Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil
Fusarium is widely distributed in the environment and is involved with plant and animal diseases. In humans, several species and species complexes (SC) are related to fusariosis, i.e., F. solani SC, F. oxysporum SC, F. fujikuroi SC, F. dimerum, F. chlamydosporum, F. incarnatum-equiseti, and F. sporo...
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00737/full |
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| author | Patricia F. Herkert Patricia F. Herkert Patricia F. Herkert Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Gabriel L. de Oliveira Salvador Marisol D. Muro Rosângela L. Pinheiro Márcio Nucci Flávio Queiroz-Telles G. Sybren de Hoog G. Sybren de Hoog G. Sybren de Hoog Jacques F. Meis Jacques F. Meis |
| author_facet | Patricia F. Herkert Patricia F. Herkert Patricia F. Herkert Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Gabriel L. de Oliveira Salvador Marisol D. Muro Rosângela L. Pinheiro Márcio Nucci Flávio Queiroz-Telles G. Sybren de Hoog G. Sybren de Hoog G. Sybren de Hoog Jacques F. Meis Jacques F. Meis |
| author_sort | Patricia F. Herkert |
| collection | DOAJ |
| description | Fusarium is widely distributed in the environment and is involved with plant and animal diseases. In humans, several species and species complexes (SC) are related to fusariosis, i.e., F. solani SC, F. oxysporum SC, F. fujikuroi SC, F. dimerum, F. chlamydosporum, F. incarnatum-equiseti, and F. sporotrichoides. We aimed to investigate the susceptibility of Fusarium clinical isolates to antifungals and azole fungicides and identify the species. Forty-three clinical Fusarium isolates were identified by sequencing translation elongation factor 1-alpha (TEF1α) gene. Antifungal susceptibility testing was performed to the antifungals amphotericin B, itraconazole, voriconazole, posaconazole, and isavuconazole, and the azole fungicides difenoconazole, tebuconazole, and propiconazole. The isolates were recovered from patients with median age of 36 years (range 2–78 years) of which 21 were female. Disseminated fusariosis was the most frequent clinical form (n = 16, 37.2%) and acute lymphoblastic leukemia (n = 7; 16.3%) was the most commonly underlying condition. A few species described in Fusarium solani SC have recently been renamed in the genus Neocosmospora, but consistent naming is yet not possible. Fusarium keratoplasticum FSSC 2 (n = 12) was the prevalent species, followed by F. petroliphilum FSSC 1 (n = 10), N. gamsii FSSC 7 (n = 5), N. suttoniana FSSC 20 (n = 3), F. solani sensu stricto FSSC 5 (n = 2), Fusarium sp. FSSC 25 (n = 2), Fusarium sp. FSSC 35 (n = 1), Fusarium sp. FSSC18 (n = 1), F. falciforme FSSC 3+4 (n = 1), F. pseudensiforme (n = 1), and F. solani f. xanthoxyli (n = 1). Amphotericin B had activity against most isolates although MICs ranged from 0.5 to 32 μg mL-1. Fusarium keratoplasticum showed high MIC values (8–>32 μg mL-1) for itraconazole, voriconazole, posaconazole, and isavuconazole. Among agricultural fungicides, difenoconazole had the lowest activity against FSSC with MICs of >32 μg mL-1 for all isolates. |
| first_indexed | 2024-12-11T01:23:34Z |
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| last_indexed | 2024-12-11T01:23:34Z |
| publishDate | 2019-04-01 |
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| spelling | doaj.art-1e51c7b0d7234e038566d8ec88325f512022-12-22T01:25:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-04-011010.3389/fmicb.2019.00737451481Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From BrazilPatricia F. Herkert0Patricia F. Herkert1Patricia F. Herkert2Abdullah M. S. Al-Hatmi3Abdullah M. S. Al-Hatmi4Abdullah M. S. Al-Hatmi5Gabriel L. de Oliveira Salvador6Marisol D. Muro7Rosângela L. Pinheiro8Márcio Nucci9Flávio Queiroz-Telles10G. Sybren de Hoog11G. Sybren de Hoog12G. Sybren de Hoog13Jacques F. Meis14Jacques F. Meis15Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, BrazilInstituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas, Brasília, BrazilCentre of Expertise in Mycology Radboudumc/CWZ, Nijmegen, NetherlandsCentre of Expertise in Mycology Radboudumc/CWZ, Nijmegen, NetherlandsDepartment of Medical Mycology, Westerdijk Fungal Biodiversity