The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
Vimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.970034/full |
| _version_ | 1798004551072088064 |
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| author | Francesca Soglia Martina Bordini Maurizio Mazzoni Martina Zappaterra Mattia Di Nunzio Paolo Clavenzani Roberta Davoli Adele Meluzzi Federico Sirri Massimiliano Petracci |
| author_facet | Francesca Soglia Martina Bordini Maurizio Mazzoni Martina Zappaterra Mattia Di Nunzio Paolo Clavenzani Roberta Davoli Adele Meluzzi Federico Sirri Massimiliano Petracci |
| author_sort | Francesca Soglia |
| collection | DOAJ |
| description | Vimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscles (PM) of fast-growing (FG) and medium-growing (MG) meat-type chickens both at the protein and gene levels. MG broilers were considered as a control group whereas the evolution of VIM and DES over the growth period was evaluated in FG by collecting samples at different developmental stages (7, 14, 21, 28, 35, and 42 days). After performing a preliminary classification of the samples based on their histological features, 5 PM/sampling time/genotype were selected for western blot, immunohistochemistry (IHC), and gene expression analyses. Overall, the findings obtained at the protein level mirrored those related to their encoding genes, although a potential time lag required to observe the consequences of gene expression was evident. The two- and 3-fold higher level of the VIM-based heterodimer observed in FG at d 21 and d 28 in comparison with MG of the same age might be ascribed to the beginning and progressive development of the regenerative processes. This hypothesis is supported by IHC highlighting the presence of fibers to co-expressing VIM and DES. In addition, gene expression analyses suggested that, unlike VIM common sequence, VIM long isoform may not be directly implicated in muscle regeneration. As for DES content, the fluctuating trends observed for both the native protein and its heterodimer in FG might be ascribed to its importance for maintaining the structural organization of the regenerating fibers. Furthermore, the higher expression level of the DES gene in FG in comparison with MG further supported its potential application as a marker of muscle fibers’ regeneration. In conclusion, the findings of the present research seem to support the existence of a relationship between the occurrence of muscle regeneration and the growth rate of meat-type chickens and corroborate the potential use of VIM and DES as molecular markers of these cellular processes. |
| first_indexed | 2024-04-11T12:25:30Z |
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| id | doaj.art-1e5c32ca1246432daae68236cfc565b6 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T12:25:30Z |
| publishDate | 2022-09-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-1e5c32ca1246432daae68236cfc565b62022-12-22T04:23:56ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-09-011310.3389/fphys.2022.970034970034The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regenerationFrancesca Soglia0Martina Bordini1Maurizio Mazzoni2Martina Zappaterra3Mattia Di Nunzio4Paolo Clavenzani5Roberta Davoli6Adele Meluzzi7Federico Sirri8Massimiliano Petracci9Department of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyDepartment of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum–University of Bologna, Bologna, ItalyVimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscles (PM) of fast-growing (FG) and medium-growing (MG) meat-type chickens both at the protein and gene levels. MG broilers were considered as a control group whereas the evolution of VIM and DES over the growth period was evaluated in FG by collecting samples at different developmental stages (7, 14, 21, 28, 35, and 42 days). After performing a preliminary classification of the samples based on their histological features, 5 PM/sampling time/genotype were selected for western blot, immunohistochemistry (IHC), and gene expression analyses. Overall, the findings obtained at the protein level mirrored those related to their encoding genes, although a potential time lag required to observe the consequences of gene expression was evident. The two- and 3-fold higher level of the VIM-based heterodimer observed in FG at d 21 and d 28 in comparison with MG of the same age might be ascribed to the beginning and progressive development of the regenerative processes. This hypothesis is supported by IHC highlighting the presence of fibers to co-expressing VIM and DES. In addition, gene expression analyses suggested that, unlike VIM common sequence, VIM long isoform may not be directly implicated in muscle regeneration. As for DES content, the fluctuating trends observed for both the native protein and its heterodimer in FG might be ascribed to its importance for maintaining the structural organization of the regenerating fibers. Furthermore, the higher expression level of the DES gene in FG in comparison with MG further supported its potential application as a marker of muscle fibers’ regeneration. In conclusion, the findings of the present research seem to support the existence of a relationship between the occurrence of muscle regeneration and the growth rate of meat-type chickens and corroborate the potential use of VIM and DES as molecular markers of these cellular processes.https://www.frontiersin.org/articles/10.3389/fphys.2022.970034/fullbroiler chickengrowth-rateimmunohistochemistrywestern blotgene expression |
| spellingShingle | Francesca Soglia Martina Bordini Maurizio Mazzoni Martina Zappaterra Mattia Di Nunzio Paolo Clavenzani Roberta Davoli Adele Meluzzi Federico Sirri Massimiliano Petracci The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration Frontiers in Physiology broiler chicken growth-rate immunohistochemistry western blot gene expression |
| title | The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| title_full | The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| title_fullStr | The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| title_full_unstemmed | The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| title_short | The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| title_sort | evolution of vimentin and desmin in pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration |
| topic | broiler chicken growth-rate immunohistochemistry western blot gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.970034/full |
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