Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in diff...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Fish and Shellfish Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667011921000311 |
| _version_ | 1819100226714075136 |
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| author | Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim |
| author_facet | Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim |
| author_sort | Yi-Teng Xia |
| collection | DOAJ |
| description | A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in different temperatures and serum concentrations: the best growing condition was optimized at 20% serum under 28 °C. In cultured RFM cells, sequencing of 18S rRNA, as well as immunostaining of cytokeratin and CD 68, confirmed the identity as macrophagic cell of S. fuscescens. Cultured RFM cells were exposed to challenge of inflammation, as triggered by LPS, showing highly sensitive responses to inflammation, including release of nitric oxide, expression of cytokine, and activation of phagocytosis. The water extract of aerial part of Scutellaria baicalensis, named as SBA, has been shown anti-inflammatory property in S. fuscescens fish. In order to extend the application of SBA in aquaculture, the extract and its effective flavonoids, i.e. baicalin and scutellarin, were applied in LPS-treated RFM cells. Application of SBA extract, baicalin or scutellarin, inhibited the expressions of LPS-induced inflammatory cytokines, i.e. IL-1β, TNF-α, as well as the signaling of transcription factor NF-κB. The results support the established RFM cell line could be an ideal in vitro model in drug screening relating to inflammation. Additionally, the notion of SBA herbal extract in fish aquaculture is supported by its efficacy against inflammation. |
| first_indexed | 2024-12-22T00:59:25Z |
| format | Article |
| id | doaj.art-1e50b874fce2422699acccda64093cd9 |
| institution | Directory Open Access Journal |
| issn | 2667-0119 |
| language | English |
| last_indexed | 2024-12-22T00:59:25Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fish and Shellfish Immunology Reports |
| spelling | doaj.art-1e50b874fce2422699acccda64093cd92022-12-21T18:44:13ZengElsevierFish and Shellfish Immunology Reports2667-01192021-12-012100036Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensisYi-Teng Xia0Edwin Hok-Chi Cheng1Ying-Jie Xia2Qi-Yun Wu3Lynn Hoi-Lam Zhang4Sheng-Ying Lin5Tina Ting-Xia Dong6Qi-Wei Qin7Wen-Xiong Wang8Karl Wah-Keung Tsim9Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, ChinaJoint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China; School of Energy and Environment, City University of Hong Kong, Kowloon, Hong Kong, ChinaShenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Nanshan, Shenzhen, China; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China; Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China; Corresponding author.A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in different temperatures and serum concentrations: the best growing condition was optimized at 20% serum under 28 °C. In cultured RFM cells, sequencing of 18S rRNA, as well as immunostaining of cytokeratin and CD 68, confirmed the identity as macrophagic cell of S. fuscescens. Cultured RFM cells were exposed to challenge of inflammation, as triggered by LPS, showing highly sensitive responses to inflammation, including release of nitric oxide, expression of cytokine, and activation of phagocytosis. The water extract of aerial part of Scutellaria baicalensis, named as SBA, has been shown anti-inflammatory property in S. fuscescens fish. In order to extend the application of SBA in aquaculture, the extract and its effective flavonoids, i.e. baicalin and scutellarin, were applied in LPS-treated RFM cells. Application of SBA extract, baicalin or scutellarin, inhibited the expressions of LPS-induced inflammatory cytokines, i.e. IL-1β, TNF-α, as well as the signaling of transcription factor NF-κB. The results support the established RFM cell line could be an ideal in vitro model in drug screening relating to inflammation. Additionally, the notion of SBA herbal extract in fish aquaculture is supported by its efficacy against inflammation.http://www.sciencedirect.com/science/article/pii/S2667011921000311Cell line establishmentSiganus fuscescensAnti-inflammationTraditional Chinese medicineScutellaria baicalensis |
| spellingShingle | Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis Fish and Shellfish Immunology Reports Cell line establishment Siganus fuscescens Anti-inflammation Traditional Chinese medicine Scutellaria baicalensis |
| title | Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis |
| title_full | Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis |
| title_fullStr | Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis |
| title_full_unstemmed | Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis |
| title_short | Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis |
| title_sort | characterization of a macrophagic like cell line derived from rabbit fish siganus fuscescens an illustration of anti inflammatory responses of the herbal extract of scutellaria baicalensis |
| topic | Cell line establishment Siganus fuscescens Anti-inflammation Traditional Chinese medicine Scutellaria baicalensis |
| url | http://www.sciencedirect.com/science/article/pii/S2667011921000311 |
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