Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021
Ailong Huang,* Yuchao Wang,* Haiyan Xu, Xiuxiu Jin, Bingqing Yan, Wei Zhang Department of Microbiology, Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei Zhang, Dep...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-12-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/antibiotic-resistance-and-epidemiology-of-vibrio-parahaemolyticus-from-peer-reviewed-fulltext-article-IDR |
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| author | Huang A Wang Y Xu H Jin X Yan B Zhang W |
| author_facet | Huang A Wang Y Xu H Jin X Yan B Zhang W |
| author_sort | Huang A |
| collection | DOAJ |
| description | Ailong Huang,* Yuchao Wang,* Haiyan Xu, Xiuxiu Jin, Bingqing Yan, Wei Zhang Department of Microbiology, Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei Zhang, Department of Microbiology, Nantong Center for Disease Control and Prevention, No. 189, Gongnong South Road, Chongchuan District, Nantong City, Jiangsu Province, People’s Republic of China, Email zhangwei_1886@sina.comPurpose: The objective of this study was to investigate the prevalence and molecular characteristics of Vibrio parahaemolyticus isolates from fecal samples of patients in Nantong, China.Methods: From 2018 to 2021, a total of 106 clinical cases and samples of V. parahaemolyticus infection were collected. The virulence genes, serotypes and antibiotic resistance of these isolates were analyzed. Additionally, pulsed-field gel electrophoresis (PFGE) was used to analyze the homogeneity of the isolates.Results: Outbreaks of V. parahaemolyticus infection were concentrated in the summer, with seafood consumption being the primary contributing factor, followed by meat and meat products. tlh+tdh+trh- was confirmed as the most frequently detected virulence genotype among the clinical isolates. 16 serotypes were identified, and O3:K6 was the dominant serotype in Nantong. The antimicrobial susceptibility testing revealed the highest resistance rate to cefazolin (99.1%, 104/106), followed by ampicillin (64.2%, 68/106) and tetracycline (29.2%, 31/106). Fourteen resistant phenotypes were identified, with ampicillin-cefazolin being the most prevalent. The multiple antibiotic resistance (MAR) index ranged from 0.07 to 0.36. PFGE typing clustered isolates with similarity greater than 85% into ten genetic clusters (A-J).Conclusion: Clinical isolates generally exhibited pathogenicity and drug resistance, with some isolates displaying high homology. Clusters C, E, and G were the predominant circulating clusters in this area, posing a potential risk of recurrent outbreaks, which demanded our vigilance.Keywords: Vibrio parahaemolyticus, clinical isolates, antibiotic resistance, PFGE |
| first_indexed | 2024-03-09T02:44:56Z |
| format | Article |
| id | doaj.art-1e51c8ef031f48009e048305f2fcb683 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-03-09T02:44:56Z |
| publishDate | 2023-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-1e51c8ef031f48009e048305f2fcb6832023-12-05T17:06:55ZengDove Medical PressInfection and Drug Resistance1178-69732023-12-01Volume 167413742588695Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021Huang AWang YXu HJin XYan BZhang WAilong Huang,* Yuchao Wang,* Haiyan Xu, Xiuxiu Jin, Bingqing Yan, Wei Zhang Department of Microbiology, Nantong Center for Disease Control and Prevention, Nantong, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei Zhang, Department of Microbiology, Nantong Center for Disease Control and Prevention, No. 189, Gongnong South Road, Chongchuan District, Nantong City, Jiangsu Province, People’s Republic of China, Email zhangwei_1886@sina.comPurpose: The objective of this study was to investigate the prevalence and molecular characteristics of Vibrio parahaemolyticus isolates from fecal samples of patients in Nantong, China.Methods: From 2018 to 2021, a total of 106 clinical cases and samples of V. parahaemolyticus infection were collected. The virulence genes, serotypes and antibiotic resistance of these isolates were analyzed. Additionally, pulsed-field gel electrophoresis (PFGE) was used to analyze the homogeneity of the isolates.Results: Outbreaks of V. parahaemolyticus infection were concentrated in the summer, with seafood consumption being the primary contributing factor, followed by meat and meat products. tlh+tdh+trh- was confirmed as the most frequently detected virulence genotype among the clinical isolates. 16 serotypes were identified, and O3:K6 was the dominant serotype in Nantong. The antimicrobial susceptibility testing revealed the highest resistance rate to cefazolin (99.1%, 104/106), followed by ampicillin (64.2%, 68/106) and tetracycline (29.2%, 31/106). Fourteen resistant phenotypes were identified, with ampicillin-cefazolin being the most prevalent. The multiple antibiotic resistance (MAR) index ranged from 0.07 to 0.36. PFGE typing clustered isolates with similarity greater than 85% into ten genetic clusters (A-J).Conclusion: Clinical isolates generally exhibited pathogenicity and drug resistance, with some isolates displaying high homology. Clusters C, E, and G were the predominant circulating clusters in this area, posing a potential risk of recurrent outbreaks, which demanded our vigilance.Keywords: Vibrio parahaemolyticus, clinical isolates, antibiotic resistance, PFGEhttps://www.dovepress.com/antibiotic-resistance-and-epidemiology-of-vibrio-parahaemolyticus-from-peer-reviewed-fulltext-article-IDRvibrio parahaemolyticusclinical isolatesantibiotic resistancepfge |
| spellingShingle | Huang A Wang Y Xu H Jin X Yan B Zhang W Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 Infection and Drug Resistance vibrio parahaemolyticus clinical isolates antibiotic resistance pfge |
| title | Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 |
| title_full | Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 |
| title_fullStr | Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 |
| title_full_unstemmed | Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 |
| title_short | Antibiotic Resistance and Epidemiology of Vibrio parahaemolyticus from Clinical Samples in Nantong, China, 2018–2021 |
| title_sort | antibiotic resistance and epidemiology of vibrio parahaemolyticus from clinical samples in nantong china 2018 ndash 2021 |
| topic | vibrio parahaemolyticus clinical isolates antibiotic resistance pfge |
| url | https://www.dovepress.com/antibiotic-resistance-and-epidemiology-of-vibrio-parahaemolyticus-from-peer-reviewed-fulltext-article-IDR |
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