Institute, Utrecht, NetherlandsDirectorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, OmanDepartment of Internal Medicine, Federal University of Paraná, Curitiba, BrazilLaboratory of Mycology, Hospital de Clínicas, Federal University of Paraná, Curitiba, BrazilLaboratory of Mycology, Hospital de Clínicas, Federal University of Paraná, Curitiba, BrazilDepartment of Internal Medicine, Hematology Service, University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInfectious Diseases Unit, Department of Public Health, Hospital de Clínicas, Federal University of Paraná, Curitiba, BrazilCentre of Expertise in Mycology Radboudumc/CWZ, Nijmegen, NetherlandsDepartment of Medical Mycology, Westerdijk Fungal Biodiversity Institute, Utrecht, Netherlands0Postgraduate Program in Microbiology, Parasitology and Pathology, Biological Sciences, Department of Basic Pathology, Federal University of Paraná, Curitiba, BrazilCentre of Expertise in Mycology Radboudumc/CWZ, Nijmegen, Netherlands1Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, NetherlandsFusarium is widely distributed in the environment and is involved with plant and animal diseases. In humans, several species and species complexes (SC) are related to fusariosis, i.e., F. solani SC, F. oxysporum SC, F. fujikuroi SC, F. dimerum, F. chlamydosporum, F. incarnatum-equiseti, and F. sporotrichoides. We aimed to investigate the susceptibility of Fusarium clinical isolates to antifungals and azole fungicides and identify the species. Forty-three clinical Fusarium isolates were identified by sequencing translation elongation factor 1-alpha (TEF1α) gene. Antifungal susceptibility testing was performed to the antifungals amphotericin B, itraconazole, voriconazole, posaconazole, and isavuconazole, and the azole fungicides difenoconazole, tebuconazole, and propiconazole. The isolates were recovered from patients with median age of 36 years (range 2–78 years) of which 21 were female. Disseminated fusariosis was the most frequent clinical form (n = 16, 37.2%) and acute lymphoblastic leukemia (n = 7; 16.3%) was the most commonly underlying condition. A few species described in Fusarium solani SC have recently been renamed in the genus Neocosmospora, but consistent naming is yet not possible. Fusarium keratoplasticum FSSC 2 (n = 12) was the prevalent species, followed by F. petroliphilum FSSC 1 (n = 10), N. gamsii FSSC 7 (n = 5), N. suttoniana FSSC 20 (n = 3), F. solani sensu stricto FSSC 5 (n = 2), Fusarium sp. FSSC 25 (n = 2), Fusarium sp. FSSC 35 (n = 1), Fusarium sp. FSSC18 (n = 1), F. falciforme FSSC 3+4 (n = 1), F. pseudensiforme (n = 1), and F. solani f. xanthoxyli (n = 1). Amphotericin B had activity against most isolates although MICs ranged from 0.5 to 32 μg mL-1. Fusarium keratoplasticum showed high MIC values (8–>32 μg mL-1) for itraconazole, voriconazole, posaconazole, and isavuconazole. Among agricultural fungicides, difenoconazole had the lowest activity against FSSC with MICs of >32 μg mL-1 for all isolates.https://www.frontiersin.org/article/10.3389/fmicb.2019.00737/fullfusariosisantifungalfungicidesusceptibilityFusariummolecular identification |
| spellingShingle | Patricia F. Herkert Patricia F. Herkert Patricia F. Herkert Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Abdullah M. S. Al-Hatmi Gabriel L. de Oliveira Salvador Marisol D. Muro Rosângela L. Pinheiro Márcio Nucci Flávio Queiroz-Telles G. Sybren de Hoog G. Sybren de Hoog G. Sybren de Hoog Jacques F. Meis Jacques F. Meis Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil Frontiers in Microbiology fusariosis antifungal fungicide susceptibility Fusarium molecular identification |
| title | Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil |
| title_full | Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil |
| title_fullStr | Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil |
| title_full_unstemmed | Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil |
| title_short | Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil |
| title_sort | molecular characterization and antifungal susceptibility of clinical fusarium species from brazil |
| topic | fusariosis antifungal fungicide susceptibility Fusarium molecular identification |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00737/full |
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