The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver
A procedure for the hydrolysis of phospholipids is described. The effect of alkali concentration, organic solvents, and length of hydrolysis have been studied. The initial reaction in the preferred solvent system appears to be a methanolysis. The phospholipids are converted quantitatively into water...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1960-10-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520412295 |
| _version_ | 1818931479358472192 |
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| author | G. Hübscher J.N. Hawthorne P. Kemp |
| author_facet | G. Hübscher J.N. Hawthorne P. Kemp |
| author_sort | G. Hübscher |
| collection | DOAJ |
| description | A procedure for the hydrolysis of phospholipids is described. The effect of alkali concentration, organic solvents, and length of hydrolysis have been studied. The initial reaction in the preferred solvent system appears to be a methanolysis. The phospholipids are converted quantitatively into water-soluble phosphates, which are separated and estimated by ion-exchange chromatography. In this way phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol may be determined in liver tissue. |
| first_indexed | 2024-12-20T04:17:15Z |
| format | Article |
| id | doaj.art-1e55d5238aae41969711285b0c5e34be |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T04:17:15Z |
| publishDate | 1960-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-1e55d5238aae41969711285b0c5e34be2022-12-21T19:53:44ZengElsevierJournal of Lipid Research0022-22751960-10-0115433438The analysis of tissue phospholipids: hydrolysis procedure and results with pig liverG. Hübscher0J.N. Hawthorne1P. Kemp2Department of Medical Biochemistry and Pharmacology The Medical School, Birmingham 15, EnglandDepartment of Medical Biochemistry and Pharmacology The Medical School, Birmingham 15, EnglandDepartment of Medical Biochemistry and Pharmacology The Medical School, Birmingham 15, EnglandA procedure for the hydrolysis of phospholipids is described. The effect of alkali concentration, organic solvents, and length of hydrolysis have been studied. The initial reaction in the preferred solvent system appears to be a methanolysis. The phospholipids are converted quantitatively into water-soluble phosphates, which are separated and estimated by ion-exchange chromatography. In this way phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol may be determined in liver tissue.http://www.sciencedirect.com/science/article/pii/S0022227520412295 |
| spellingShingle | G. Hübscher J.N. Hawthorne P. Kemp The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver Journal of Lipid Research |
| title | The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver |
| title_full | The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver |
| title_fullStr | The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver |
| title_full_unstemmed | The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver |
| title_short | The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver |
| title_sort | analysis of tissue phospholipids hydrolysis procedure and results with pig liver |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520412295 |
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