Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples

Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples

Abstract Background Whole-genome sequencing (WGS) is becoming increasingly useful to study the biology, epidemiology, and ecology of malaria parasites. Despite ease of sampling, DNA extracted from dried blood spots (DBS) has a high ratio of human DNA compared to parasite DNA, which poses a challenge...

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Bibliographic Details
Main Authors: Noam B. Teyssier, Anna Chen, Elias M. Duarte, Rene Sit, Bryan Greenhouse, Sofonias K. Tessema
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Malaria Journal
Subjects:
Malaria
P. falciparum
Dried blood spots
Tween-Chelex
McrBC
Selective whole genome amplification
Online Access:https://doi.org/10.1186/s12936-021-03630-4

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