ng/ml and 29.13ng/ml, respectively. Conclusion: The method reported is simple, precise and accurate, and has the capacity to be used for determination of salbutamol in the pharmaceutical preparation. |
| first_indexed | 2024-12-24T04:38:27Z |
| format | Article |
| id | doaj.art-1e4e546dbefd4877a8fd7643c9df822a |
| institution | Directory Open Access Journal |
| issn | 2228-5652 2228-5660 |
| language | English |
| last_indexed | 2024-12-24T04:38:27Z |
| publishDate | 2013-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-1e4e546dbefd4877a8fd7643c9df822a2022-12-21T17:14:59ZengTabriz University of Medical SciencesBioImpacts2228-56522228-56602013-02-01313742Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS)J HanaeeBJ ClarkMS AlthanyanKH AssiIntroduction: A sensitive and rapid oil-in-water (O/W) microemulsion high performance liquid chromatography (MELC) method has been developed. The water-in-oil (w/o) microemulsion was used as a mobile phase in the determination of salbutamol in aqueous solutions. In addition, the influence of operating parameters on the separation performance was examined. Methods: The samples were injected into C18, (250mm×4.6mm) analytical columns maintained at 25oC with a flow rate 1 ml/min. The mobile phase was 95.5% v/v aqueous orthophosphate buffer 20 mM (adjusted to pH 3 with orthophosphoric acid), 0.5% ethyl acetate, 1.5% Brij35, and 2.5% 1-butanol, all w/w. The salbutamol and internal standard peaks were detected by fluorescence detection at the excitation and emission wavelengths of 267 and 313 nm respectively. Results: The method had an accuracy of > 97.78% and the calibration curve was linear (r2 = 0.99) over salbutamol concentrations ranging from 25 to 500 ng/mL. The intra-day and inter-day precisions (CV %) were <1.6 and <1.8, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were 9.61ng/ml and 29.13ng/ml, respectively. Conclusion: The method reported is simple, precise and accurate, and has the capacity to be used for determination of salbutamol in the pharmaceutical preparation.http://dx.doi.org/10.5681/bi.2013.013MicroemulsionHPLCMELCDeterminationValidationSalbutamol |
| spellingShingle | J Hanaee BJ Clark MS Althanyan KH Assi Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) BioImpacts Microemulsion HPLC MELC Determination Validation Salbutamol |
| title | Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) |
| title_full | Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) |
| title_fullStr | Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) |
| title_full_unstemmed | Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) |
| title_short | Development of a Microemulsion High Performance Liquid Chromatography (MELC) Method for Determination of Salbutamol in Metered-Dose Inhalers (MDIS) |
| title_sort | development of a microemulsion high performance liquid chromatography melc method for determination of salbutamol in metered dose inhalers mdis |
| topic | Microemulsion HPLC MELC Determination Validation Salbutamol |
| url | http://dx.doi.org/10.5681/bi.2013.013 |
| work_keys_str_mv | AT jhanaee developmentofamicroemulsionhighperformanceliquidchromatographymelcmethodfordeterminationofsalbutamolinmetereddoseinhalersmdis AT bjclark developmentofamicroemulsionhighperformanceliquidchromatographymelcmethodfordeterminationofsalbutamolinmetereddoseinhalersmdis AT msalthanyan developmentofamicroemulsionhighperformanceliquidchromatographymelcmethodfordeterminationofsalbutamolinmetereddoseinhalersmdis AT khassi developmentofamicroemulsionhighperformanceliquidchromatographymelcmethodfordeterminationofsalbutamolinmetereddoseinhalersmdis |